Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model

Cui, Lei; Liang, Bo; Liu, Guangpeng; Zhang, Wenjie; Chen, Lian; Sun, Jian; Yin, Shuo; Liu, Wei; Cao, Yilin

doi:10.1016/j.biomaterials.2007.08.042

Cited by 268 publications

(167 citation statements)

References 40 publications

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“…OM containing AsA2-P, Dex and β-GP is a cost-effective osteo-inducer of hASCs in vitro as well as in vivo. In several in vivo studies, the osteogenic commitment of ASCs has been enhanced by OM during the in vitro culture prior to implantation (Cui et al, 2007;Di Bella et al, 2008;Bohnenblust et al, 2009;Schubert et al, 2011). For clinical bone tissue engineering it is critical to reduce the total time required to regenerate functional bone tissue.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic medium is superior to growth factors in differentiation of human adipose stem cells towards bone-forming cells in 3D culture

Tirkkonen¹,

Haimi²,

Huttunen³

et al. 2013

eCM

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Human adipose stem cells (hASCs) have been recently used to treat bone defects in clinical practice. Yet there is a need for more optimal scaffolds and cost-effective approaches to induce osteogenic differentiation of hASCs. Therefore, we compared the efficiency of bone morphogenetic proteins (BMP-2 and BMP-7), vascular endothelial growth factor (VEGF), and osteogenic medium (OM) for the osteoinduction of hASCs in 3D culture. In addition, growth factors were tested in combination with OM. Commercially available bioactive glass scaffolds (BioRestore) and biphasic calcium phosphate granules (BoneCeramic) were evaluated as prospective carriers for hASCs. Both biomaterials supported hASC-viability, but BioRestore resulted in higher cell number than BoneCeramic, whereas BoneCeramic supported more significant collagen production. The most efficient osteo-induction was achieved with plain OM, promoting higher alkaline phosphatase activity and collagen production than growth factors. In fact, treatment with BMP-2 or VEGF did not increase osteogenic differentiation or cell number significantly more than maintenance medium with either biomaterial. Moreover, BMP-7 treatment consistently inhibited proliferation and osteogenic differentiation of hASCs. Interestingly, there was no benefit from growth factors added to OM. This is the first study to demonstrate that OM enhances hASCdifferentiation towards bone-forming cells significantly more than growth factors in 3D culture.

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Section: Discussionmentioning

confidence: 99%

Osteogenic medium is superior to growth factors in differentiation of human adipose stem cells towards bone-forming cells in 3D culture

Tirkkonen¹,

Haimi²,

Huttunen³

et al. 2013

eCM

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“…[44][45][46][47][48][49][50][51][52] Combinations of coral graft, BMPs and osteoprogenitor cells have shown potent bone healing potential which was comparable to the autologous bone grafting. 44,45,46,48,49 The cellular component of the composite graft originate in the vast majority from bone marrow. However, osteogenically induced adipose tissue stem cells have been utilized with favorable results.…”

Section: Animal Studiesmentioning

confidence: 99%

Is there a role of coral bone substitutes in bone repair?

Pountos

Giannoudis

2016

Injury

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Xenogeneic bone graft materials are an alternative to autologous bone grafting. Among such implants, coralline-derived bone grafts substitutes have a long track record as safe, biocompatible and osteoconductive graft materials. In this review, we present the available literature surrounding their use with special focus on the commercially available graft materials. Corals thanks to their chemical and structural characteristics similar to those of the human cancellous bone have shown great potential but clinical data presented to date is ambiguous with both positive and negative outcomes reported.Correct formulation and design of the graft to ensure adequate osteo-activity and resorption appears intrinsic to a successful outcome.

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“…Stony corals secrete an external calcium carbonate skeleton, mainly in the form of aragonite with some calcite (Abramovitch- showed similar results in vitro with adipose-derived stem cells from dogs (Cui et al, 2007). In vivo, an early study reported successful healing of cortical and cancellous, non-critical and critical sized bone defects in dogs using four different corals; Porites, Goniopora, Favites or Lobophyllia (Guillemin et al, 1987) and Gao et al reported superior healing of a segmental tibial defect in sheep with coral compared to tricalcium phosphate (Ohgushi, 1997).…”

Section: Coralmentioning

confidence: 87%

Designs from the deep: Marine organisms for bone tissue engineering

Clarke

Walsh

Maggs

et al. 2011

Biotechnology Advances

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Current strategies for bone repair have accepted limitations and the search for synthetic graft materials or for scaffolds that will support ex vivo bone tissue engineering continues. Biomimetic strategies have led to the investigation of naturally occurring porous structures as templates for bone growth. The marine environment is rich in mineralizing organisms with porous structures, some of which are currently being used as bone graft materials and others that are in early stages of development. This review describes the current evidence available for these organisms, considers the relative promise of each and suggests potential future directions.

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Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model

Cited by 268 publications

References 40 publications

Osteogenic medium is superior to growth factors in differentiation of human adipose stem cells towards bone-forming cells in 3D culture

Osteogenic medium is superior to growth factors in differentiation of human adipose stem cells towards bone-forming cells in 3D culture

Is there a role of coral bone substitutes in bone repair?

Designs from the deep: Marine organisms for bone tissue engineering

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