Renewal rate and nutrient concentration as tools to modify productivity and biochemical composition of cyclostat cultures of the marine microalga Dunaliella tertiolecta

Fábregas, Jaime; Patiño, Manuel R. Bermejo; Arredondo-Vega, Bertha; Tobar, J. L.; Otero, Agustín Godás

doi:10.1007/s002530050556

Cited by 11 publications

(17 citation statements)

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“…Our results agree with studies on the repeated batch cultivation of the microalga Tetraselmis suecica (Fa´bregas et al 1995b) using a daily medium renewal rate of 50%, in this case the biomass productivity (P X ) reached 0.216 g l )1 day )1 . Similar results have also been obtained in studies involving the repeated batch cultivation of Dunaliella tertiolecta (Fa´bregas et al 1995a), Phaeodactylum tricornutum (Fa´bregas et al 1996a) and Haematococcus pluvialis (Fa´bregas et al 2001). In our batch cultivation runs we found that the biomass productivity of strain LEB-52 in Medium B (1:1 MLW:Zarrouk's medium) was significantly better (P < 0.05) than under the other conditions tested.…”

Section: Runsupporting

confidence: 79%

“…According to Otero et al (1998), the use of repeated batch culture provides a simple tool under different light/dark cycles, avoiding possible interference caused by continuous dilution. Repeated batch cultivation also has operational advantages, such as avoiding variation in the inoculum and maintaining the microorganism at high growth rates (Fa´bregas et al 1996a(Fa´bregas et al , 1995a. Although repeated batch cultivation is widely used, the use of this methodology for the cultivation of microalgae and cyanobacteria has been little studied, few papers having been published on the effects of cell concentration or medium renewal-rate.…”

Section: Introductionmentioning

confidence: 98%

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Repeated batch cultivation of the microalga Spirulina platensis

Reinehr

Costa

2006

World J Microbiol Biotechnol

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The cultivation of photosynthetic microorganisms such as the microalga Spirulina platensis can provide an alternative source of food. The water in Mangueira Lagoon (Rio Grande do Sul state, southern Brazil) has several required nutrients for the growth of Spirulina and could be added to culture medium to reduce the cost of producing S. platensis. Although little studied, repeated batch cultivation is a very useful technique because it has a better cost-benefit ratio than other cultivation methods. In a series of runs, we studied the influence of cell concentration, renewal rate and strain on the specific growth rate and biomass productivity of S. platensis during repeated batch cultivation, the runs taking place in 2-l Erlenmeyer flasks for 2160 h at 30°C and a light intensity of 2500 lux under a 12 h photoperiod. The three factors studied had a significant (P < 0.05) effect on the results (specific growth rate and productivity). Using Zarrouk's medium, the highest specific growth rate (l X ) was 0.111 day )1 while the biomass productivity (P X ) was 0.0423 g l )1 day )1 , while Mangueira Lagoon water supplemented with 10% Zarrouk's medium gave l X = 0.113 day )1 and a productivity P X = 0.0467 g l )1 day )1 . These values were two to three times higher than the results obtained in batch cultivation, indicating that the repeated batch cultivation of S. platensis is attractive and convenient.

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Section: Runsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 98%

Repeated batch cultivation of the microalga Spirulina platensis

Reinehr

Costa

2006

World J Microbiol Biotechnol

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“…These are dramatic changes in algae nutritional quality with changes in culture renewal rate, but their effect on rotifer lifespan was not determined in this work. Similar increases in protein content and decreases in carbohydrate content were observed when renewal rates of D. tertiolecta cultures were manipulated [31]. D. tertiolecta protein content more than doubled from 30 to 70% when culture renewal rate was increased from 10 to 50%.…”

Section: Dietary Restriction In Rotiferssupporting

confidence: 60%

Rotifers as models for the biology of aging

Snell

2014

Internat. Rev. Hydrobiol.

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It has been two decades since 1993 when research on the biology of rotifer aging was last reviewed by Enesco. Much has transpired during this time as rotifer biologists have adapted to the “omics” revolution and incorporated these techniques into the experimental analysis of rotifers. Rotifers are amenable to many of these approaches and getting adequate quantities of DNA, RNA, and protein from rotifers is not difficult. Analysis of rotifer genomes, transcriptomes, and proteomes is rapidly yielding candidate genes that likely regulate a variety of features of rotifer biology. Parallel developments in aging biology have recognized the limitations of standard animal models like worms and flies and that comparative aging research has essentially ignored a large fraction of animal phylogeny in the lophotrochozoans. As experimentally tractable members of this group, rotifers have attracted interest as models of aging. In this paper, I review advances over the past 20 years in the biology of aging in rotifers, with emphasis on the unique contributions of rotifer models for understanding aging. The majority of experimental work has manipulated rotifer diet and followed changes in survival and reproductive dynamics like mean lifespan, maximum lifespan, reproductive lifespan, and mortality rate doubling time. The main dietary manipulation has been some form of caloric restriction, withholding food for some period or feeding continuously at low levels. There have been comparative studies of several rotifer species, with some species responding to caloric restriction with life extension, but others not, at least under the tested food regimens. Other aspects of diet are less explored, like nutritional properties of different algae species and their capacity to extend rotifer lifespan. Several descriptive studies have reported many genes involved in rotifer aging by comparing gene expression in young and old individuals. Classes of genes up or down-regulated during aging have become prime targets for rotifer aging investigations. Alterations of gene expression by exposure to specific inhibitors or RNAi knockdown will probably yield valuable insights into the cellular mechanisms of rotifer life extension. I highlight major experimental contributions in each of these areas and indicate opportunities where I believe additional investigation is likely to be profitable.

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“…It is also reported that high pH leads to increased growth, CO 2 uptake and amino acid content in Skeletonema costatum (Taraldsvik and Myklestad, 2000). The factors affecting microalgae cultures include also dilution rate (Sukenik and Wahnon, 1991;Alonso et al, 2000) and culture medium (Fabregas et al, 1995;Sanchez et al, 2000). In the present study, three experiments were designed to find ways of improving Isochrysis affinis galbana (T-Iso) growth and reducing the production costs associated with labor, energy and chemical supplements, which are considered as the greatest expenses for hatcheries (Boeing, 2000).…”

Section: Introductionmentioning

confidence: 97%

Optimizing conditions for the continuous culture of Isochrysis affinis galbana relevant to commercial hatcheries

et al. 2012

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Optimal culture conditions were investigated for the continuous culture of Isochrysis affinis galbana (T-Iso) by assessing the effects of irradiance, temperature, pH and nitrogen and phosphorus requirements. Highlights ► We investigated optimal conditions for Isochrysis affinis galbana (T-Iso). ► Irradiance efficiency is optimal for a given set of irradiance and dilution rate. ► pH-temperature and pH-irradiance interactions occur on growth. ► We propose an integrative optimization procedure for T-Iso continuous cultures.

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Renewal rate and nutrient concentration as tools to modify productivity and biochemical composition of cyclostat cultures of the marine microalga Dunaliella tertiolecta

Cited by 11 publications

References 0 publications

Repeated batch cultivation of the microalga Spirulina platensis

Repeated batch cultivation of the microalga Spirulina platensis

Rotifers as models for the biology of aging

Optimizing conditions for the continuous culture of Isochrysis affinis galbana relevant to commercial hatcheries

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