Repeated Batch Biotransformation from Isoeugenol to Vanillin by Immobilized <i>Bacillus fusiformis</i> Cells in CSTR Reactor

Abstract

Bacillus fusiformis CGMCC1347, screened from soil, was immobilized successfully to biotransform isoeugenol to vanillin. The optimal immobilization conditions were 2.5% sodium alginate, 10% cell density and Ca2+ 0.1 mol l-1. The optimal reaction conditions in a 2 L CSTR reactor with immobilized Bacillus fusiformis CGMCC1347 cells were isoeugenol 600 mL, immobilized cells 400 g, pH 3.5 dH2O 400 mL, 37 oC, 200 r min-1 shaking speed and 0.8 vvm air capacity. The average vanillin concentration reached 39.26 g l-1 after 6 times reuse of immobilized cells in 2 L CSTR.

Strategies for improving the production of bio-based vanillin

“…ISPC2 Bioreactor 1.15 g/L 12.4% (molar yield) / [ 19 ] Psychrobacter sp. CSW4 Bioreactor 1.28 g/L / / [ 61 ] Candida galli PG06 Bioreactor 1.12 g/L 25.7% (molar yield) / [ 20 ] Trichosporon asahii MP24 Bioreactor 2.40 g/L 52.5% (molar yield) / [ 21 ] Bacillus fusiformis CGMCCl347 Bioreactor 3.25 g/L / / [ 44 ] Bacillus fusiformis CGMCC1347 with utilization of HD-8 resin Bioreactor 8.10 g/L 0.29 g/g Isoeugenol / [ 62 ] Bacillus fusiformis CGMCC1347 with utilization of HD-8resin after 6 times reuse of immobilized cells Bioreactor 39.26 g/L / / [ 63 ] E. coli BL21(DE3) with pET-21a- iem Bioreactor / 0.76 g/g Isoeugenol 115 g/L/d [ 46 …”