Renin, Cyclooxygenase-2 and Neuronal Nitric Oxide Synthase in the Kidneys of Transgenic Tsukuba Hypertensive Mouse

Yabuki, Akira; Matsumoto, Mitsuharu; Kamimura, Ryotaro; Taniguchi, Kazuyuki; Suzuki, Syusaku

doi:10.1538/expanim.53.387

Cited by 3 publications

(6 citation statements)

References 14 publications

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“…Parietal layer of the glomerular capsule in male mouse kidney consisted of a single layer of simple cuboidal epithelium, whereas it was single layer of simple squamous epithelium in female. This finding is consistent with the observations of Barberini et al [1] and Yabuki et al [4,3,6]. Vacuoles of different sizes and shapes were observed in the proximal convoluted tubules (PCT) in male, but this histological feature was not detected in female kidney.…”

Section: Discussionsupporting

confidence: 92%

“…Sex-and strain-dependent differences in the morphology of glomerular capsule have been described in different laboratory animals [5,1,4,7]. DBA/2CrSlc mouse kidney also revealed sex-dependent kidney morphology in the present investigation.…”

Section: Discussionsupporting

confidence: 71%

“…Immunohistochemical study was conducted for the detection of renin, COX-2, and nNOS. The materials and methods for immunohistochemical study were followed based on the protocol supplied by the company and according to the report published previously by Yabuki et al [4]. A total count of glomeruli for both male and female mice was counted by using a light microscope connected with a digital camera (Nikon ECLIPSE E 600) and a computerized monitor (BUFFALO, Japan).…”

Section: Experimental Animalsmentioning

confidence: 99%

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Light Microscopic and Immunohistochemical Study Revealed Sex-Dependent Kidney Morphology in DBA/2crSlc Mice

Awal

2013

BBJ

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Aim: Sex-dependent differences in kidney histology have been observed in different species of the laboratory animals. The present study was conducted to evaluate the sex and strain-dependent changes in DBA/2CrSlc mouse kidney morphology by using light microscopy and immunohistochemistry. Methods: A total of 12 DBA/2CrSlc male and female mice of 2 months of age were used in this study. Mice were sacrificed by exsanguination under anesthesia using a mixture of Ketamine and Medetomidine. Both right and left kidneys were removed aseptically and central slices including hilum were cut perpendicular to the long axis of the organ and preserved in Zamboni solution. Paraffin blocks were made and tissue sections were stained with hematoxylin and eosin, and PAS stains to observe the general morphology of the kidney glomerulus. Immunohistochemistry was performed to detect renin positive

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 71%

Section: Experimental Animalsmentioning

confidence: 99%

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Light Microscopic and Immunohistochemical Study Revealed Sex-Dependent Kidney Morphology in DBA/2crSlc Mice

Awal

2013

BBJ

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“…In the normal human kidney, COX-2 is constitutively expressed in non-MD regions, including glomerular podocytes, small blood vessels, and papillary interstitial cells [13,18]. In contrast, the normal mouse kidney shows minimal expression in MD cells, in the presence or absence of immunopositive signals [10,30]. Although such species-based differences exist between humans and mice, COX-2 expression in MD cells of KK-A y mice closely resembles that of human DN.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Examination of Cyclooxygenase-2 and Renin in a KK-Ay Mouse Model of Diabetic Nephropathy

2010

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Abstract:The renin-angiotensin system plays a central role in the pathological mechanisms of diabetic nephropathy and is regulated by renal expression of cyclooxygenase-2 (COX-2). In the present study, the kidneys of diabetic KK-A y mice, a model of human type 2 diabetes, were investigated histologically and immunohistochemically at 8, 12, 16, and 20 weeks of age, and changes in renal lesions and expression of COX-2 and renin were evaluated quantitatively. Glomerular damage, characterized by expansion of mesangial matrices and nodular lesions, was observed in the kidneys of these mice. The glomerular sclerosis score gradually increased with age and was significantly higher than those of age-matched control C57BL/6 mice at 12, 16, and 20 weeks of age. Although mild tubulointerstitial damage was observed, there was no significant change in the interstitial fibrosis score. These findings were considered early diabetic nephropathy changes. COX-2-positive signals were consistently detected in the macula densa cells of the thick ascending limbs in all KK-A y mice, with a slightly higher score observed at 8 weeks of age. No COX-2-positive signals were detected in C57BL/6 mice. Renin-positive signals were commonly detected in the juxtaglomerular arterioles, and the scores in KK-A y mice increased at 16 weeks and decreased at 20 weeks of age. The present study demonstrated activation of renal COX-2 and renin expression in diabetic KK-A y mice at different stages. This finding suggests that these two enzymes contribute to the development and progression of diabetic nephropathy via different mechanisms.

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“…In THM mice, increased expression of COX-2 in the macula densa has been reported [13], and an important role for RAAS in cardiac hypertrophy has been noted [14]. In addition, activation of RAAS has been demonstrated in rats with heart failure [6].…”

Section: Introductionmentioning

confidence: 99%

Pulmonary and Cardiorenal Cyclooxygenase-1 (COX-1), -2 (COX-2), and Microsomal Prostaglandin E Synthase-1 (mPGES-1) and -2 (mPGES-2) Expression in a Hypertension Model

Radi

Ostroski

2007

Mediators of Inflammation

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Hypertensive mice that express the human renin and angiotensinogen genes are used as a model for human hypertension because they develop hypertension secondary to increased renin-angiotensin system activity. Our study investigated the cellular localization and distribution of COX-1, COX-2, mPGES-1, and mPGES-2 in organ tissues from a mouse model of human hypertension. Male (n = 15) and female (n = 15) double transgenic mice (h-Ang 204/1 h-Ren 9) were used in the study. Lung, kidney, and heart tissues were obtained from mice at necropsy and fixed in 10% neutral buffered formalin followed by embedding in paraffin wax. Cut sections were stained immunohistochemically with antibodies to COX-1, COX-2, mPGES-1, and mPGES-2 and analyzed by light microscopy. Renal expression of COX-1 was the highest in the distal convoluted tubules, cortical collecting ducts, and medullary collecting ducts; while proximal convoluted tubules lacked COX-1 expression. Bronchial and bronchiolar epithelial cells, alveolar macrophages, and cardiac vascular endothelial cells also had strong COX-1 expression, with other renal, pulmonary, or cardiac microanatomic locations having mild-to-moderate expression. mPGES-2 expression was strong in the bronchial and bronchiolar epithelial cells, mild to moderate in various renal microanatomic locations, and absent in cardiac tissues. COX-2 expression was strong in the proximal and distal convoluted tubules, alveolar macrophages, and bronchial and bronchiolar epithelial cells. Marked mPGES-1 was present only in bronchial and bronchiolar epithelial cells; while mild-to-moderate expression was present in other pulmonary, renal, or cardiac microanatomic locations. Expression of these molecules was similar between males and females. Our work suggests that in hypertensive mice, there are (a) significant microanatomic variations in the pulmonary, renal, and cardiac distribution and cellular localization of COX-1, COX-2, mPGES-1, and mPGES-2, and (b) no differences in expression between genders.

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Renin, Cyclooxygenase-2 and Neuronal Nitric Oxide Synthase in the Kidneys of Transgenic Tsukuba Hypertensive Mouse

Cited by 3 publications

References 14 publications

Light Microscopic and Immunohistochemical Study Revealed Sex-Dependent Kidney Morphology in DBA/2crSlc Mice

Light Microscopic and Immunohistochemical Study Revealed Sex-Dependent Kidney Morphology in DBA/2crSlc Mice

Immunohistochemical Examination of Cyclooxygenase-2 and Renin in a KK-Ay Mouse Model of Diabetic Nephropathy

Pulmonary and Cardiorenal Cyclooxygenase-1 (COX-1), -2 (COX-2), and Microsomal Prostaglandin E Synthase-1 (mPGES-1) and -2 (mPGES-2) Expression in a Hypertension Model

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