Repair of Rat Calvarial Bone Defect by Using Exosomes of Umbilical Cord–Derived Mesenchymal Stromal Cells Embedded in Chitosan/Hydroxyapatite Scaffolds

Bahar, Dilek; Gönen, Zeynep Burçin; Gümüşderelı́oğlu, Menemşe; Önger, Mehmet Emin; Tokak, Elvan Konuk; Ozturk-Kup, Fatma; Ozkan, Buket Banu; Gokdemir, Nur Seda; Çetın, Mustafa

doi:10.11607/jomi.9515

Cited by 5 publications

(5 citation statements)

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“…Importantly, mechanistic studies revealed that exosomal miR-21 was the potential intercellular messenger that promoted angiogenesis by upregulating the NOTCH1/DLL4 pathway [ 146 ]. A human UC-MSC-derived exosome-loaded chitosan/hydroxyapatite (CS/HA) scaffold also regenerated significantly more bone than the scaffold-only group or the control group in a rat calvarial bone defect model [ 147 ].…”

Section: Umbilical Cord-derived Mesenchymal Stem Cells (Uc-mscs)mentioning

confidence: 99%

Stem Cells and Bone Tissue Engineering

Gao,

Ruzbarsky,

Layne

et al. 2024

Life

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Segmental bone defects that are caused by trauma, infection, tumor resection, or osteoporotic fractures present significant surgical treatment challenges. Host bone autograft is considered the gold standard for restoring function but comes with the cost of harvest site comorbidity. Allograft bone is a secondary option but has its own limitations in the incorporation with the host bone as well as its cost. Therefore, developing new bone tissue engineering strategies to treat bone defects is critically needed. In the past three decades, the use of stem cells that are delivered with different scaffolds or growth factors for bone tissue engineering has made tremendous progress. Many varieties of stem cells have been isolated from different tissues for use in bone tissue engineering. This review summarizes the progress in using different postnatal stem cells, including bone marrow mesenchymal stem cells, muscle-derived stem cells, adipose-derived stem cells, dental pulp stem cells/periodontal ligament stem cells, periosteum stem cells, umbilical cord-derived stem cells, peripheral blood stem cells, urine-derived stem cells, stem cells from apical papilla, and induced pluripotent stem cells, for bone tissue engineering and repair. This review also summarizes the progress using exosomes or extracellular vesicles that are delivered with various scaffolds for bone repair. The advantages and disadvantages of each type of stem cell are also discussed and explained in detail. It is hoped that in the future, these preclinical results will translate into new regenerative therapies for bone defect repair.

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Section: Umbilical Cord-derived Mesenchymal Stem Cells (Uc-mscs)mentioning

confidence: 99%

Stem Cells and Bone Tissue Engineering

Gao,

Ruzbarsky,

Layne

et al. 2024

Life

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show abstract

“…Importantly, mechanistic studies revealed that exosomal miR-21 was the potential intercellular messenger that promoted angiogenesis by upregulating the NOTCH1/DLL4 pathway [143]. Human UC-MSC-derived exosome-loaded chitosan/hydroxyapatite (CS/HA) scaffold also regenerated significantly more bone than the scaffold only group or the control group in a rat calvarial bone defect model [144].…”

Section: Uc-msc Derived Exosomes For Bone Tissue Engineeringmentioning

confidence: 99%

Stem Cells and Bone Tissue Engineering

Gao,

Ruzbarsky,

Layne

et al. 2024

Preprint

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Segmental bone defects caused by trauma, infection, tumor resection or osteoporotic fractures present significant surgical treatment challenges. Host bone autograft is considered the gold standard to restore function but comes with the cost of harvest site comorbidity. Allograft bone is a secondary option but has its own limitations in incorporation with host bone as well as cost. Therefore, developing new bone tissue engineering strategies to treat bone defects is critically needed. In the past three decades, the use of stem cells delivered with different scaffolds or growth factors for bone tissue engineering has made tremendous progress. Many varieties of stem cells have been isolated from different tissues for use in bone tissue engineering. This review summarizes the progress in using different postnatal stem cells including bone marrow mesenchymal stem cells, muscle-derived stem cells, adipose-derived stem cells, dental pulp stem cells/periodontal ligament stem cells, periosteum stem cells, umbilical cord-derived stem cells, peripheral blood stem cells and urine-derived stem cells for bone tissue engineering and repair. This review also summarizes the progress using exosomes or extracellular vesicles delivered with various scaffolds for bone repair. The advantages and disadvantages of each type of stem cell are also discussed and explained in detail. It is hopeful in the future that these preclinical results will translate into new therapies for bone defect repair.

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“…Aman et al prepared two types of cryogels, namely chitosan-gelatin-chondroitin sulfate (CGC) for articular cartilage and nanohydroxyapatite-gelatin (HG) for subchondral bone ( Bahar et al, 2022 ).A novel bilayer cryogel manufactured using a single process of two layers (CGC as top layer and HG as the bottom layer) to mimic osteochondral units. The CS/HA/Exo combination is a new therapy for bone defect repair to induce bone formation.…”

Section: Msc-exos In Combination With Biomaterials For Bone Defect Re...mentioning

confidence: 99%

MSC-Exos: Important active factor of bone regeneration

Ren

Lin

Liu

et al. 2023

Front. Bioeng. Biotechnol.

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Bone defect and repair is a common but difficult problem in restorative and reconstructive surgery. Bone tissue defects of different sizes caused by different reasons bring functional limitations and cosmetic deformities to patients. Mesenchymal stem cells (MSC), a major hotspot in the field of regeneration in recent years, have been widely used in various studies on bone tissue regeneration. Numerous studies have shown that the bone regenerative effects of MSC can be achieved through exosome-delivered messages. Although its osteogenic mechanism is still unclear, it is clear that MSC-Exos can directly or indirectly support the action of bone regeneration. It can act directly on various cells associated with osteogenesis, or by carrying substances that affect cellular activators or the local internal environment in target cells, or it can achieve activation of the osteogenic framework by binding to materials. Therefore, this review aims to summarize the types and content of effective contents of MSC-Exos in bone regeneration, as well as recent advances in the currently commonly used methods to enable the binding of MSC-Exos to the framework and to conclude that MSC-Exos is effective in promoting osteogenesis.

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Repair of Rat Calvarial Bone Defect by Using Exosomes of Umbilical Cord–Derived Mesenchymal Stromal Cells Embedded in Chitosan/Hydroxyapatite Scaffolds

Cited by 5 publications

References 0 publications

Stem Cells and Bone Tissue Engineering

Stem Cells and Bone Tissue Engineering

Stem Cells and Bone Tissue Engineering

MSC-Exos: Important active factor of bone regeneration

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