Reoxygenation speed and its implication for cellular injury responses in hypoxic RAW 264.7 cells

Lee, Jae Hyuk; Kim, Kyuseok; Jo, You Hwan; Hwang, Ji Eun; Chung, Hea Jin; Yang, Chungmi

doi:10.1016/j.jss.2017.11.005

Cited by 2 publications

(2 citation statements)

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“…Our findings are not consistent with current literature ( Lee et al, 2017 ; Srinivasan and Avadhani, 2007 ). For instance, when RAW 264.7 cells were incubated in hypoxic conditions (1% O 2 ) for 20 h, there was a significant increase in ROS production when compared to the cell incubated in normoxic conditions ( Lee et al, 2017 ) and when RAW 264.7 cells were grown under hypoxia (0.5% O 2 ) for 10 h, there was a significant increase in ROS production when compared to the cells grown under normoxia ( Srinivasan and Avadhani, 2007 ). Consequently, differences in exposure time and oxygen concentrations may have affected the production of ROS.…”

Section: Resultscontrasting

confidence: 99%

“…It was reported that the formation of ROS depends on constant cycles of anoxia and reoxygenation ( Dawson et al, 1993 ). Another study repeated that reoxygenation increased the formation of ROS in RAW 264.7 cell with fast reoxygenation producing more ROS when compared to slow reoxygenation ( Lee et al, 2017 ). In our study, we have seen a decrease in ROS when the cells were exposed to hypoxia and it may be due to exposing the cells to hypoxia for a longer period of time where no more available oxygen was present to produce ROS.…”

Section: Resultsmentioning

confidence: 97%

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Intra-abdominal infection (IAI) is a common and important cause of infectious mortality in intensive care units. Adequate source control and appropriate antimicrobial regimens are key in the management of IAI. In community-acquired IAI, guidelines recommend the use of different antimicrobial regimens according to severity. However, the evidence for this is weak. We investigated the effect of enterococcal coverage in antimicrobial regimens in a severe polymicrobial IAI model. We investigated the effects of imipenem/cilastatin (IMP) and ceftriaxone with metronidazole (CTX+M) in a rat model of severe IAI. We observed the survival rate and bacterial clearance rate. We identified the bacteria in blood culture. We measured lactate, alanine aminotransferase (ALT), creatinine, interleukin (IL)-6, IL-10, and reactive oxygen species (ROS) in the blood. Endotoxin tolerance of peripheral blood mononuclear cells (PBMCs) was also estimated to determine the level of immune suppression. In the severe IAI model, IMP improved survival and bacterial clearance compared to CTX+M. Enterococcus spp. were more frequently isolated in the CTX+M group. IMP also decreased plasma lactate, cytokine, and ROS levels. ALT and creatinine levels were lower in IMP group. In the mild-to-moderate IAI model, however, there was no survival difference between the groups. Immune suppression of PBMCs was observed in IAI model, and it was more prominent in the severe IAI model. Compared to CTX+M, IMP improved the outcome of rats in severe IAI model.

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Reoxygenation speed and its implication for cellular injury responses in hypoxic RAW 264.7 cells

Cited by 2 publications

References 26 publications

Bovine milk exosomes affected proliferation of macrophages under hypoxia

Bovine milk exosomes affected proliferation of macrophages under hypoxia

The Need of Enterococcal Coverage in Severe Intra-Abdominal Infection: Evidence from Animal Study

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