Renoprotective activity of anethole- rich fraction from aromatic herbs on junk food induced diabetic nephropathy in rats

Abstract

Purpose This study was carried out to study the effect of anethole rich fraction on the Diabetic Nephropathy (DN) rats, and explore the mechanisms. Methods Male wistar rats were grouped into 4 (n = 6 per group): Control, junk food fed DN induced rats, low dose and high dose groups. DN was induced by oral junk food feeding. They were monitored for blood glucose levels and urine protein content at regular intervals. At the end of the study, the biological and hematological parameters were measured. Pancreatic and kidney viscera were taken to observe histopathological changes. Results Both the doses of anethole rich fraction could drastically lower blood glucose levels, Low Density Lipoprotein (LDL), Glycated Serum Protein (GSP), Total Cholesterol (TC), Triglycerides (TG) (P < 0.01), Malondialdehyde (MDA) level (P < 0.01 or P < 0.05), increases insulin level (P < 0.01), High Density Lipoproteins (HDL), Glutathione Reductase (GSH) and Superoxide Dismutase (SOD) (P < 0.01 or P < 0.05 or P > 0.05). Both doses of anethole rich fraction also improved the pathological changes of kidney and pancreatic tissues in DN rats (P < 0.01 or P < 0.05 or P > 0.05). Conclusions Hence it can be hypothesized that the high dose of anethole rich fraction (100 mg/kg) could reach the intervention effect and could ameliorate renal damage in DN rats by improving the renal functions, oxidative stress levels, glycometabolism and pathological changes of pancreas and kidney.

Fennel Essential Oil as a Complementary Therapy in the Management of Diabetes

“…The positive effects of fennel products on diabetes are attributed to anethole, which is the quantitatively dominant component in fennel essential oil. Vellapandian et al have previously demonstrated that the administration of anethole-rich extracts can ameliorate renal damage in diabetic rats at a dose of 100 mg anethole/kg [ 23 , 24 ]. Similar results have been obtained by Samadi-Hoshahr et al, at a dose of 80 mg anethole/kg [ 25 ].…”