Renal Vasoconstriction in Glycerol-Induced Acute Renal Failure. Studies in the Isolated Perfused Rat Kidney

Hofbauer, K. G.; Bauereiss, K.; Konrads, A.; Gross, F.

doi:10.1042/cs0550249

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…This was associated with liver dysfunction as indicated by PDR of DY-780 to a similar degree as in liver IR injury demonstrating remote organ dysfunction of the liver concomitant with renal dysfunction, rendering organ-organ interactions in multi-organ dysfunction syndroms accessible to real-time monitoring. Finally, (failing) conjugation of bile acids also indicated liver dysfunction as previously described 45 and correlated with biophotonic assessment of excretory failure in models of mild sepsis and liver IR. Similarly, elevation of unconjugated bile acids in plasma indicates an intrahepatic failure of phase I and II biotransformation in severe sepsis and kidney IR.…”

Section: Discussionsupporting

confidence: 67%

“…Concomitantly, this severe glomerular damage models also resulted in an elevated DY-654 plasma-peak concentration. Alterations in renal vascular resistance in the model of glycerol-induced acute kidney injury were described previously 45 . For this model, confocal intravital microscopy further revealed occurrence of cellular debris in tubular lumen and necrotic tubular cells, identified as DY-654 and PI positive, as well as necrotic tubular endothelial cells (DY-654 negative and PI positive), thus providing additional spatial and cell-type specific information regarding occurrence of injury and dysfunction.…”

Section: Discussionmentioning

confidence: 99%

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Fast simultaneous assessment of renal and liver function using polymethine dyes in animal models of chronic and acute organ injury

Press

Butans

Haider

et al. 2017

Sci Rep

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Simultaneous assessment of excretory liver and kidney function is still an unmet need in experimental stress models as well as in critical care. The aim of the study was to characterize two polymethine-dyes potentially suitable for this purpose in vivo. Plasma disappearance rate and elimination measurements of simultaneously injected fluorescent dyes DY-780 (hepato-biliary elimination) and DY-654(renal elimination) were conducted using catheter techniques and intravital microscopy in animals subjected to different organ injuries, i.e. polymicrobial sepsis by peritoneal contamination and infection, ischemia-reperfusion-injury and glycerol-induced acute kidney-injury. DY-780 and DY-654 showed organ specific and determined elimination routes in both healthy and diseased animals. They can be measured simultaneously using near-infrared imaging and spectrophotometry. Plasma-disappearance rates of DY-780 and DY-654 are superior to conventional biomarkers in indicating hepatic or kidney dysfunction in different animal models. Greatest impact on liver function was found in animals with polymicrobial sepsis whereas glomerular damage due to glycerol-induced kidney-injury had strongest impact on DY-654 elimination. We therefore conclude that hepatic elimination and renal filtration can be assessed in rodents measuring plasma-disappearance rates of both dyes. Further, assessment of organ dysfunction by polymethine dyes correlates with, but outperforms conventional biomarkers regarding sensitivity and the option of spatial resolution if biophotonic strategies are applied. Polymethine-dye clearance thereby allows sensitive point-of-care assessment of both organ functions simultaneously.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Fast simultaneous assessment of renal and liver function using polymethine dyes in animal models of chronic and acute organ injury

Press

Butans

Haider

et al. 2017

Sci Rep

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show abstract

“…During the first 2-3 h after ARF induction, systemic and renal hemodynamic changes have been found: plasma volume reduction [26], decrease of CO and increase of TPR [9,11], increase of MAP [2,9,24], reduction of RBF [1,2,15] with intrarenal blood flow redistribution and cortical ischemia [I] and increase in RVR [10,12].…”

Section: Discussionmentioning

confidence: 99%

Role of Hemodynamic Alterations in the Partial Protection Afforded by Uninephrectomy against Glycerol-Induced Acute Renal Failure in Rats

Ramos¹,

López‐Novoa²,

Hernando³

1982

Nephron

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Systemic and renal hemodynamics and body fluid volumes were evaluated in 48-hour uninephrectomized (UNX) and sham-operated (SO) rats, 3 h after glycerol-induced acute renal failure (ARF). UNX rats showed a 20% increase in cardiac output (CO) and renal blood flow (RBF) and a 20% decrease in total peripheric resistance (TPR) and renal vascular resistance (RVR) with respect to the control rats. There was no difference in body fluid volumes. 3 h after ARF induction UNX rats showed a minor decrease in RBF and GFR and a minor increase in RVR when compared with the control rats. CO decreased to similar levels in both UNX and SO rats. Mean arterial pressure and TPR increases were observed in UNX and SO rats but these increases were higher in the UNX than in the SO rats. Plasma and extracellular volumes were reduced in both groups of rats. The partial protection afforded by the renal mass reduction against the glycerol-induced ARF seem to be explained by the minor decrease in RBF and the lesser increase in RVR exhibited by the UNX rats after ARF induction.

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Glycerol-Induced Acute Renal Failure in Brattleboro Rats with Hypothalamic Diabetes Insipidus

et al. 1979

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1. During the development of glycerol-induced acute renal failure in Sprague-Dawley rats, plasma concentrations of vasopressin rise and probably induce an increase in blood pressure. 2.In the present studies the role of vasopressin in acute renal failure was further analysed by experiments in Brattleboro rats homozygous for hereditary hypothalamic diabetes insipidus which were injected intramuscularly with 10 ml of glycerol/kg (6 1 mmoV1).3. After the injection of glycerol plasma osmolality increased transiently and packed cell volume was elevated. The rats became anuric and plasma urea concentrations rose progressively. Plasma renin concentration increased significantly within 2 h. Plasma renin substrate concentration rose progressively and had almost doubled by 8 h.4. In contrast with previous observations in Sprague-Dawley rats, blood pressure did not rise in rats with diabetes insipidus after the injection of glycerol.5. When 2 h after the injection of glycerol kidneys were taken from rats with diabetes insipidus and perfused with an electrolyte solution in a single-pass system for 1 h, renal vascular resistance was 30% higher than in control kidneys 10 min after the start of the perfusion and remained elevated thereafter. In similar experiments with kidneys from Sprague-Dawley rats with acute renal failure, renal vascular resistance was increased fivefold immediately after the start of the perfusion, but decreased subsequently.6. These data support the idea that in glycerolinduced acute renal failure of Sprague-Dawley rats an increased release of vasopressin is responsible for the elevation of blood pressure and suggest that this hormone also participates in renal vasoconstriction. However, a rise of plasma vasopressin concentrations alone cannot fully explain the increase in renal vascular resistance and the development of acute renal failure.

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Renal Vasoconstriction in Glycerol-Induced Acute Renal Failure. Studies in the Isolated Perfused Rat Kidney

Cited by 3 publications

References 13 publications

Fast simultaneous assessment of renal and liver function using polymethine dyes in animal models of chronic and acute organ injury

Fast simultaneous assessment of renal and liver function using polymethine dyes in animal models of chronic and acute organ injury

Role of Hemodynamic Alterations in the Partial Protection Afforded by Uninephrectomy against Glycerol-Induced Acute Renal Failure in Rats

Glycerol-Induced Acute Renal Failure in Brattleboro Rats with Hypothalamic Diabetes Insipidus

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