Repair of DNA Dewar Photoproduct to (6-4) Photoproduct in (6-4) Photolyase

Ai, Yuejie; Liao, Rong‐Zhen; Chen, Shi-Lu; Hua, Weijie; Fang, Wei‐Hai; Luo, Yi

doi:10.1021/jp204128k

Cited by 13 publications

(15 citation statements)

References 38 publications

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“…Photolyases reverting 64PPs have been described in a series of eukaryotic cells in addition to those handling CPDs (Essen and Klar, ; Sancar, ). Interestingly, biochemical studies showed that these 64PPs photolyases may also repair DEWs but with little efficiency compared with 64PPs (Yamamoto et al ., ; Glas et al ., ; ; Ai et al ., ; ; Fingerhut et al ., ). In addition, TT DEW was found to be reluctant to photoreversion.…”

Section: Discussionmentioning

confidence: 99%

The significance of the Dewar valence photoisomer as a UV radiation‐induced DNA photoproduct in marine microbial communities

Meador

Baldwin

Pakulski

et al. 2014

Environmental Microbiology

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Induction of pyrimidine dimers in DNA by solar UV radiation has drastic effects on microorganisms. To better define the nature of these DNA photoproducts in marine bacterioplankton and eukaryotes, a study was performed during a cruise along a latitudinal transect in the Pacific Ocean. The frequency of all possible cyclobutane pyrimidine dimers, pyrimidine (6-4) pyrimidone photoproducts (64PPs) and their related Dewar valence isomers (DEWs) was determined by high-performance liquid chromatography-mass spectrometry. Studied samples were bacterioplankton and eukaryotic fractions isolated from sea water either collected before sunrise or exposed to ambient sunlight from sunrise to sunset. Isolated DNA dosimeters were also exposed to daily sunlight for comparison purposes. A first major result was the observation in all samples of large amounts of DEWs, a class of photoproducts rarely considered outside photochemical studies. Evidence was obtained for a major role of UVA in the formation of these photoisomerization products of 64PPs. Considerations on the ratio between the different classes of photoproducts in basal and induced DNA damage suggests that photoenzymatic repair (PER) is an important DNA repair mechanism used by marine microorganisms occupying surface seawater in the open ocean. This result emphasizes the biological role of DEWs which are very poor substrate for PER.

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Section: Discussionmentioning

confidence: 99%

The significance of the Dewar valence photoisomer as a UV radiation‐induced DNA photoproduct in marine microbial communities

Meador

Baldwin

Pakulski

et al. 2014

Environmental Microbiology

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“…Concerning the reaction mechanism of repair of the T(Dew)C lesion by (6‐4) PL, theoretical and experimental studies agree that that the first step is reversion of the Dewar PP to a (6‐4) PP upon electron injection by photoexcited FADH − . The (6‐4) PP would subsequently be repaired as discussed above.…”

Section: Repair Of Dewar Photoproductsmentioning

confidence: 85%

“…With respect to the inability of (6‐4) PL to repair the T(Dew)T lesion , this cannot be attributed to poor substrate binding, as a high affinity for T(Dew)T‐containing substrates has been demonstrated . Rather, reversion of the T(Dew)T to the T(6‐4)T lesion appears to be inhibited, either by an unfavorable electron transfer from photoexcited FADH − to T(Dew)T or by a higher activation barrier for the splitting of the N3′‐C6′ bond in the anion radical of the T(Dew)T lesion (compared to T(Dew)C) .…”

Section: Repair Of Dewar Photoproductsmentioning

confidence: 99%

Repair of (6‐4) Lesions in DNA by (6‐4) Photolyase: 20 Years of Quest for the Photoreaction Mechanism

Yamamoto

Plaza

Brettel

2017

Photochem & Photobiology

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Exposure of DNA to ultraviolet (UV) light from the Sun or from other sources causes the formation of harmful and carcinogenic crosslinks between adjacent pyrimidine nucleobases, namely cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts. Nature has developed unique flavoenzymes, called DNA photolyases, that utilize blue light, that is photons of lower energy than those of the damaging light, to repair these lesions. In this review, we focus on the chemically challenging repair of the (6-4) photoproducts by (6-4) photolyase and describe the major events along the quest for the reaction mechanisms, over the 20 years since the discovery of (6-4) photolyase.

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“…The fact that plant cells can effectively repair selected types of UV-induced DNA lesions via photoreactivation has been well documented. According to our knowledge, the activity of this repair system in plants is limited to the repair of CPDs, 6-4 PPs and Dewar photoproducts only [14][15][16]. Photoreactivation is performed by photolyases which use UV-A/blue light energy to simply reverse pyrimidine dimers formed under UV.…”

Section: Nucleotide Excision Repairmentioning

confidence: 99%

The Dark Side of UV-Induced DNA Lesion Repair

Strzałka

Zgłobicki

Kowalska

et al. 2020

Genes

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In their life cycle, plants are exposed to various unfavorable environmental factors including ultraviolet (UV) radiation emitted by the Sun. UV-A and UV-B, which are partially absorbed by the ozone layer, reach the surface of the Earth causing harmful effects among the others on plant genetic material. The energy of UV light is sufficient to induce mutations in DNA. Some examples of DNA damage induced by UV are pyrimidine dimers, oxidized nucleotides as well as single and double-strand breaks. When exposed to light, plants can repair major UV-induced DNA lesions, i.e., pyrimidine dimers using photoreactivation. However, this highly efficient light-dependent DNA repair system is ineffective in dim light or at night. Moreover, it is helpless when it comes to the repair of DNA lesions other than pyrimidine dimers. In this review, we have focused on how plants cope with deleterious DNA damage that cannot be repaired by photoreactivation. The current understanding of light-independent mechanisms, classified as dark DNA repair, indispensable for the maintenance of plant genetic material integrity has been presented.

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Repair of DNA Dewar Photoproduct to (6-4) Photoproduct in (6-4) Photolyase

Cited by 13 publications

References 38 publications

The significance of the Dewar valence photoisomer as a UV radiation‐induced DNA photoproduct in marine microbial communities

The significance of the Dewar valence photoisomer as a UV radiation‐induced DNA photoproduct in marine microbial communities

Repair of (6‐4) Lesions in DNA by (6‐4) Photolyase: 20 Years of Quest for the Photoreaction Mechanism

The Dark Side of UV-Induced DNA Lesion Repair

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