Reorganization of Acyl Flux through the Lipid Metabolic Network in Oil-Accumulating Tobacco Leaves

Zhou, Xue-Rong; Bhandari, Sajina; Johnson, Brandon S.; Kotapati, Hari Kiran; Allen, Doug K.; Vanhercke, Thomas; Bates, Philip D.

doi:10.1104/pp.19.00667

Cited by 26 publications

(22 citation statements)

References 71 publications

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“…Efforts to engineer increased production and accumulation of TAG in both oilseeds and vegetative tissues for more energy-dense biomass have achieved the most success through the combined targeting of multiple aspects of developmental central carbon metabolism to fatty acid synthesis (‘Push’), the incorporation of those fatty acids into TAG biosynthesis (‘Pull’), and stabilization of lipid droplets and minimization of TAG turnover (‘Package and Protect’) ( Beechey-Gradwell et al, 2019 ; Durrett et al, 2008 ; Parajuli et al, 2020 ; Slocombe et al, 2009 ; Vanhercke et al, 2014b , 2019b ; Weselake, 2016 ; Xu and Shanklin, 2016 ), with synergistic increases in TAG yields in vegetative tissues often observed when multiple genes are simultaneously targeted ( Kelly et al, 2013 ; Vanhercke et al, 2013 ; Winichayakul et al, 2013 ; Zale et al, 2016 ). The highest elevated TAG yields in leaves reported thus far (reviewed in Vanhercke et al, 2019b ) have been achieved in engineered tobacco lines ( Andrianov et al, 2010 ; Cai et al, 2019 ; Vanhercke et al, 2014a , 2017 ; Zhou et al, 2020 ), with some high lipid-producing (HLP) lines producing over 30% foliar TAG (DW) without drastic consequences on plant growth. Metabolic explanations for carbon partitioning in these lines, however, are incomplete, are not quantitative or mechanistic, and do not indicate why engineering efforts are more successful in certain plant species compared to others.…”

Section: Introductionmentioning

confidence: 99%

Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves

Chu

Koley

Jenkins

et al. 2022

Metabolic Engineering

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Section: Introductionmentioning

confidence: 99%

Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves

Chu

Koley

Jenkins

et al. 2022

Metabolic Engineering

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“…It has also been reported that several transcription factors, such as LEC1 , LEC2 , and FUS3 , are associated with embryo development and seed oil biosynthesis ( Chen et al, 2014 ; Zhang M. et al, 2016 ). However, many genes, proteins, regulatory factors, and multiple pathways are associated with the processes of tobacco seed oil biosynthesis and accumulation ( Figure 3 ), and the molecular mechanisms of fatty acid and TAG biosyntheses are not fully understood ( Zhang M. et al, 2016 ; Zhou et al, 2019 ).…”

Section: Role Of Biotechnology In Biodiesel Production In Tobacco Seed Oilmentioning

confidence: 99%

Advancements in Tobacco (Nicotiana tabacum L.) Seed Oils for Biodiesel Production

Wu¹,

Chuanchuan

Pan

et al. 2022

Front. Chem.

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With the increasing demand for fossil fuels, decreasing fossil fuel reserves and deteriorating global environment, humanity urgently need to explore new clean and renewable energy to replace fossil fuel resources. Biodiesel, as an environmentally friendly fuel that has attracted considerable attention because of its renewable, biodegradable, and non-toxic superiority, seems to be a solution for future fuel production. Tobacco (Nicotiana tabacum L.), an industrial crop, is traditionally used for manufacturing cigarettes. More importantly, tobacco seed is also widely being deemed as a typical inedible oilseed crop for the production of second-generation biodiesel. Advancements in raw material and enhanced production methods are currently needed for the large-scale and sustainable production of biodiesel. To this end, this study reviews various aspects of extraction and transesterification methods, genetic and agricultural modification, and properties and application of tobacco biodiesel, while discussing the key problems in tobacco biodiesel production and application. Besides, the proposals of new ways or methods for producing biodiesel from tobacco crops are presented. Based on this review, we anticipate that this can further promote the development and application of biodiesel from tobacco seed oil by increasing the availability and reducing the costs of extraction, transesterification, and purification methods, cultivating new varieties or transgenic lines with high oilseed contents, formulating scientific agricultural norms and policies, and improving the environmental properties of biodiesel.

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“…RuBisCO is one of the key targets because it is highly abundant in green biomass and expendable at least in the days immediately before harvest, when the plant has already accumulated sufficient energy reserves (Robert et al 2015). The provision of energy reserves can be re-enforced by genetic engineering, as shown for lipid accumulation in tobacco leaves (Zhou et al 2020). Some experiments even suggest that a 25% reduction in RuBisCO content can increase biomass accumulation by 10% if the carbon dioxide partial pressure is increased to The protein content is often measured in terms of total soluble protein and thus depends on the selected extraction conditions 120 Pa (Kanno et al 2017).…”

Section: Modulating Hcp Expression To Simplify Dsp and Increase Synthesis Capacitymentioning

confidence: 99%

Targeted genome editing of plants and plant cells for biomanufacturing

2021

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Plants have provided humans with useful products since antiquity, but in the last 30 years they have also been developed as production platforms for small molecules and recombinant proteins. This initially niche area has blossomed with the growth of the global bioeconomy, and now includes chemical building blocks, polymers and renewable energy. All these applications can be described as “plant molecular farming” (PMF). Despite its potential to increase the sustainability of biologics manufacturing, PMF has yet to be embraced broadly by industry. This reflects a combination of regulatory uncertainty, limited information on process cost structures, and the absence of trained staff and suitable manufacturing capacity. However, the limited adaptation of plants and plant cells to the requirements of industry-scale manufacturing is an equally important hurdle. For example, the targeted genetic manipulation of yeast has been common practice since the 1980s, whereas reliable site-directed mutagenesis in most plants has only become available with the advent of CRISPR/Cas9 and similar genome editing technologies since around 2010. Here we summarize the applications of new genetic engineering technologies to improve plants as biomanufacturing platforms. We start by identifying current bottlenecks in manufacturing, then illustrate the progress that has already been made and discuss the potential for improvement at the molecular, cellular and organism levels. We discuss the effects of metabolic optimization, adaptation of the endomembrane system, modified glycosylation profiles, programmable growth and senescence, protease inactivation, and the expression of enzymes that promote biodegradation. We outline strategies to achieve these modifications by targeted gene modification, considering case-by-case examples of individual improvements and the combined modifications needed to generate a new general-purpose “chassis” for PMF.

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Reorganization of Acyl Flux through the Lipid Metabolic Network in Oil-Accumulating Tobacco Leaves

Cited by 26 publications

References 71 publications

Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves

Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves

Advancements in Tobacco (Nicotiana tabacum L.) Seed Oils for Biodiesel Production

Targeted genome editing of plants and plant cells for biomanufacturing

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