Repair of Rat Sciatic Nerve Gap by a Silk Fibroin-Based Scaffold Added with Bone Marrow Mesenchymal Stem Cells

Yang, Yumin; Yuan, Xiaohui; Ding, Fei; Yao, Dengbing; Gu, Yun; Liu, Jie; Gu, Xiaosong

doi:10.1089/ten.tea.2010.0633

Cited by 108 publications

(76 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This ability of MSC to potentiate axonal growth has been also described when the cells are transplanted distal to the MN soma. Thus, an increased rate of regeneration by MSC grafts was reported after sciatic nerve transection and repair with synthetic tubes [30] or after facial nerve transection [52]. In our in vitro model, we also observed that MSC enhance axonal regeneration, thus confirming the in vivo findings.…”

Section: Discussionsupporting

confidence: 88%

See 1 more Smart Citation

Neuroprotection and Axonal Regeneration After Lumbar Ventral Root Avulsion by Re-implantation and Mesenchymal Stem Cells Transplant Combined Therapy

et al. 2013

View full text Add to dashboard Cite

Ventral spinal root avulsion causes complete denervation of muscles in the limb and also progressive death of segmental motoneurons (MN) leading to permanent paralysis. The chances for functional recovery after ventral root avulsion are very poor owing to the loss of avulsed neurons and the long distance that surviving neurons have to re-grow axons from the spinal cord to the corresponding targets. Following unilateral avulsion of L4, L5 and L6 spinal roots in adult rats, we performed an intraspinal transplant of mesenchymal stem cells (MSC) and surgical re-implantation of the avulsed roots. Four weeks after avulsion the survival of MN in the MSC-treated animals was significantly higher than in vehicle-injected rats (45 % vs 28 %). Re-implantation of the avulsed roots in the injured spinal cord allowed the regeneration of motor axons. By combining root re-implantation and MSC transplant the number of surviving MN at 28 days post-injury was higher (60 %) than in re-implantation alone animals (46 %). Electromyographic tests showed evidence of functional re-innervation of anterior tibialis and gastrocnemius muscles by the regenerated motor axons only in rats with the combined treatment. These results indicate that MSC are helpful in enhancing neuronal survival and increased the regenerative growth of injured axons. Surgical re-implantation and MSC grafting combined had a synergic neuroprotective effect on MN and on axonal regeneration and muscle re-innervation after spinal root avulsion.

show abstract

Section: Discussionsupporting

confidence: 88%

“…Thus, after spinal cord injury [23][24][25][26] and brain stroke [27,28] focal injection of MSC improves functional recovery and reduces tissue damage and neuronal death. Furthermore, in experimental peripheral nerve injuries, MSC grafts have the capacity to enhance axonal regeneration [29,30].…”

Section: Introductionmentioning

confidence: 99%

Neuroprotection and Axonal Regeneration After Lumbar Ventral Root Avulsion by Re-implantation and Mesenchymal Stem Cells Transplant Combined Therapy

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Cellular prostheses composed of a resorbable guide seeded with autologous Schwann cells are an alternative for repairing long gaps in injured nerves, approaching the success of autografts [280,281]. However, conduits containing syngeneic Schwann cells did not improve the extent of axon regeneration with respect to acellular guides [280].…”

Section: Schwann Cells and Bone Marrow-derived Mesenchymal Stem Cellsmentioning

confidence: 99%

“…Filling a nerve gap with a 3-dimensional Matrigel promotes axon regeneration entirely across gaps [281,331]. This influence is increased if Schwann cells overexpressing high molecular weight FGF-2 is combined with the Matrigel [287].…”

Section: -Dimensional Scaffoldsmentioning

confidence: 99%

Promoting Axon Regeneration and Neurological Recovery Following Traumatic Peripheral Nerve Injuries

Kuffler¹

2015

Int J Neurorehabilitation Eng

View full text Add to dashboard Cite

Repairing Peripheral Nerves following Traumas Nerve crushWithin several days of crushing a peripheral nerve, the injured axons begin to regenerate through their distal pathway until they reach their original targets with which they restore neurological function. The larger the number of axons that regenerate through the distal nerve, the greater the extent of neurological recovery [1][2][3]. The number of axons that regenerate, and thus neurological recovery, is influenced by the physiological state of the distal denervated nerve pathway [4][5][6][7].Following a peripheral nerve crush, the injured axons begin to regenerate within 2 days of injury [8]. They may regenerate to their targets through their original pathway in association with and promoted by its Schwann cells, the neurotrophic factors they release, and the Schwann cell extracellular matrix [1,4,[9][10][11][12][13][14]. Thus, the distal nerve provides a simple means for promoting and directing the regenerating axons to their original targets [15][16][17][18]. However, when a nerve has a gap, the gap must be bridged with a material that is permissive to and promotes axon regeneration across the entire gap to reach the distal nerve stump, through which the axons must then be able to regenerate.The Schwann cells in the denervated distal nerve release neurotrophic factors that promote axon regeneration, but also release extracellular matrix components that promote and inhibit axon regeneration [19][20][21][22][23][24][25][26]. Thus, regeneration through the distal nerve is a balance of the influences of factors that both promote and inhibit regeneration. If the Schwann cells are present, such as after a simple nerve crush, virtually 100% of the axons regenerate and they innervate all the denervated synaptic sites. If the Schwann cells within the distal nerve pathway are killed, leaving only the extracellular matrix intact, the number of axons that regenerates to their targets decreases by 94%. This is because the factors required to trigger the regenerating axons to branch at extracellular matrix branch points are missing, axons do not branch, and therefore each axon reinnervates only a single denervated muscle fiber. Thus, Schwann cells along the distal nerve pathway and a AbstractFollowing a peripheral nerve crush, or when a peripheral the nerve is transected and the nerve stumps anastomosed, neurological recovery is generally excellent. This is because the axons merely have to regenerate through the distal portion of the nerve through the existing extracellular matrix of the denervated Schwann cells that direct and promote them to their distant denervated motor and sensory targets. However, when a length of a peripheral nerve is destroyed, and anastomosis is not possible, the standard surgical repair technique is to graft a length/s of autologous sensory nerve into the gap. Neurological recovery is generally good if the nerve gap is <2 cm in length, the repair is performed <4 months post trauma, and the patients are <25 years of age. Because the nerve ...

show abstract

“…In cases where the extent of the lesion precludes the simple joining of the stumps, an available and widely used repair technique is tubulization. This technique, cells include the mesenchymal cells of the bone marrow and of the adipose tissue, as well as the actual Schwann cells (6,7,(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33) (Table 1). These cells can be applied directly after density gradient separation (Ficoll-Paque ® ) or be cultivated and differentiated in vitro for subsequent application, as is the case of stem cell differentiation into Schwann cells.…”

Section: Introductionmentioning

confidence: 99%

Regeneração de nervos periféricos: terapia celular e fatores neurotróficos

Sebben¹,

Lichtenfels²,

Silva

2011

Rev. bras. ortop.

View full text Add to dashboard Cite

Peripheral nerve trauma results in functional loss in the innervated organ, and recovery without surgical intervention is rare. Many surgical techniques can be used for nerve repair. Among these, the tubulization technique can be highlighted: this allows regenerative factors to be introduced into the chamber. Cell therapy and tissue engineering have arisen as an alternative for stimulating and aiding peripheral nerve regeneration. Therefore, the aim of this review was to provide a survey and analysis on the results from experimental and clinical studies that used cell therapy and tissue engineering as tools for optimizing the regeneration process. The articles used came from the LILACS, Medline and SciELO scientific databases. Articles on the use of stem cells, Schwann cells, growth factors, collagen, laminin and platelet-rich plasma for peripheral nerve repair were summarized over the course Rev Bras Ortop. 2011;46(6):643-49 of the review. Based on these studies, it could be concluded that the use of stem cells derived from different sources presents promising results relating to nerve regeneration, because these cells have a capacity for neuronal differentiation, thus demonstrating effective functional results. The use of tubes containing bioactive elements with controlled release also optimizes the nerve repair, thus promoting greater myelination and axonal growth of peripheral nerves. Another promising treatment is the use of platelet-rich plasma, which not only releases growth factors that are important in nerve repair, but also serves as a carrier for exogenous factors, thereby stimulating the proliferation of specific cells for peripheral nerve repair.

show abstract

Repair of Rat Sciatic Nerve Gap by a Silk Fibroin-Based Scaffold Added with Bone Marrow Mesenchymal Stem Cells

Cited by 108 publications

References 62 publications

Neuroprotection and Axonal Regeneration After Lumbar Ventral Root Avulsion by Re-implantation and Mesenchymal Stem Cells Transplant Combined Therapy

Neuroprotection and Axonal Regeneration After Lumbar Ventral Root Avulsion by Re-implantation and Mesenchymal Stem Cells Transplant Combined Therapy

Promoting Axon Regeneration and Neurological Recovery Following Traumatic Peripheral Nerve Injuries

Regeneração de nervos periféricos: terapia celular e fatores neurotróficos

Contact Info

Product

Resources

About