Renal Transplantation in the Rat: III. The Effect of Colloidal and Non-colloidal Perforates on the Clearing of Blood from the in vitro Perfuse Rat Kidney

Frødin, L

doi:10.3109/00365597509139919

Cited by 8 publications

(2 citation statements)

References 8 publications

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“…Notably, this solution can be supplemented with RBCs or remain acellular ( 41 ). The armamentarium of perfusates furthermore involves a wide array of modifications, such as the Custodiol-MP histidine–tryptophan–ketoglutarate (HTK) solution with high-flow, low-potassium, and anti-nitrosative/oxidant properties designed for oxygenated EVMP at 4°C ( 47 ), the cellular Organ Care System solution with a low-potassium dextran formula (at 37°C) ( 48 ), Perfadex as an extracellular and dextran-based electrolyte preservation solution (at 37°C–38°C) ( 49 ), or the Celsior solution as a colloid-free extracellular-type solution (at 2°C–8°C) ( 50 ). Next to the abovementioned preservation solutions, various others have been described ( 8 , 51 , 52 ).…”

Section: Ex Situ Machine Perfusionmentioning

confidence: 99%

Pushing the boundaries of innovation: the potential of ex vivo organ perfusion from an interdisciplinary point of view

Iske,

Schroeter,

Knoedler

et al. 2023

Front. Cardiovasc. Med.

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Ex vivo machine perfusion (EVMP) is an emerging technique for preserving explanted solid organs with primary application in allogeneic organ transplantation. EVMP has been established as an alternative to the standard of care static-cold preservation, allowing for prolonged preservation and real-time monitoring of organ quality while reducing/preventing ischemia–reperfusion injury. Moreover, it has paved the way to involve expanded criteria donors, e.g., after circulatory death, thus expanding the donor organ pool. Ongoing improvements in EVMP protocols, especially expanding the duration of preservation, paved the way for its broader application, in particular for reconditioning and modification of diseased organs and tumor and infection therapies and regenerative approaches. Moreover, implementing EVMP for in vivo-like preclinical studies improving disease modeling raises significant interest, while providing an ideal interface for bioengineering and genetic manipulation. These approaches can be applied not only in an allogeneic and xenogeneic transplant setting but also in an autologous setting, where patients can be on temporary organ support while the diseased organs are treated ex vivo, followed by reimplantation of the cured organ. This review provides a comprehensive overview of the differences and similarities in abdominal (kidney and liver) and thoracic (lung and heart) EVMP, focusing on the organ-specific components and preservation techniques, specifically on the composition of perfusion solutions and their supplements and perfusion temperatures and flow conditions. Novel treatment opportunities beyond organ transplantation and limitations of abdominal and thoracic EVMP are delineated to identify complementary interdisciplinary approaches for the application and development of this technique.

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Section: Ex Situ Machine Perfusionmentioning

confidence: 99%

Pushing the boundaries of innovation: the potential of ex vivo organ perfusion from an interdisciplinary point of view

Iske,

Schroeter,

Knoedler

et al. 2023

Front. Cardiovasc. Med.

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“…Quite a few investigators [6,12] performed kidney preservation experiments in the rat with the homologous transplantation technique. Excellent functional results of the transplant can only be achieved with extensive microsurgical training and skill.…”

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confidence: 99%

Orthotopic and heterotopic autotransplantation of the rat kidney

1978

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End-to-end anastomosis of the kidney vessels and ureter can easily be done without suture. Artery and ureter are connected via an intraluminal silicone tube, anastomosis of the vein is performed with a cuff-technique. In case of orthotopic transplantation the left kidney is transplanted to the right side after right nephrectomy. Furthermore heterotopic transplantation was tried: kidney vessels were either connected with jugular or femoral vessels. In contrast to orthotopic transplantation higher thrombosis rate was observed, probably due to varying sizes of donor and recipient vessels. Our method of orthotopic autotransplantation is easier, less time consuming and has a lower complication rate than homologous kidney transplantation with suture techniques.

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