Renewal of Goblet and Paneth Cells in the Small Intestine

Cairnie, A. B.

doi:10.1111/j.1365-2184.1970.tb00250.x

Cited by 27 publications

(30 citation statements)

References 11 publications

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“…The exact point during cell maturation at which the Paneth cells appear is also unknown, as is whether their progenitors move immediately downwards (Hampton 1968), or initially move upwards with the general cell flow and then move down to the crypt base. However, the fully differentiated Paneth cells appear after 3 days (Cairnie 1970) and, unlike the other upwardly moving cell types, have a much longer lifetime within the animal, lasting for over two weeks (when they appear to be removed by phagocytosis, Troughton & Trier 1969;Cheng & Leblond 1974a). The intercalated cells (non-Paneth cells located near the crypt base) may be early Paneth cells (Hampton 1968), although some have argued these are actually stem cells (Bjerknes & Cheng 1981).…”

Section: Differentiated Phenotypesmentioning

confidence: 99%

“…The usefulness of this type of stem cell functional assay was first described by Till & McCulloch in 1961 as a spleen colony assay for haemopoietic stem cells. The philosophy was then adapted to the gut as a macro (late times) and then micro (early times) colony assay (Withers & Elkind 1969, 1970Potten & Hendry 1985). This either measures the gross morphological regeneration of the denuded gut following high dose irradiation (macro) or the regeneration of individual crypts (micro).…”

Section: Clonogenic Cell Estimates From In Vivo Regeneration Modelsmentioning

confidence: 99%

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The intestinal epithelial stem cell: the mucosal governor

Potten

Booth

Pritchard

1997

Int J Experimental Path

445

326

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Summary. All epithelial cells in the small and large intestine are thought to originate from stem cells located towards the base of the crypts of Lieberkü hn. To-date, there are no specific intestinal stem cell markers, hence stem cell properties can only be inferred. A range of experimental techniques have been employed including cell position mapping, radiation regeneration (clonogenic) assays, chimeric and transgenic mice. This review discusses the implications of experiments performed using these techniques in order to deduce the number, location and functional properties of stem cells. Stem cell homeostasis is maintained by cell proliferation and death 'through apoptosis'. The various growth and matrix factors and genes which may control these processes, and be important for stem cell function, are discussed along with their carcinogenic and clinical implications.Keywords: stem cell, intestine, apoptosis, epithelium Proliferative organization of the intestinal epitheliumThe lumenal surface of the intestine is lined by a simple columnar epithelium. The surface of both the small and large intestine is folded into a number of deep cavities or crypts of Lieberkü hn, which are embedded in connective tissue. In the small intestine the surface area is further increased by finger-like projections, or villi, also covered by epithelium, which protrude into the gut lumen. Although the crypts are approximately 10 × smaller than the villi, they are much more numerous (7 × more in the duodenum, 4 × more in the ileum, Wright & Alison 1984). The effect of these numerous 'pits and projections' is to form a huge surface area. This surface area is further expanded by the presence of a carpet-like covering of microvilli on the apical (lumenal) surface of the columnar epithelial cells.The functional cells of the epithelium are located predominantly towards the lumenal pole of the cryptvillus axis (i.e. on the villi of the small intestine and at the top of the crypts of the large intestine). Once they have fulfilled their differentiated function (brush border enzyme secretion, mucus secretion, nutrient and water absorption) the cells senesce and are sloughed off into the lumen itself, possibly after initiating some aspects of the Int.

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Section: Differentiated Phenotypesmentioning

confidence: 99%

Section: Clonogenic Cell Estimates From In Vivo Regeneration Modelsmentioning

confidence: 99%

The intestinal epithelial stem cell: the mucosal governor

Potten

Booth

Pritchard

1997

Int J Experimental Path

445

326

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“…To date, it has been shown that the Paneth cell has no self-replicating ability (THROUGHTON and TRIER, 1969;CHENG et al, 1969, CAIRNIE, 1970CHENG, 1974b), and seems to be derived from crypt-base columnar cells and degenerate in situ (CHENG at al., 1969;CHENG, 1974b). The turnover time of the population is thought to be much longer than that of goblet cells (CHENG et al, 1969;CAIRNIE, 1970;CHENG, 1974 a, b). …”

Section: The Paneth Cellmentioning

confidence: 99%

“…Although a few goblet cells are capable of mitosis (CAIRNIE, 1970;SHIMAMOTO, 1970), a majority is produced from generative cells in crypts. Labelled goblet cells appear in the villus 20-24 hrs later than labelled columnar cells after 3H-thymidine administration (MERZEL and LEBLOND, 1969;CAIRNIE, 1970). Both cells, however, migrate along the villi at the same speed.…”

mentioning

confidence: 99%

Cellular kinetics of gastrointestinal mucosa, with special reference to gut endocrine cells.

Inokuchi

Fujimoto

Kawai

1983

Arch. Histol. Jap., Arch. Histol. Jpn

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“…However, it cannot be excluded that the mucous cells may also derive from proliferating crypt-base columnar cells (14). The decision to study goblet cell behaviour was also made in order to examine the cellular differentiation process in the small intestine after irradiation.…”

mentioning

confidence: 99%