Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs

Pei, Ming; He, Fan; Boyce, Brandon M.; Kish, Vincent

doi:10.1016/j.joca.2008.11.017

Cited by 127 publications

(87 citation statements)

References 43 publications

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“…The superior cartilage bonding to adjacent native tissue seen in the 6-month triphasic group was a notable finding given the well-known issue of incomplete cartilage bonding in osteochondral autografts. 10−12 It further demonstrates the capacity of MSCs to differentiate into various tissues 38 and the ability of MSCs to achieve good bonding to surrounding native cartilage. 39 Additional longer-term studies are required here to chart the course of repair provided by these implants.…”

Section: Journal Of Orthopaedic Research December 2010mentioning

confidence: 98%

A novel MSC‐seeded triphasic construct for the repair of osteochondral defects

Marquaß

Somerson

Hepp

et al. 2010

Journal Orthopaedic Research

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Mesenchymal stem cells (MSC) are increasingly replacing chondrocytes in tissue engineering based research for treatment of osteochondral defects. The aim of this work was to determine whether repair of critical-size chronic osteochondral defects in an ovine model using MSC-seeded triphasic constructs would show results comparable to osteochondral autografting (OATS). Triphasic implants were engineered using a beta-tricalcium phosphate osseous phase, an intermediate activated plasma phase, and a collagen I hydrogel chondral phase. Autologous MSCs were used to seed the implants, with chondrogenic predifferentiation of the cells used in the cartilage phase. Osteochondral defects of 4.0 mm diameter were created bilaterally in ovine knees (n = 10). Six weeks later, half of the lesions were treated with OATS and half with triphasic constructs. The knees were dissected at 6 or 12 months. With the chosen study design we were not able to demonstrate significant differences between the histological scores of both groups. Subcategory analysis of O'Driscoll scores showed superior cartilage bonding in the 6-month triphasic group compared to the autograft group. The 12-month autograft group showed superior cartilage matrix morphology compared to the 12-month triphasic group. Macroscopic and biomechanical analysis showed no significant differences at 12 months. Autologous MSC-seeded triphasic implants showed comparable repair quality to osteochondral autografts in terms of histology and biomechanical testing.

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Section: Journal Of Orthopaedic Research December 2010mentioning

confidence: 98%

A novel MSC‐seeded triphasic construct for the repair of osteochondral defects

Marquaß

Somerson

Hepp

et al. 2010

Journal Orthopaedic Research

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“…MSCs derived from non-cartilaginous knee joint tissues such as the infrapatellar fat pad (IFP) (Dragoo et al 2003;English et al 2007;Jurgens et al 2009;Buckley et al 2010a; Buckley et al 2010b) and synovium (Sakaguchi et al 2005;Yokoyama et al 2005; Mochizuki et al 2006;Pei et al 2008;Pei et al 2009;Sampat et al 2011) have been shown to possess significant chondrogenic potential. One of the key challenges in cartilage tissue engineering is ensuring sufficient functionality of the construct to be implanted which is capable of withstanding the challenging mechanical environment of the joint.…”

Section: Introductionmentioning

confidence: 99%

Expansion in the presence of FGF-2 enhances the functional development of cartilaginous tissues engineered using infrapatellar fat pad derived MSCs

Buckley

Kelly

2012

Journal of the Mechanical Behavior of Biomedical Materials

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A bstractMSCs from non-cartilaginous knee joint tissues such as the infrapatellar fat pad (IFP) and synovium possess significant chondrogenic potential and provide a readily available and clinically feasible source of chondroprogenitor cells. Fibroblast growth factor-2 (FGF-2) has been shown to be a potent mitotic stimulator during ex vivo expansion of MSCs, as well as regulating their subsequent differentiation potential.The objective of this study was to investigate the longer term effects of FGF-2 expansion on the functional development of cartilaginous tissues engineered using MSCs derived from the IFP. IFP MSCs were isolated and expanded to passage 2 in a standard media formulation with or without FGF-2 (5ng/ml) supplementation.Expanded cells were encapsulated in agarose hydrogels, maintained in chondrogenic media for 42 days and analysed to determine their mechanical properties and biochemical composition. Culture media, collected at each feed, was also analysed for biochemical constituents.MSCs expanded in the presence of FGF-2 proliferated more rapidly, with higher cell yields and lower population doubling times. FGF-2 expanded MSCs generated the most mechanically functional tissue. Matrix accumulation was dramatically higher after 21 days for FGF-2 expanded MSCs, but decreased between day 21 and 42. By day 42, FGF-2 expanded MSCs had still accumulated ~1.4 fold higher sGAG and ~1.7 fold higher collagen compared to control groups. The total amount of sGAG synthesised (retained in hydrogels and released into the media) was ~ 2.4 fold higher for FGF-2 expanded MSCs, with only ~25% of the total amount generated being retained within the constructs. Further studies are required to investigate whether IFP derived MSCs have a diminished capacity to synthesise other matrix components important in the aggregation, assembly and retention of proteoglycans. In conclusion, expanding MSCs in the presence of FGF-2 rapidly accelerates chondrogenesis in 3D agarose cultures resulting in superior mechanical functionality.

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“…After 6 months, the defects were covered by a hyaline-like tissue, well adhered to the surrounding healthy cartilage, presenting collagen II and glycosaminoglycans. However, contamination with macrophages was an issue in the in vitro assays [59].…”

Section: Sm-mscs' Therapeutic Applicationsmentioning

confidence: 99%

Synovia-Derived Mesenchymal Stem Cell Application in Musculoskeletal Injuries: A Review

Branquinho¹,

Caseiro²,

SantosPedrosa³

et al. 2018

Tissue Regeneration

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Musculoskeletal injuries impact millions of people globally and affect their health and well-being as well as of their companion and athletic animals. Soft-tissue injuries represent almost half of these and are associated with unorganized scar tissue formation and long time-depending healing processes. Cell-based therapeutic strategies have been developed in the past decades aiming at the treatment and reversion of such disorders. Stem cells are fairly appealing in the field, being a responsive undifferentiated population, with ability to self-renew and differentiate into different lineages. Mesenchymal stem cells (MSCs) can be obtained from several adult tissues, including the synovial membrane. Synovia-derived MSCs can be found in individuals of any age and are associated to intrinsic regenerative processes, through both paracrine and cell-to-cell interactions, thus, contributing to hosts' healing capacity. Studies have demonstrated the potential benefit of synovia-derived MSCs in these regenerative processes in both human and veterinary medicine. The purpose of this chapter is to review the literature regarding SM-MSC therapies applied to musculoskeletal disorders, in both human and veterinary medicine.

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Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs

Cited by 127 publications

References 43 publications

A novel MSC‐seeded triphasic construct for the repair of osteochondral defects

A novel MSC‐seeded triphasic construct for the repair of osteochondral defects

Expansion in the presence of FGF-2 enhances the functional development of cartilaginous tissues engineered using infrapatellar fat pad derived MSCs

Synovia-Derived Mesenchymal Stem Cell Application in Musculoskeletal Injuries: A Review

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