Repeated antigen painting and sublingual immunotherapy in mice convert sublingual dendritic cell subsets

Zhang, Chenyang; Ohno, Tomohiro; Kang, Siwen; Takai, Toshiro; Azuma, Miyuki

doi:10.1016/j.vaccine.2014.08.013

Cited by 17 publications

(6 citation statements)

References 33 publications

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“…Studies on the oral (buccal and sublingual) mucosal application of FITC in mice reported that the number of MHC class II + DCs in the submucosal layer increased, peaking 6 h after the FITC painting and decreased at 24 h. 9 , 26 Moreover, the number of FITC + CD11c + DCs in dLNs was lower in buccal mucosal sensitization than in ear skin sensitization. 9 In the present study, we reported that VM sensitization also showed similar results to oral mucosal sensitization.…”

Section: Discussionmentioning

confidence: 99%

Hapten sensitization to vaginal mucosa induces less recruitment of dendritic cells accompanying TGF‐β‐expressing CD206⁺ cells compared with skin

Nakayama

Nishijo

Miyazawa

et al. 2022

Immunity Inflam & Disease

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Introduction: Contact hypersensitivity (CHS), a type of delayed-type hypersensitivity, is induced by hapten exposure to the skin and mucosa. We previously reported that, in a murine model of CHS, the vaginal mucosa (VM) sensitization showed lower T-cell responses as compared with the abdominal skin sensitization. To investigate mechanisms of impaired CHS by the VM sensitization, we compared migration of hapten-captured dendritic cells (DCs) in the draining lymph nodes (dLNs) and recruitment of DCs at the sensitized local sites.Methods: Fluorescein isothiocyanate (FITC) or 2,4-dinitrofluorobenzene (DNFB) was used as hapten, and migration of FITC + DCs in the dLNs and local recruitment of MHC class II + and CD11c + cells were compared between abdominal skin and VM sensitization by flow cytometric analyses and immunohistochemistry. Expression of tumor growth factor (TGF)-β at mRNA and protein levels, and local recruitment of CD206 + cells were examined after VM sensitization.Results: VM sensitization showed less numbers of FITC + MHC class IIhigh CD11c + migratory DCs in the dLNs at 6 and 24 h, as compared with skin sensitization. Both skin and VM sensitization induced the recruitment of dermal/submucosal DCs at 6 h, but the number of submucosal DCs in the VM was significantly decreased at 24 h. VM showed persistently higher mRNA levels of TGF-β2/β3 expression than those of the skin before and after sensitization. In the VM sensitization, increment of CD206 + MHC class II + cells was observed especially at the deep lamina propria at 24 h. Most of CD206 + cells were also positive for the binding to Fc chimeric TGF-β receptor that interacts with all TGF-β isoforms, suggesting TGF-β expression. Conclusion:DC migration to dLNs and localization of DCs at the sensitized sites are limited in the VM sensitization. Our results suggest that the existence

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Section: Discussionmentioning

confidence: 99%

Hapten sensitization to vaginal mucosa induces less recruitment of dendritic cells accompanying TGF‐β‐expressing CD206⁺ cells compared with skin

Nakayama

Nishijo

Miyazawa

et al. 2022

Immunity Inflam & Disease

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“…3). However, it is possible that the circadian clock activity may affect other factors, such as the number and phenotypes of sublingual mucosa dendritic cells that are associated with tolerance induction by SLIT [16], thereby driving the daily variations of the SLIT efficacy.…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Sublingual Immunotherapy for Allergic Rhinitis May Vary with the Time of Day

Igarashi

Suzuki²,

Nakamura³

et al. 2016

Int Arch Allergy Immunol

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Background: Sublingual immunotherapy (SLIT) is a safe and effective treatment for allergic rhinitis (AR). However, many issues regarding SLIT remain to be resolved, including the optimal timing of administration. This study investigated the effect of time of day on SLIT efficacy with the goal of optimizing the therapeutic outcome. Methods: We performed prophylactic SLIT at different times of day (10 a.m. or 10 p.m.) in 2 mouse models of AR: an ovalbumin (OVA)-induced AR model and Cry j 1-induced AR model, and compared the effects. Results: In the OVA-induced AR model, mice sublingually receiving OVA at 10 a.m. exhibited a greater decrease in total and OVA-specific IgE levels than mice treated at 10 p.m. In addition, mice treated at 10 a.m. exhibited reductions in OVA-specific IL-4, IL-10, and IL-13 production by splenocytes relative to mice treated at 10 p.m. Furthermore, we observed a more efficient capture of sublingually administered OVA in submandibular lymph nodes at 10 a.m. than at 10 p.m. in mice. Similar results were observed in the Cry j 1-induced AR model using Japanese cedar pollen extract for SLIT. Conclusions: Given the allergen-specific antibody and T cell responses, we suggest that SLIT may be more effective in the resting phase than in the active phase (note that mice are nocturnal animals). Thus, we propose that a chronotherapeutic approach should be considered for SLIT to maximize its effectiveness.

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“…Due to limitations of currently available dosage forms (reviewed in Section ), the effect of sustained vaccine delivery in the oral mucosa on resulting immune responses is not well understood. While the majority of existing data on oral mucosal vaccination have delivered a bolus of vaccine, one study delivered low doses of antigen at narrow time intervals to achieve tolerance . With repeated dosing, DC morphology changed to a more rounded structure and had reduced capacity for migration to the draining lymph node.…”

Section: The Oral Mucosa Is a Promising And Underutilized Route Of Vamentioning

confidence: 99%

Microneedle‐Mediated Vaccine Delivery to the Oral Mucosa

Creighton

Woodrow

2018

Adv Healthcare Materials

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The oral mucosa is a minimally invasive and immunologically rich site that is underutilized for vaccination due to physiological and immunological barriers. To develop effective oral mucosal vaccines, key questions regarding vaccine residence time, uptake, adjuvant formulation, dose, and delivery location must be answered. However, currently available dosage forms are insufficient to address all these questions. An ideal oral mucosal vaccine delivery system would improve both residence time and epithelial permeation while enabling efficient delivery of physicochemically diverse vaccine formulations. Microneedles have demonstrated these capabilities for dermal vaccine delivery. Additionally, microneedles enable precise control over delivery properties like depth, uniformity, and dosing, making them an ideal tool to study oral mucosal vaccination. Select studies have demonstrated the feasibility of microneedle‐mediated oral mucosal vaccination, but they have only begun to explore the broad functionality of microneedles. This review describes the physiological and immunological challenges related to oral mucosal vaccine delivery and provides specific examples of how microneedles can be used to address these challenges. It summarizes and compares the few existing oral mucosal microneedle vaccine studies and offers a perspective for the future of the field.

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Repeated antigen painting and sublingual immunotherapy in mice convert sublingual dendritic cell subsets

Cited by 17 publications

References 33 publications

Hapten sensitization to vaginal mucosa induces less recruitment of dendritic cells accompanying TGF‐β‐expressing CD206⁺ cells compared with skin

Hapten sensitization to vaginal mucosa induces less recruitment of dendritic cells accompanying TGF‐β‐expressing CD206⁺ cells compared with skin

The Efficacy of Sublingual Immunotherapy for Allergic Rhinitis May Vary with the Time of Day

Microneedle‐Mediated Vaccine Delivery to the Oral Mucosa

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Repeated antigen painting and sublingual immunotherapy in mice convert sublingual dendritic cell subsets

Cited by 17 publications

References 33 publications

Hapten sensitization to vaginal mucosa induces less recruitment of dendritic cells accompanying TGF‐β‐expressing CD206+ cells compared with skin

Hapten sensitization to vaginal mucosa induces less recruitment of dendritic cells accompanying TGF‐β‐expressing CD206+ cells compared with skin

The Efficacy of Sublingual Immunotherapy for Allergic Rhinitis May Vary with the Time of Day

Microneedle‐Mediated Vaccine Delivery to the Oral Mucosa

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Product

Resources

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Hapten sensitization to vaginal mucosa induces less recruitment of dendritic cells accompanying TGF‐β‐expressing CD206⁺ cells compared with skin

Hapten sensitization to vaginal mucosa induces less recruitment of dendritic cells accompanying TGF‐β‐expressing CD206⁺ cells compared with skin