Reorganisation of a mesophilic biogas microbiome as response to a stepwise increase of ammonium nitrogen induced by poultry manure supply

Alsouleman, Khulud; Linke, Bernd; Klang, Johanna; Klocke, Michael; Krakat, Niclas; Theuerl, Susanne

doi:10.1016/j.biortech.2016.02.104

Cited by 40 publications

(48 citation statements)

References 16 publications

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“…This species is able to degrade fatty acids and aromatic acids in a syntrophic relationship with hydrogenotrophic methanogens [61]. This finding is in accordance with previous studies which showed a predominance of the hydrogenotrophic pathway of methane formation under elevated TAN concentration e.g., [14,15,38,62,63]. Hydrogenotrophic archaea are also known as hydrogen scavengers, meaning they depend on H 2 which is produced by their neighboring bacteria (such as members of the genera Syntophomonas, Syntrophobacter, Syntrophus, Propionibacter, Pelotomaculum, Smithella, or Clostridium [38,39]) who in turn can only grow if the H 2 is consumed.…”

Section: Bacterial Community At High Tan Concentrationsupporting

confidence: 92%

“…This genus is obligate acetoclastic; hence, the members of this genus only produce methane from acetic acid [22,66]. According to the assumption that this genus is abundant in well-performing reactor systems [14,15,67], the VFA concentrations in the reactors SB-M1 and M-SB1 were low while the biogas yields and methane contents were constant, indicating an undisturbed process. Therefore, it can be concluded that the bacterial communities which adapted to the exchanged feedstocks over the course of time were able to degrade the provided chemical compounds into acetic acid, while the archaeal community was not inhibited but could further degrade the intermediates into methane.…”

Section: Archaeal Communitymentioning

confidence: 99%

“…The development of the microbial community depends on the available substrates. Different feedstocks with different chemical compositions and bioavailability lead to different microbial communities [11,[13][14][15][16][17][18][19]. Therefore, knowledge of the microbial reaction to different feedstock compositions is highly important in order to avoid process disturbances, and changes in the feedstock composition have to be performed with caution.…”

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confidence: 99%

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Effect of a Profound Feedstock Change on the Structure and Performance of Biogas Microbiomes

et al. 2020

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In this study the response of biogas-producing microbiomes to a profound feedstock change was investigated. The microbiomes were adapted to the digestion of either 100% sugar beet, maize silage, or of the silages with elevated amounts of total ammonium nitrogen (TAN) by adding ammonium carbonate or animal manure. The feedstock exchange resulted in a short-range decrease or increase in the biogas yields according to the level of chemical feedstock complexity. Fifteen taxa were found in all reactors and can be considered as generalists. Thirteen taxa were detected in the reactors operated with low TAN and six in the reactors with high TAN concentration. Taxa assigned to the phylum Bacteroidetes and to the order Spirochaetales increased with the exchange to sugar beet silage, indicating an affinity to easily degradable compounds. The recorded TAN-sensitive taxa (phylum Cloacimonetes) showed no specific affinity to maize or sugar beet silage. The archaeal community remained unchanged. The reported findings showed a smooth adaptation of the microbial communities, without a profound negative impact on the overall biogas production indicating that the two feedstocks, sugar beet and maize silage, potentially do not contain chemical compounds that are difficult to handle during anaerobic digestion.

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Section: Bacterial Community At High Tan Concentrationsupporting

confidence: 92%

Section: Archaeal Communitymentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of a Profound Feedstock Change on the Structure and Performance of Biogas Microbiomes

et al. 2020

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“…Some studies have also reported that acetotrophic methanogens are more susceptible to stress conditions (such as high-level ammonium, low pH, high hydrogen partial pressure etc.) than hydrogenotrophic ones and the acetoclastic methanogenesis is replaced by H 2 -dependent methanogenesis under stress conditions [14,45]. To date, there are a restricted number of syntrophic acetate-oxidizing bacteria (SAOB) have been identified, Clostridium ultunense, Syntro-phaceticus schinkii, Tepidanaerobacter acetatoxydans, Thermacetogenium phaeum and Thermotoga lettingae [46].…”

Section: Succession Of Dominant Microorganisms Involved In the Propiomentioning

confidence: 99%

Functional bacterial and archaeal dynamics at slightly acidic conditions revealed by pyrosequencing in a propionate-fed UASB reactor

Ban¹,

Zhang²,

Li³

2019

Desalination and Water Treatment

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a b s t r a c tTo reveal the response mechanism of syntrophic propionate degrading communities to slightly acidic environments, the performance and microbial community structure at pH 7.0, 6.0 and 5.0 in a propionate-fed upflow anaerobic sludge blanket (UASB) reactor were investigated. The propionate removal and the specific methane production rate were reduced by 33.9%-69.5% and 10.9%-18.9% independently as pH decreased to 5.0 from 7.0. Pyrosequencing based on 16S rRNA gene showed that the microbial diversity in the UASB reactor was increased as pH decreased. Syntrophobacter was the dominant propionate-oxidizing bacteria (POB) at pH 7.0 and its relative abundance reached 23.0%. When the pH decreased from 7.0 to 5.0 in stages, the dominant POB were replaced by Smithella and Pelotomaculum. The hydrogenotrophic and acetotrophic methanogens were dominated by Methanospirillum and Methanosaeta at pH 7.0. pH decrease resulted in the relative abundance of acetotrophic methanogens decreased by 88.6% and hydrogenotrophic methanogens became the major methanogens present. The relative abundance of Methanobacterium was up to 5.0% at pH 5.0. pH decrease broke the normal metabolic network, and then further exacerbated the instability of process. These results reveal the microbial mechanism of process instability caused by pH reduction and provide guidance for the microbial management of anaerobic digesters.

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“…It was important to consider shifting to variable extant KS values or explicitly modeling the effects of diffusion during the treatment processes. Alsouleman (2015) investigated an anaerobic digestion experiment to evaluate the impact of increasing amounts of ammonium nitrogen due to poultry manure addition on the reactor performance, especially on the microbiome response. The results showed that the addition of 50% poultry manure led to a process disturbance indicated by a high VFA content (almost 10 gHAc-Eq L -1 ) in combination with elevated concentrations of ammonium nitrogen (5.9 NH4 + -N kg FM -1 ) and free ammonia (0.5 g NH3 kgFM -1 ).…”

Section: Anaerobic Process For Organic Wastementioning

confidence: 99%

[ARTICLE WITHDRAWN] Anaerobic Process

Yang

Mei

et al. 2016

Water Environment Research

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Reorganisation of a mesophilic biogas microbiome as response to a stepwise increase of ammonium nitrogen induced by poultry manure supply

Cited by 40 publications

References 16 publications

Effect of a Profound Feedstock Change on the Structure and Performance of Biogas Microbiomes

Effect of a Profound Feedstock Change on the Structure and Performance of Biogas Microbiomes

Functional bacterial and archaeal dynamics at slightly acidic conditions revealed by pyrosequencing in a propionate-fed UASB reactor

[ARTICLE WITHDRAWN] Anaerobic Process

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