Reorganization of endothelial cells cytoskeleton during formation of functional monolayer in vitro

Shakhov, Anton S.; Verin, Alexander D.; Alieva, Irina B.

doi:10.1134/s1990519x14020096

Cited by 10 publications

(6 citation statements)

References 50 publications

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“…Cytoskeletons of glomerular endothelial cells comprise mainly F-actin and affect the morphology and contractility of cells directly252627. Our data showed that GEnCs in the static culture were polygonal and had few F-actin microfilaments, which traversed the cell body at random and in multiple directions (though some stress fibers were at the cell periphery).…”

Section: Resultsmentioning

confidence: 74%

Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy

Zhou

Zhang

et al. 2016

Sci Rep

111

118

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Glomerular hypertension is an important factor exacerbating glomerular diseases to end-stage renal diseases because, ultimately, it results in glomerular sclerosis (especially in hypertensive and diabetic nephropathy). The precise mechanism of glomerular sclerosis caused by glomerular hypertension is unclear, due partly to the absence of suitable in vitro or in vivo models capable of mimicking and regulating the complex mechanical forces and/or organ-level disease processes. We developed a “glomerulus-on-a-chip” (GC) microfluidic device. This device reconstitutes the glomerulus with organ-level glomerular functions to create a disease model-on-a chip that mimics hypertensive nephropathy in humans. It comprises two channels lined by closely opposed layers of glomerular endothelial cells and podocytes that experience fluid flow of physiological conditions to mimic the glomerular microenvironment in vivo. Our results revealed that glomerular mechanical forces have a crucial role in cellular cytoskeletal rearrangement as well as the damage to cells and their junctions that leads to increased glomerular leakage observed in hypertensive nephropathy. Results also showed that the GC could readily and flexibly meet the demands of a renal-disease model. The GC could provide drug screening and toxicology testing, and create potential new personalized and accurate therapeutic platforms for glomerular disease.

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Section: Resultsmentioning

confidence: 74%

Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy

Zhou

Zhang

et al. 2016

Sci Rep

111

118

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“…43 This may explain why escin prevents pathological increases in blood vessel permeability in which reorganization of cytoskeleton is emerging. 44 , 45 Moreover, in primary isolated umbilical veins, Bougelet et al showed that escin (100–750 μg/mL), was able to reduce the hypoxia-induced endothelial damage as well as the formation of superoxide anions and leukotriene B4. 46 Therefore, in hypoxic condition causing disruption and reorganization of endothelial cytoskeleton, escin may have the capability to limit the vascular damage, thereby limiting vascular extravasation and resulting edema formation.…”

Section: Glucocorticoids and Glucocorticoid-like Activity Of Escinmentioning

confidence: 99%

Glucocorticoid-Like Activity of Escin: A New Mechanism for an Old Drug

Gallelli¹,

Cione²,

Wang³

et al. 2021

DDDT

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Saponins are a group of compounds used in clinical practice in the management of several diseases. Escin is a natural mixture of triterpene saponins which mainly consist of several isoforms, in which the α- and β-escin are predominant. β-escin is the major active compound that exerts a therapeutic effect by relieving tissue edema, promoting venous drainage, and reducing inflammation. In this review, we describe the features of its glucocorticoid-like activity that could explain its clinical effects. Using PubMed, Embase Cochrane library and reference lists for articles published until October 01, 2020, we documented that escin is likely able to exert its anti-inflammatory and anti-edematous effects through a glucocorticoid-like activity, but without the development of glucocorticoid-like adverse drug reactions.

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“…The rearrangements of the EC cytoskeleton and adhesives change cell shape (Ref. 26). Targosz-Korecka et al .…”

Section: Hyperglycaemia As the Main Cause Of Endothelial Dysfunction mentioning

confidence: 99%

“…The rearrangements of the EC cytoskeleton and adhesives change cell shape (Ref. 26). Targosz-Korecka et al showed that long-lasting hyperglycaemic conditions result in cytoskeletal changes such as stress fibre formation and F-actin polymerisation.…”

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confidence: 99%

Role of the Wnt signalling pathway in the development of endothelial disorders in response to hyperglycaemia

Durak-Kozica

Paszek

Stępień

2019

Expert Rev. Mol. Med.

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Introduction Diabetes mellitus (DM) is the most common metabolic disease. A WHO report from 2016 indicates that 422 million people worldwide suffer from DM or hyperglycaemia because of impaired glucose metabolism. Chronic hyperglycaemia leads to micro- and macrovessel damage, which may result in life-threatening complications. The Wnt pathway regulates cell proliferation and survival by modulating the expression of genes that control cell differentiation. Three linked Wnt pathways have been discovered thus far: a β-catenin-dependent pathway and two pathways independent of β-catenin – the planar cell polarity pathway and calcium-dependent pathway. The Wnt pathway regulates genes associated with inflammation, cell cycle, angiogenesis, fibrinolysis and other molecular processes. Areas covered This review presents the current state of knowledge regarding the contribution of the Wnt pathway to endothelial ageing under hyperglycaemic conditions and provides new insights into the molecular basis of diabetic endothelial dysfunction. Conclusion The β-catenin-dependent pathway is a potential target in the prophylaxis and treatment of early-stage diabetes-related vascular complications. However, the underlying molecular mechanisms remain largely undetermined and require further investigation.

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Reorganization of endothelial cells cytoskeleton during formation of functional monolayer in vitro

Cited by 10 publications

References 50 publications

Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy

Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy

Glucocorticoid-Like Activity of Escin: A New Mechanism for an Old Drug

Role of the Wnt signalling pathway in the development of endothelial disorders in response to hyperglycaemia

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