Repair of Alveolar Bone Defects with Osteogenic Polypeptide Modified HA_CF_PEEK Dental Implants

Abstract

The implant implanted into the body directly interacts with the bone tissue around the implant through its surface. The surface properties of the implant can affect the adhesion, proliferation, and differentiation of surrounding cells and ultimately determine the quality of osseointegration and the effect of implantation surgery. In this project, nano-hydroxyapatite (n-HA) and carbon fiber (CF) were added into the matrix, poly(ether-ether-ketone) (PEEK), forming the composite material HA/CF/PEEK. Since biologically active proteins and peptides can promote cell viability and proliferation, the osteogenic polypeptide sequence BEE1 was polymerized and grafted onto the surface of HA/CF/PEEK composites to generate HA/CF/PEEK-DPP and HA/CF/PEEK-DPP-BEE1 composites. The above materials were tested for physical characterization and in vitro cell activity by establishing a Beagle dog dentition defect model. HA/CF/PEEK type composites were selected for peri-implant bone defect repair and compared with titanium mesh alone or Bio-Gide membrane for alveolar bone defect repair. In the experiment, the HA/CF/PEEK material modified with osteogenic polypeptides changed in element composition, hydrophilicity, and surface morphology. in vitro cell assays detected that the surface of HA/CF/PEEK material modified with osteogenic polypeptides was more conducive to cell adhesion and proliferation and had no cytotoxicity. The peri-implant defect repair test showed that the HA/CF/PEEK-DPP-BEE1 composite material for alveolar bone repair in experimental dogs was better than that of titanium mesh or Bio-Gide membrane. Meanwhile, the mineralization speed and histomorphometry test at the bone defect further proved that this material could better promote bone neogenesis at the defect. Mineralization.