Repair of the injury to respiratory epithelial cells characteristic of asthma is stimulated by Allomyrina dichotoma agglutinin specific serum glycoproteins

Abstract

These results indicate that following mechanical injury to the epithelium, serum glycoproteins, not only provide a protective barrier, but also are involved in the initiation of wound closure.

Fetuin A Stabilizes m-Calpain and Facilitates Plasma Membrane Repair

“…6B). Previous studies had localized fetuin A labeling to cells adjacent to scrape zones of wounded monolayer cultures (45). However, its co-localization with membrane repair proteins was not investigated.…”

Glycosylation and annexin II cell surface translocation mediate airway epithelial wound repair

“…Monolayers were washed with phosphate-buffered saline (PBS) followed by the addition of DMEM ϩ 10% FBS. After 18 h in culture, protein collection and lectin precipitation was carried out as previously described (40). Biotinylated lectin (20 g of CPA or VVA, EY Laboratories) was added to the supernatant and incubated overnight at 4°C.…”

“…In both studies, the same global inhibitor of N-glycosylation was used, but the specific cell surface glycoproteins that were altered by this inhibition were not identified. Further work by Adam et al (1) has shown that in culture, functional carbohydrate structures can be identified on the surface of cells by lectin histochemistry and wound closure inhibition (40).…”

IL-13, Asthma and Glycosylation in Airway Epithelial Repair

“…In this study the authors demonstrated that blocking glucose, mannose, and glucosamine sites on corneal epithelial cell surfaces and/ or the epithelial basement membrane reversibly slows or inhibits epithelial migration [78]. Using the same culture model of bronchial epithelial repair, Patchell et al has demonstrated that following mechanical wounding of intact monolayers, the lectins Allomyrina dichotoma (AlloA) and chick pea agglutinin (CPA) differentially stain human airway epithelial cells in damaged areas relative to the staining of intact epithelial monolayers [89]. While AlloA positive staining cells are those that appear to be migrating from areas distant to the wound and accumulating in the wound, CPA positive staining cells are restricted to the leading edge of the wound.…”