Repeated assessment of orthotopic glioma pO2 by multi-site EPR oximetry: A technique with the potential to guide therapeutic optimization by repeated measurements of oxygen

Khan, Nadeem; Mupparaju, Sriram P.; Hou, Huagang; Williams, Benjamin B.; Swartz, Harold M.

doi:10.1016/j.jneumeth.2011.10.026

Cited by 23 publications

(18 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We furthermore showed a substantial difference between StO 2 estimates in the 9 L glioma model where mqBOLD indicates normal or higher StO 2 in the tumor, while MRvF indicates lower oxygenation. Previous studies using Electron Paramagnetic Resonance (EPR) have reported normal tissue oxygenation in the 9 L model2728. However, other studies using oxygen microelectrodes have reported very low pO 2 measurements (hypoxic) in the 9 L tumors29.…”

Section: Discussionmentioning

confidence: 99%

MR Vascular Fingerprinting in Stroke and Brain Tumors Models

Lemasson

Pannetier

Coquery

et al. 2016

Sci Rep

View full text Add to dashboard Cite

In this study, we evaluated an MRI fingerprinting approach (MRvF) designed to provide high-resolution parametric maps of the microvascular architecture (i.e., blood volume fraction, vessel diameter) and function (blood oxygenation) simultaneously. The method was tested in rats (n = 115), divided in 3 models: brain tumors (9 L, C6, F98), permanent stroke, and a control group of healthy animals. We showed that fingerprinting can robustly distinguish between healthy and pathological brain tissues with different behaviors in tumor and stroke models. In particular, fingerprinting revealed that C6 and F98 glioma models have similar signatures while 9 L present a distinct evolution. We also showed that it is possible to improve the results of MRvF and obtain supplemental information by changing the numerical representation of the vascular network. Finally, good agreement was found between MRvF and conventional MR approaches in healthy tissues and in the C6, F98, and permanent stroke models. For the 9 L glioma model, fingerprinting showed blood oxygenation measurements that contradict results obtained with a quantitative BOLD approach. In conclusion, MR vascular fingerprinting seems to be an efficient technique to study microvascular properties in vivo. Multiple technical improvements are feasible and might improve diagnosis and management of brain diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

MR Vascular Fingerprinting in Stroke and Brain Tumors Models

Lemasson

Pannetier

Coquery

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…It has therefore proven difficult thus far to define the optimal fractionation scheme. This change in oxygenation could potentially be monitored by measuring the partial pressure of oxygen in the glioma and adjusting the duration of the on/off sequences [24].…”

Section: Discussionmentioning

confidence: 99%

Interstitial 5-ALA photodynamic therapy and glioblastoma: Preclinical model development and preliminary results

Tétard

Vermandel

Leroy

et al. 2016

Photodiagnosis and Photodynamic Therapy

View full text Add to dashboard Cite

We have developed a reproducible and reliable rodent model for interstitial 5-ALA PDT. We found that the effects of 5-ALA-PDT are dependent on light delivery conditions. Although the low-fluence-rate treatment was better tolerated, 5-ALA-PDT induced more necrosis using fractionated delivery at a high fluence rate. These results require confirmation with further studies involving larger populations and additional fractionation schemes.

show abstract

“…The method requires an injection of an exogenous probe bearing an unpaired electron that is selective in its interaction with oxygen (97). Recently, implantable and metabolically inert paramagnetic lithium phthalocyanine crystals have been utilized as oxygen-sensitive electron paramagnetic resonance (EPR) probes to monitor changes in tissue hypoxia (140). The width of the spectral band corresponding to the probe signal correlates with oxygen concentration.…”

Section: The Clinical Importance Of Assessing Tumor Hypoxiamentioning

confidence: 99%

The Clinical Importance of Assessing Tumor Hypoxia: Relationship of Tumor Hypoxia to Prognosis and Therapeutic Opportunities

Walsh

Lebedev

Aten³

et al. 2014

Antioxidants & Redox Signaling

358

317

View full text Add to dashboard Cite

Tumor hypoxia is a well-established biological phenomenon that affects the curability of solid tumors, regardless of treatment modality. Especially for head and neck cancer patients, tumor hypoxia is linked to poor patient outcomes. Given the biological problems associated with tumor hypoxia, the goal for clinicians has been to identify moderately to severely hypoxic tumors for differential treatment strategies. The ''gold standard'' for detecting and characterizing of tumor hypoxia are the invasive polarographic electrodes. Several less invasive hypoxia assessment techniques have also shown promise for hypoxia assessment. The widespread incorporation of hypoxia information in clinical tumor assessment is severely impeded by several factors, including regulatory hurdles and unclear correlation with potential treatment decisions. There is now an acute need for approved diagnostic technologies for determining the hypoxia status of cancer lesions, as it would enable clinical development of personalized, hypoxia-based therapies, which will ultimately improve outcomes. A number of different techniques for assessing tumor hypoxia have evolved to replace polarographic pO 2 measurements for assessing tumor hypoxia. Several of these modalities, either individually or in combination with other imaging techniques, provide functional and physiological information of tumor hypoxia that can significantly improve the course of treatment. The assessment of tumor hypoxia will be valuable to radiation oncologists, surgeons, and biotechnology and pharmaceutical companies who are engaged in developing hypoxia-based therapies or treatment strategies. Antioxid. Redox Signal. 21, 1516-1554.

show abstract

Repeated assessment of orthotopic glioma pO2 by multi-site EPR oximetry: A technique with the potential to guide therapeutic optimization by repeated measurements of oxygen

Cited by 23 publications

References 34 publications

MR Vascular Fingerprinting in Stroke and Brain Tumors Models

MR Vascular Fingerprinting in Stroke and Brain Tumors Models

Interstitial 5-ALA photodynamic therapy and glioblastoma: Preclinical model development and preliminary results

The Clinical Importance of Assessing Tumor Hypoxia: Relationship of Tumor Hypoxia to Prognosis and Therapeutic Opportunities

Contact Info

Product

Resources

About