Repair of the Orbital Wall Fractures in Rabbit Animal Model Using Nanostructured Hydroxyapatite-Based Implant

Abstract

Cellular uptake and cytotoxicity of nanostructured hydroxyapatite (nanoHAp) are dependent on its physical parameters. Therefore, an understanding of both surface chemistry and morphology of nanoHAp is needed in order to be able to anticipate its in vivo behavior. The aim of this paper is to characterize an engineered nanoHAp in terms of physico-chemical properties, biocompatibility, and its capability to reconstitute the orbital wall fractures in rabbits. NanoHAp was synthesized using a high pressure hydrothermal method and characterized by physico-chemical, structural, morphological, and optical techniques. X-ray diffraction revealed HAp crystallites of 21 nm, while Scanning Electron Microscopy (SEM) images showed spherical shapes of HAp powder. Mean particle size of HAp measured by DLS technique was 146.3 nm. Biocompatibility was estimated by the effect of HAp powder on the adhesion and proliferation of mesenchymal stem cells (MSC) in culture. The results showed that cell proliferation on powder-coated slides was between 73.4% and 98.3% of control cells (cells grown in normal culture conditions). Computed tomography analysis of the preformed nanoHAp implanted in orbital wall fractures, performed at one and two months postoperative, demonstrated the integration of the implants in the bones. In conclusion, our engineered nanoHAp is stable, biocompatible, and may be safely considered for reconstruction of orbital wall fractures.

Ultrasonography used in the diagnosis of chronic rhinosinusitis: From experimental imaging to clinical practice

“…Correlating allergic rhinitis cases with nasal sinus ultrasound could be a future step in the management of this pathology (13). Thus, we hope that our data will help shift the use of sonography from experimental to clinical practice in ENT and other allied specialties, such as ophthalmology, plastic surgery, and trauma surgery (14,15).…”

Biocompatible Materials for Orbital Wall Reconstruction—An Overview

“…The advantages of resorbable sheeting are wide availability, good maneuverability in the wound, the possibility of intraoperative contouring, and smooth surface and edges. Their disadvantages include their cost, the fact that they are not radiopaque (postoperative implant cannot be viewed), degradation of the material can cause contour loss, sterile inflammation, serous orbit drainage is less efficient than in titanium meshes, and long-term stability and support [ 4 , 76 ].…”

“…In all polymer constructions, often without major changes to the design file of the device, the starting material composition can be modified to induce nano- and microtopography, variation in chemical composition, and even crystallography [ 83 ]. Hydroxyapatite, a calcium phosphate, was used in some studies with polymers [ 76 , 86 ]. This led to the formation of a polymer-based material with nano-sized and fully crystallized ceramic granules at its surface [ 87 , 88 ].…”

Ocular implants-methods of ocular reconstruction following radical surgical interventions

“…Regardless of the surgical technique, there is always a preoperative assessment, taking into account the history of ocular pathology (congenital or acquired ocular affection), objective and paraclinical examination, patient requirements (discomfort, aesthetic aspect), associated general disorders, explanation of pathology and surgical technique (pre- and post-operative psychological counseling), and, last but not least, written consent of the patient [ 14 , 15 ].…”