Repeated-batch fermentations of xylose and glucose–xylose mixtures using a respiration-deficient Saccharomyces cerevisiae engineered for xylose metabolism

Kim, Soo Rin; Lee, Ki Sung; Choi, Jinho; Ha, Suk Jin; Kweon, Dae Hyuk; Seo, Jin Ho; Jin, Yong Su

doi:10.1016/j.jbiotec.2010.09.962

Cited by 20 publications

(11 citation statements)

References 32 publications

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“…Repeated batch is one of the techniques for the cell recycle in the bioprocess. This provides the advantages in terms of reducing in the time and cost associated with inoculum preparation [30,39]. Repeated batch was performed by collecting the adsorbed cells on DEAE-cellulose from the previous batch and reinoculated into a new rice bran hydrolysate.…”

Section: Resultsmentioning

confidence: 99%

Fermentation of rice bran hydrolysate to ethanol using Zymomonas mobilis biofilm immobilization on DEAE-cellulose

Todhanakasem

Narkmit

Areerat

et al. 2015

Electronic Journal of Biotechnology

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Background:The major challenges associated with the fermentation of lignocellulosic hydrolysates are the reduction in the operating cost and minimizing the complexity of the process. Zymomonas mobilis biofilm has been emerged to resolve these complexities. Biofilm has been reported to tolerate to the toxic inhibitors and easily manipulated toward the cell recycle through the cell immobilization. Results: Z. mobilis ZM4 and TISTR 551 were able to develop biofilms on DEAE cellulose under the differences in the morphologies. Z. mobilis ZM4 developed homogeneous biofilm that brought DEAE fiber to be crosslinking, while Z. mobilis TISTR 551 developed heterogeneous biofilm in which crosslinking was not observed. Ethanol production under batch and repeated batch fermentation of rice bran hydrolysate containing toxic inhibitors were compared between these two biofilms. TISTR 551 biofilm produced the maximum yield (Y P/S ) of 0.43 ± 0.09 g ethanol/g glucose (83.89% theoretical yield). However the repeated batch could not be proceeded due to the bacterial detachment. Z. mobilis ZM4 biofilm produced the maximum yield (Y P/S ) of 0.177 ± 0.05 g ethanol/g glucose (34.74% theoretical yield) in the batch culture and the biofilm remained intact to proceed along the repeated batch. The highest ethanol yield (Y P/S ) in the repeated batch of Z. mobilis ZM4 was 0.354 ± 0.07 g ethanol/g glucose (69.51% theoretical yield). Conclusions: Homogeneous biofilm structure of Z. mobilis provided more recycle beneficial over the heterogeneous biofilm structure for the ethanol production from lignocellulosic hydrolysate.

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Section: Resultsmentioning

confidence: 99%

Fermentation of rice bran hydrolysate to ethanol using Zymomonas mobilis biofilm immobilization on DEAE-cellulose

Todhanakasem

Narkmit

Areerat

et al. 2015

Electronic Journal of Biotechnology

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“…Many reports have suggested that aeration and oxygen supply can greatly affect xylose consumption (Jeffries, ; Jeffries & Jin, ; Jin et al, ; Kastner, Jones, & Roberts, ; Kim et al, ). Fundamental differences in oxygen requirements between growth in xylose and glucose have been observed in fungi and oxygen starvation studies showed induction of cell death on xylose but not glucose in the yeast strain Candida shehatae (Kastner et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Many reports have suggested that aeration and oxygen supply can greatly affect xylose consumption (Jeffries, 2006;Jeffries & Jin, 2004;Jin et al, 2004a;Kastner, Jones, & Roberts, 1999;Kim et al, 2010).…”

Section: Characterization Of the Improved Mutantsmentioning

confidence: 99%

RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization

HamediRad¹,

Lian

et al. 2018

Biotech & Bioengineering

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Xylose is a major component of lignocellulosic biomass, one of the most abundant feedstocks for biofuel production. Therefore, efficient and rapid conversion of xylose to ethanol is crucial in the viability of lignocellulosic biofuel plants. In this study, RNAi Assisted Genome Evolution (RAGE) was used to improve the xylose utilization rate in SR8, one of the most efficient publicly available xylose utilizing Saccharomyces cerevisiae strains. To identify gene targets for further improvement, we created a genome-scale library consisting of both genetic over-expression and down-regulation mutations in SR8. Followed by screening in media containing xylose as the sole carbon source, yeast mutants with 29% faster xylose utilization, and 45% higher ethanol productivity were obtained relative to the parent strain. Two known and two new effector genes were identified in these mutant strains. Notably, down-regulation of CDC11, an essential gene, resulted in faster xylose utilization, and this gene target cannot be identified in genetic knock-out screens.

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“…Repeated-batch fermentation is a promising method to increase ethanol production with a high cell density as well as to reduce manufacturing costs (Kim et al, 2010b;Yamakawa et al, 2010). Furthermore, flocculation characteristics might be required for efficient recycling of cells without centrifugation (Silva et al, 2010;Zhao and Bai, 2009).…”

Section: Discussionmentioning

confidence: 99%

Efficient Production of Ethanol from Saccharified Crops Mixed with Cheese Whey by the Flex Yeast Kluyveromyces marxianus KD-15

Nakamura

Shinomiya²,

Orikasa

et al. 2012

FSTR

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Ethanol production from a mixture of wheat flour or potato tubers and cheese whey was examined using the flex yeast Kluyveromyces marxianus KD-15, a 2-deoxyglucose-resistant mutant of strain NBRC 1963 that can produce ethanol from sugar beet thick juice diluted with whey. Strain KD-15 simultaneously converted glucose and lactose to ethanol within 48 h, in media containing 10.0% to 15.0% (w/v) total sugars from saccharified filtrate and whey. For efficient production of ethanol from 15.0% (w/v) total sugars, KD-15 cells were collected after fermentation and inoculated into fresh media. Batch fermentation was successfully repeated at least ten times in medium composed of saccharified potato tubers mixed with whey. Yeast cells began to convert all the sugars to ethanol within 24 h, accompanied by cell propagation after the third batch.

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Repeated-batch fermentations of xylose and glucose–xylose mixtures using a respiration-deficient Saccharomyces cerevisiae engineered for xylose metabolism

Cited by 20 publications

References 32 publications

Fermentation of rice bran hydrolysate to ethanol using Zymomonas mobilis biofilm immobilization on DEAE-cellulose

Fermentation of rice bran hydrolysate to ethanol using Zymomonas mobilis biofilm immobilization on DEAE-cellulose

RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization

Efficient Production of Ethanol from Saccharified Crops Mixed with Cheese Whey by the Flex Yeast Kluyveromyces marxianus KD-15

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