Renoprotective Effect of Platelet‐Rich Plasma on Cisplatin‐Induced Nephrotoxicity in Rats

Abstract

Platelet-rich plasma (PRP) has grown as an attractive biologic instrument in regenerative medicine for its powerful healing properties. It is considered as a source of growth factors that may induce tissue repairing and improve fibrosis. This product has proven its efficacy in multiple studies, but its effect on cisplatin-induced nephrotoxicity has not yet been elucidated. The present investigation was performed to estimate the protective impact of platelet-rich plasma against cisplatin- (CP-) evoked nephrotoxicity in male rats. Nephrotoxicity was induced in male Wistar rats by right uninephrectomy followed by CP administration. Uninephrectomized rats were assigned into four groups: (1) control group, (2) PRP group, (3) CP group, and (4) CP + PRP group. PRP was administered by subcapsular renal injection. Renal function, inflammatory cytokines, and growth factor level as well as histopathological investigation were carried out. Treatment with PRP attenuated the severity of CP-induced nephrotoxicity as evidenced by suppressed creatinine, blood urea nitrogen (BUN), and N-acetyl glucosaminidase (NAG) levels. Moreover, PRP depressed intercellular adhesion molecule-1 (ICAM-1), kidney injury molecule-1 (KIM-1), caspase-3, and transforming growth factor-beta 1 (TGF-β1) levels, while enhanced the epidermal growth factor (EGF) level. These biochemical results were reinforced by the histopathological investigation, which revealed restoration of normal renal tissue architectures. These findings highlight evidence for the possible protective effects of PRP in a rat model of CP-induced nephrotoxicity, suggesting a new avenue for using PRP to improve the therapeutic index of cisplatin.

Possible protective effect of platelet‐rich plasma on a model of cisplatin‐induced nephrotoxicity in rats: A light and transmission electron microscopic study

“…In addition, this study is consistent with a recent study by Salem, Helmi, and Assaf (). Although the work by Salem et al () support our results, there are some variances between the studies. In the current study, we evaluated the general morphology, the PAS reaction, the expression of caspase‐3, the ultrastructural, and the morphometrical changes.…”

“…In the current study, we evaluated the general morphology, the PAS reaction, the expression of caspase‐3, the ultrastructural, and the morphometrical changes. Salem et al () examined the influence of PRP on renal injury induced by Cis in a rat model with PRP administration. while, the renal function, inflammatory cytokines, and GF level were carried out.…”

“…In the current study, we evaluated the general morphology, the PAS reaction, the expression of caspase-3, the ultrastructural, and the morphometrical changes. Salem et al (2018)…”

Histological Study on the Possible Ameliorating Effect of Platelet Rich Plasma on Ischemia/Reperfusion Injury in Testicular Torsion Model in Adult Albino Rat

“…PRP a strong antioxidant has been reported to protect against ischemia-reperfusion injury in the brain, heart, and kidney. Furthermore, there is no adverse effect of PRP on male fertility [57] . Therefore, we attempted to use PRP for the treatment of testicular ischemia-reperfusion injury.…”

“…PRP therapeutic use in regenerative medicine has proved proper effects and safe in experimental animals and in the clinical treatment of human patients. Therefore, new research are needed, with greater accuracy and experimental improvement, using a larger numbers of patients, and the definition of a approval to establish more exactly the clinical conditions for which the PRP should be properly and precisely employed [8,57] .…”