Repeated batch and continuous degradation of chlorpyrifos by<i>Pseudomonas putida</i>

Pradeep, Vijayalakshmi; Subbaiah, Usha Malavalli

doi:10.1080/03601234.2015.1000180

Cited by 14 publications

(6 citation statements)

References 40 publications

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“…Pseudomonads can degrade a variety of pesticides, such as the organophosphorus insecticides methamidophos, chlorpyrifos, and methyl parathion; − the carbamate insecticides carbaryl, methomyl, and oxamyl; − and the neonicotinoid insecticides thiamethoxam and imidacloprid. , However, until now, no Pseudomonas strain has been reported to have pymetrozine-degrading abilities. In this study, strain BYT-1, with the capability of degrading pymetrozine, was isolated for the first time.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of the Pymetrozine-Degrading Strain Pseudomonas sp. BYT-1

Sun

Zhang

et al. 2019

J. Agric. Food Chem.

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In this study, we isolated and characterized the bacterial strain Pseudomonas sp. BYT-1, which is capable of degrading pymetrozine and using it as the sole carbon source for growth. Strain BYT-1 could degrade 2.30 mM pymetrozine within 20 h under the optimal conditions of 30 °C and pH 7.0. Investigation of the degradation pathway showed that pymetrozine was oxidatively hydrolyzed to 4-amino-6-methyl-4,5-dihydro-2H-[1,2,4]triazin-3-one (AMDT) and nicotinic acid (NA). The former accumulates as the end product in the culture, whereas the latter was hydroxylated to 6-hydroxynicotinic acid (6HNA) and subjected to further degradation. The transformation of pymetrozine to AMDT and NA by the cell-free extracts of strain BYT-1 also supported that the oxidative hydrolysis of the CN double bond in pymetrozine was the initial degradation step. This is the first report on a pure bacterial culture with the ability to degrade pymetrozine. These findings enhance our understanding of the microbial degradation mechanism of pymetrozine.

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Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of the Pymetrozine-Degrading Strain Pseudomonas sp. BYT-1

Sun

Zhang

et al. 2019

J. Agric. Food Chem.

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“…Pseudomonas putida was reported to metabolize Chlorpyrifos after the strain immobilized with calcium alginate, the products of degradation detected by LC-MS was found to be TCP and Chlorpyrifos oxon 20 . TCP was accumulated during the degradation process of 100 mg L -1 of Chlorpyrifos by Sphingomonas sp., which slowed down the degradation process 21 .…”

Section: Biodegradation Ability Of Staphylococcus Sp Es-2mentioning

confidence: 99%

Bio-Mineralization of Organophosphorous Insecticide-Chlorpyrifos and its Hydrolyzed Product 3,5,6-Trichloro-2-Pyridinol by Staphylococcus Sp. ES-2

Supreeth

Raju

2016

Curr. World Environ

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Application of Chlorpyrifos on agricultural fields to protect crops against pests results in accumulation of it in soil and other environmental samples. The insecticide transform into 3,5,6-Trichloro-2-Pyridinol (TCP) through hydrolysis in soil, which has got antimicrobial property and hence resists its degradation in natural condition. In the current findings, a bacterial isolate capable of mineralizing Chlorpyrifos without accumulation of TCP was isolated from agricultural soil by enrichment method. Based on Morphological, Biochemical Characterization and with Bergey’s Manual comparision, the isolate was identified as Staphylococcus sp. The isolate was found to metabolize chlorpyrifos completely in Mineral salt medium with chlorpyrifos as the sole carbon source. No metabolites of chlorpyrifos were detected in Liquid Chromatography-Mass Spectroscopy (LC-MS) analysis after 7 days of incubation. The novelty of the outcome of the experiment relies on Staphylococcus sp.ES-2 in complete mineralization of chlorpyrifos which can be used as a potential bioaugmenting agent in the chlorpyrifos contaminated sites.

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“…Microbial cell immobilization was initiated to deal with these problems. The immobilization techniques were successfully applied for pesticide biodegradations (Pradeep & Subbaiah, 2015;Juliana M. Saez, Aparicio, Amoroso, & Benimeli, 2015;Yáñez-Ocampo, Sánchez-Salinas, & Ortiz-Hernández, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…The result showed that the removal of PCB by the alginate-immobilized cells was greater than that by free cells. In addition, Pradeep and Subbaiah (2015) examined the repeating use of the immobilized P. putida for chlorpyrifos degradation. The result showed that the immobilized cell could degrade 70% of chlorpyrifos and was able to reuse for 50 times with less cell leakage (112 x 10 3 CFU/mL) (Pradeep & Subbaiah, 2015).…”

Section: Bioremediationmentioning

confidence: 99%

“…In addition, Pradeep and Subbaiah (2015) examined the repeating use of the immobilized P. putida for chlorpyrifos degradation. The result showed that the immobilized cell could degrade 70% of chlorpyrifos and was able to reuse for 50 times with less cell leakage (112 x 10 3 CFU/mL) (Pradeep & Subbaiah, 2015). Table 5 presents examples of immobilization technique is bioremediation application.…”

Section: Bioremediationmentioning

confidence: 99%

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Profenofos removal by pseudomonas plecoglossicida PF1 and acinetobacter baylyi GFJ2 in free and immobilized cell forms and their motility

Ploychankul

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Profenofos, a widely used pesticide, has been reported contamination in environment. This study investigated profenofos removal using the immobilized bacterial cells. Cell movement ability related to biodegradation performance was emphasized. Two profenofos-degrading strains, Pseudomonas plecoglossicida PF1 (PF1) and Acinetobacter baylyi GFJ2 (GFJ2) which were motile and non-motile bacteria, respectively were applied. The study divided into 2 parts. For the first part, characterization of profenofos biodegradation by PF1 and GFJ2 influencing by environmental conditions (effects of pH, temperature, and profenofos concentration) was performed. For the second part, the applications of the immobilized cells in batch (effects of the immobilized cell sizes and inorganic salts) and column (effect of profenofos concentrations) tests were examined to accomplish on the gap knowledge between the removal efficiency and behavior of the microorganisms after being immobilized in the matrices comparing to the free cell. The well-known cell entrapment matrix, calcium alginate (CA), was selected. The result showed that both PF1 and GFJ2 were the potential profenofos-degrading microorganisms with a high profenofos removal percentage (60-90%). Optimal conditions for profenofos biodegradation were at pHs of 5.30-7.87, temperatures of 20-40 °C, and profenofos concentrations of 10-200 mg/L. For the batch experiment, the bead size obviously affected the profenofos removal performance. The suitable bead size in this study was 4 mm-diameter. PF1 was positive in motility assays. PF1 got attraction by profenofos resulting in high removal efficiency. For the column experiment, the immobilization technique well retained both motile and non-motile cells. It was also found natural cell colonization on sand in the free cell columns led to similar profenofos removal performance by the free and immobilized cells (60-90%). For the long term, motile bacterium (PF1) either in free or immobilized cell forms performed better than GFJ2.

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Repeated batch and continuous degradation of chlorpyrifos byPseudomonas putida

Cited by 14 publications

References 40 publications

Isolation and Characterization of the Pymetrozine-Degrading Strain Pseudomonas sp. BYT-1

Isolation and Characterization of the Pymetrozine-Degrading Strain Pseudomonas sp. BYT-1

Bio-Mineralization of Organophosphorous Insecticide-Chlorpyrifos and its Hydrolyzed Product 3,5,6-Trichloro-2-Pyridinol by Staphylococcus Sp. ES-2

Profenofos removal by pseudomonas plecoglossicida PF1 and acinetobacter baylyi GFJ2 in free and immobilized cell forms and their motility

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