Repeated assessment of cardiovascular and respiratory functions using combined telemetry and whole-body plethysmography in the rat

Delaunois, Annie; Dedoncker, Paul; Hanon, Étienne; Guyaux, Michel

doi:10.1016/j.vascn.2009.07.003

Cited by 33 publications

(11 citation statements)

References 17 publications

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“…However, a fundamental cause of the large variability in resting HR observed in the present study has not been determined. The range of the resting HR obtained in the present study was similar to that obtained in conscious unstrained rats (Delaunois et al, 2009; Albrecht et al, 2014; Sharp et al, 2014). …”

Section: Discussionsupporting

confidence: 90%

Heart Rate Changes in Response to Mechanical Pressure Stimulation of Skeletal Muscles Are Mediated by Cardiac Sympathetic Nerve Activity

Watanabe

Hotta

2017

Front. Neurosci.

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Stimulation of mechanoreceptors in skeletal muscles such as contraction and stretch elicits reflexive autonomic nervous system changes which impact cardiovascular control. There are pressure-sensitive mechanoreceptors in skeletal muscles. Mechanical pressure stimulation of skeletal muscles can induce reflex changes in heart rate (HR) and blood pressure, although the neural mechanisms underlying this effect are unclear. We examined the contribution of cardiac autonomic nerves to HR responses induced by mechanical pressure stimulation (30 s, ~10 N/cm2) of calf muscles in isoflurane-anesthetized rats. Animals were artificially ventilated and kept warm using a heating pad and lamp, and respiration and core body temperature were maintained within physiological ranges. Mechanical stimulation was applied using a stimulation probe 6 mm in diameter with a flat surface. Cardiac sympathetic and vagus nerves were blocked to test the contribution of the autonomic nerves. For sympathetic nerve block, bilateral stellate ganglia, and cervical sympathetic nerves were surgically sectioned, and for vagus nerve block, the nerve was bilaterally severed. In addition, mass discharges of cardiac sympathetic efferent nerve were electrophysiologically recorded. Mechanical stimulation increased or decreased HR in autonomic nerve-intact rats (range: −56 to +10 bpm), and the responses were negatively correlated with pre-stimulus HR (r = −0.65, p = 0.001). Stimulation-induced HR responses were markedly attenuated by blocking the cardiac sympathetic nerve (range: −9 to +3 bpm, p < 0.0001) but not the vagus nerve (range: −75 to +30 bpm, p = 0.17). In the experiments with cardiac sympathetic efferent nerve activity recordings, mechanical stimulation increased, or decreased the frequency of sympathetic nerve activity in parallel with HR (r = 0.77, p = 0.0004). Furthermore, the changes in sympathetic nerve activity were negatively correlated with its tonic level (r = −0.62, p = 0.0066). These results suggest that cardiac sympathetic nerve activity regulates HR responses to muscle mechanical pressure stimulation and the direction of HR responses depends on the tonic level of the nerve activity, i.e., bradycardia occurs when the tonic activity is high and tachycardia occurs when the activity is low.

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Section: Discussionsupporting

confidence: 90%

Heart Rate Changes in Response to Mechanical Pressure Stimulation of Skeletal Muscles Are Mediated by Cardiac Sympathetic Nerve Activity

Watanabe

Hotta

2017

Front. Neurosci.

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“…Administration of SFSE-G significantly inhibited alteration in weight body loss and lung index which might be due to inhibition of inflammatory influx in the lung. Inhalation of the toxicant resulted in the alteration of the respiratory patterns which can be assessed by measuring the pulmonary function and determination of pulmonary function tests (pfts) using whole body plethysmography is useful non-invasive tool for the diagnosis of the nature and severity of lung injury irrespective of its mechanisms of injury [23]. The significant increase in the airway obstruction i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Recorded variables included peak inspiratory flow (PIF), Peak expiratory flow (PEF), tidal volume (TV), expired volume (EV), frequency of breathing (f), enhanced pause (P enh ) value [23].…”

Section: Lung Function Measurementsmentioning

confidence: 99%

Effect of glycosides based standardized fenugreek seed extract in bleomycin-induced pulmonary fibrosis in rats: Decisive role of Bax, Nrf2, NF-κB, Muc5ac, TNF-α and IL-1β

Kandhare

Bodhankar

Mohan

et al. 2015

Chemico-Biological Interactions

134

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“…At the end of the 90 min period the animals were removed from the chambers for oral (by gavage) dosing of theophylline (3, 10, 30 mg/kg), clonidine (0.03, 0.1, 0.3 mg/kg), baclofen (1, 3, 10 mg/kg) or vehicle (water for injection) and immediately returned to the chambers. Measurements were continued for approximately 4 h. Dose levels were selected based on information derived from the literature (Delaunois, Dedoncker, Hanon, & Guyaux, 2009;Himmel, 2008). It is noteworthy that animals in the AZUSA facility were habituated to the plethysmography chamber for up to 4 h (progressively over several days) prior to the day of dosing.…”

Section: Rat Whole Body Plethysmography For Respiratory Assessmentmentioning

confidence: 99%

A multi-site comparison of in vivo safety pharmacology studies conducted to support ICH S7A & B regulatory submissions

Ewart

Milne

Adkins

et al. 2013

Journal of Pharmacological and Toxicological Methods

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Repeated assessment of cardiovascular and respiratory functions using combined telemetry and whole-body plethysmography in the rat

Cited by 33 publications

References 17 publications

Heart Rate Changes in Response to Mechanical Pressure Stimulation of Skeletal Muscles Are Mediated by Cardiac Sympathetic Nerve Activity

Heart Rate Changes in Response to Mechanical Pressure Stimulation of Skeletal Muscles Are Mediated by Cardiac Sympathetic Nerve Activity

Effect of glycosides based standardized fenugreek seed extract in bleomycin-induced pulmonary fibrosis in rats: Decisive role of Bax, Nrf2, NF-κB, Muc5ac, TNF-α and IL-1β

A multi-site comparison of in vivo safety pharmacology studies conducted to support ICH S7A & B regulatory submissions

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