1981
DOI: 10.1007/bf00269658
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Repair of interstrand cross-links in DNA of Saccharomyces cerevisiae requires two systems for DNA repair: The RAD3 system and the RAD51 system

Abstract: We have studied the role of the excision-repair system and the recombination-repair system in the removal of cross-links and monoadducts caused by furocoumarins plus 360 nm radiation in yeast DNA by neutral and alkaline sucrose gradients and by a fluorometric procedure which detects cross-linked DNA molecules. We found that the excision-repair system, represented by the rad3 mutations, is required both for the removal of monoadducts, causing single-strand break formation, and for the removal of cross-links, ca… Show more

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Cited by 187 publications
(150 citation statements)
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“…The appearance of DSB as an intermediate in the ICL repair has been documented for S. cerevisiae (Jachymczyk et al, 1981;Magana-Schwencke et al, 1982;McHugh et al, 2000), and there is clear evidence that DSBs occur during ICL repair in mammalian cells as well (De Silva et al, 2000;Rothfuss and Grompe, 2004). hSNM1C/ ARTEMIS functions in the NHEJ pathway of DSB repair, where it complexes with DNA-PK CS and processes 5 0 and 3 0 overhangs (Ma et al, 2002).…”
Section: Discussionmentioning
confidence: 89%
“…The appearance of DSB as an intermediate in the ICL repair has been documented for S. cerevisiae (Jachymczyk et al, 1981;Magana-Schwencke et al, 1982;McHugh et al, 2000), and there is clear evidence that DSBs occur during ICL repair in mammalian cells as well (De Silva et al, 2000;Rothfuss and Grompe, 2004). hSNM1C/ ARTEMIS functions in the NHEJ pathway of DSB repair, where it complexes with DNA-PK CS and processes 5 0 and 3 0 overhangs (Ma et al, 2002).…”
Section: Discussionmentioning
confidence: 89%
“…In yeast cells the formation of psoralen-induced DSBs depends upon NER function (2,5,19,20). However, NER is dispensable for the generation of DSBs by nitrogen mustard cross-links in both yeast and mammalian cells (6,23).…”
Section: Discussionmentioning
confidence: 99%
“…Psoralen photoreaction of replicating yeast cells induces NER-dependent DNA breaks; monoadducts induce single-strand nicks, whereas interstrand cross-links induce DSBs (2,5,(17)(18)(19)(20). Rejoining of the cross-link-induced DSBs depends on the HR genes RAD51 and RAD52 (2,5,(21)(22)(23). These observations suggest the following two-step model for psoralen cross-link repair; 1) NER acts on cross-links to produce DSBs, and 2) the DSBs are repaired by HR.…”
mentioning
confidence: 99%
“…In the yeast Saccharomyces cerevisiae, excision of pyrimidine dimers or interstrand DNA crosslinks requires a large number of genes -RADI, RAD2, RAD3, RAD4, RAD1O, MMS19, RAD7, RAD14, RAD16, and RAD23 (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). A mutation in any of the first six of the ten genes listed results in highly defective incision of DNA containing pyrimidine dimers (13,14) or interstrand DNA crosslinks (8,11,12), while a mutation in RAD14, produces reduced incision proficiency compared with the Rad+ strain (9,13).…”
Section: Introductionmentioning
confidence: 99%
“…A mutation in any of the first six of the ten genes listed results in highly defective incision of DNA containing pyrimidine dimers (13,14) or interstrand DNA crosslinks (8,11,12), while a mutation in RAD14, produces reduced incision proficiency compared with the Rad+ strain (9,13). The RAD3 gene has been cloned and partially characterized (15,16).…”
Section: Introductionmentioning
confidence: 99%