Repair of Injury in Freeze-Dried Salmonella anatum1

Ray, Bibek; Jezeski, J. J.; Busta, F. F.

doi:10.1128/aem.22.3.401-407.1971

Cited by 31 publications

(18 citation statements)

References 16 publications

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“…Repair in the presence of pyruvate also was inhibited by cyanide and DNP (data not presented). DNP inhibition of repair of injury has been reported by several workers (10, 13,16,21,26). Increases in the amount of phosphate or citrate repair in the presence of MgSO, could be due to Mg2+.…”

Section: Resultsmentioning

confidence: 74%

“…The ability of some of the freeze-injured cells to repair in the presence of phosphate or citrate indicates that the repair process, at least in these cells, was related to energy metabolism. Energy metabolism during repair of injury by cells that survived sublethal stresses has been reported (10,13,16,21,25). Citrate transport in many citrate-utilizing species in the Enterobacteriaceae depends upon a specific inducible permease system (9,26).…”

Section: Resultsmentioning

confidence: 99%

“…Carolina State University, Raleigh, N.C. 27607. tetraacetic acid treatment (10), N-hydroxyurethan treatment (16), X-ray radiation (14), and, possibly, air drying (28) required energy metabolism during repair. In many cases the energy synthesis possibly occurred in the form of adenosine triphosphate (ATP) synthesis by oxidative phosphorylation through the electron transport system (10,13,16,21,25).…”

Section: 'Present Address: Department Of Food Science Northmentioning

confidence: 99%

“…Microbial cell injury has been induced by various sublethal stresses (10,19,20,(22)(23)(24)(25)27). When the stress was removed 'and the cells were exposed to a suitable environment, the repair of damage in these cells was observed (10,12,15,16,21,25). The nature of the injury produced by exposing the microbial cells to different environmental stresses and cell repair mechanisms have been studied.…”

mentioning

confidence: 99%

“…Determination of cell injury and repair. A method described previously was used with the following slight modification (21). A 1-ml portion of the thawed sample was diluted in 9 ml of water.…”

mentioning

confidence: 99%

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Characterization of the Repair of Injury Induced by Freezing Salmonella anatum1

Ray

Janssen

Busta

1972

Applied Microbiology

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Fast freezing and slow thawing of Salmonella anatum cells suspended in water resulted in injury of more than 90% of the cells that survived the treatment. The injured cells failed to form colonies on the selective medium (xyloselysine-peptone-agar with 0.2% sodium deoxycholate) but did form colonies on a nonselective (xylose-lysine-peptone-agar) plating medium. In Tryptic soy plus 0.3% yeast extract broth or minimal broth, most of the injured cells repaired within 1 to 2 hr at 25 C. Tryptic soy plus yeast extract broth supported repair to a greater extent than minimal broth. Phosphate or citrate at concentrations found in minimal broth supported repair of some cells. MgSO4, when present with inorganic phosphate or citrate or both, increased the extent of repair. The repair process in the presence of phosphate was not prevented by actinomycin D, chloramphenicol, and D-cycloserine, but was prevented by cyanide and 2,4dinitrophenol (only at pH 6). This suggested that the repair process might involve energy metabolism in the form of adenosine triphosphate. The freeze-injured cells were highly sensitive to lysozyme, whereas unfrozen fresh cells were not. In the presence of phosphate or minimal broth this sensitivity was greatly reduced. This suggested that, at least in some of the cells, the injury involved the lipopolysaccharide of the cell wall and adenosine triphosphate synthesis-was required for repair.

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Section: Resultsmentioning

confidence: 74%