Reo-like virus in white shrimp Penaeus van-namei (Crustacea: Decapoda): co-occurrence with Baculovirus penaei in experimental infections

Abstract

A virus, tentatively identified as reo-like, occurred concurrently with experimentallyinduced Baculovirus penaei (BP) infection in cultured white shrimp larvae Penaeus vannamei. Each shrimp with a reo-like viral infection also had a BP infection, but not all BP-infected shrimp had a reolike infection. Both viruses occurred in the same tissues and occasionally withln the same cell. The reolike virus developed in epithelia1 cells of the anterior midgut and in reserve-and fibrillar-cells of the hepatopancreas. The paraspherical and non-enveloped reo-like virions (ca 50 nm diam.) occurred as unordered aggregates in the cell cytoplasm. Their etiology has not been determined. Reo-like vinons may have been ~ntroduced along with the BP virus, or, were latent and only manifested due to stress induced by the more pathogenic BP virus.

Bioinformatics of Recent Aqua- and Orthoreovirus Isolates from Fish: Evolutionary Gain or Loss of FAST and Fiber Proteins and Taxonomic Implications

“…Shellfish isolates include ones from mollusks (oysters and clams) [53], [54] and crustaceans (crabs and shrimp) [55]–[57]. The prospect of there being such shellfish aquareoviruses is intriguing, but should perhaps be met with some skepticism regarding their natural hosts or taxonomy, since they suggest an unusually broad range of productive infection by viruses from a single genus in the absence of any vector/host relationships among the hosts.…”

Pre‐exposure to infectious hypodermal and haematopoietic necrosis virus or to inactivated white spot syndrome virus (WSSV) confers protection against WSSV in Penaeus vannamei (Boone) post‐larvae

“…This state, also called viral co‐infection, is characterized by the presence of two or more viruses in the same host, infecting the same tissue or cell (Harper 1986). Several investigations have revealed this condition in shrimp (Krol, Hawkins & Overstreet 1990; Madhavi, Janakiram, Jayasree & Murthy 2002; Manivannan, Otta, Karunasagar & Karunasagar 2002; Otta, Karunasagar & Karunasagar 2003; Umesha, Uma, Otta, Karunasagar & Karunasagar 2003; Flegel, Nielsen, Thamavit, Kongtim & Pasharawipas 2004).…”

Differentiation of BP-type baculovirus strains using in situ hybridization

“…Usually when the reaction is positive, the another Pacific strain of BP that is not morphologically different. Double infections by different viruses in the same specimen have already been reported in crustaceans for a baculovirus and + a reovirus (Bonami 1980, Mari 1987, Krol et al 1990). By contrast, double infections with 2 different strains from the same viral family have not been previously noted, probably due to the inability to distinguish different strains or to the lack of suitable tools to recognize them.…”