Repeated batch production of agar-oligosaccharides from agarose by an amberlite IRA-900 immobilized agarase system

Koti, Basawaraj A.; Shinde, Manohar; Lalitha, Junna

doi:10.1007/s12257-012-0237-5

Cited by 15 publications

(14 citation statements)

References 24 publications

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“…The advantages of immobilized enzymes include the ease of enzyme recovery from the reaction mixture, ease of controlling the de-polymerization product, rapid termination of the enzyme reaction, and feasibility for application in various engineering designs [ 48 ]. For instance, an immobilized agarase, obtained from Pseudomonas aeruginosa AG LSL-11, appeared to be more stable and had higher activity compared to the free enzyme [ 49 ]. Similarly, Xiao et al reported a ҡ-carrageenase that was immobilized onto magnetic iron oxide nanoparticles.…”

Section: Production Of Oligosaccharide From Red Seaweedmentioning

confidence: 99%

“…The stationary phase is a material of silica or polymer particles modified with different types of polar functional groups, such as amino, amide, diol, cyano etc., while the mobile phase is in combination with the organic mobile phase (usually acetonitrile) and elution is performed by increasing the water concentration [ 66 , 67 ]. For example, the AOS, produced from agar hydrolyzed by immobilized agarase, can be separated by an amino column using 0.1% formic acid in acetonitrile as the mobile phase [ 49 ].…”

Section: Chromatographic Separation Of Oligosaccharidesmentioning

confidence: 99%

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Oligosaccharides Derived from Red Seaweed: Production, Properties, and Potential Health and Cosmetic Applications

et al. 2018

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Because of their potential use as functional ingredients in human nutrition, oligosaccharides derived from natural sources are receiving paramount consideration. Red seaweed, a proven rich source of agar and carrageenan, is one of the most abundantly present sources of such oligosaccharides. Agaro-oligosaccharides (AOS) and carrageenan-oligosaccharides (COS) are produced from agar and carrageenan, respectively, through chemical and enzymatic hydrolyses. Enzymatic hydrolysis of agar and carrageenan into oligosaccharides is preferred in industrial production because of certain problems associated with chemical hydrolysis, including the release of high amounts of monosaccharides and undesirable toxic products, such as furfural. AOS and COS possess many biological activities, including prebiotic, immuno-modulatory, anti-oxidant, and anti-tumor activities. These activities are related to their chemical structure, molecular weight, degree of polymerization, and the flexibility of the glycosidic linkages. Therefore, the structure–function relationship and the mechanisms occurring during the specific biological applications of AOS and COS are discussed herein. Moreover, the chromatographic separation, purification, and characterization of AOS and COS are also part of this review. This piece of writing strives to create a new perspective on the potential applications of AOS and COS in the functional food and pharmaceutical industry.

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Section: Production Of Oligosaccharide From Red Seaweedmentioning

confidence: 99%

Section: Chromatographic Separation Of Oligosaccharidesmentioning

confidence: 99%

Oligosaccharides Derived from Red Seaweed: Production, Properties, and Potential Health and Cosmetic Applications

et al. 2018

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“…Agar oligosaccharide (AOS) is a small molecular oligosaccharide linked by repeated units of agarobiose, produced from agar polysaccharide. Recent researches show that AOS has good antioxidant activity and is easily absorbed (Chen et al ., ; Koti et al ., ; Higashimura et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterisation of a novel agar oligosaccharide–iron (III) complex

Teng

et al. 2018

Int J of Food Sci Tech

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This study aimed to synthesise a complex of agar oligosaccharides (AOS) with ferric iron and to evaluate its physicochemical and structural characteristics. Based on single factor, Plackett-Burman and response surface optimal experiments of three factors at three levels, the optimised chelate conditions of the AOSiron (III) complex were mass ratio of AOS to iron ions of 4.5:1 g:g, reaction temperature of 74°C and reaction time of 60 min. Under these conditions, the iron content and total sugar content were 14.03 AE 0.42% and 66.07 AE 0.96%, respectively. The physicochemical analysis showed that AOS-iron (III) was soluble and steady at physiological pH, and it was easily reduced to iron (II). The structural characteristics of AOS and AOS-iron (III) were analysed by Raman spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction and Nuclear magnetic resonance, and results showed that the iron (III) ions were chelated to AOS by -OH groups and -COOH groups and widely dispersed on the AOS backbone. Therefore, AOS-iron (III) could be a candidate for iron supplementation to treat iron deficiency anaemia.

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“…NeoDP4 is also known to alleviate the inflammatory response by inhibiting the MAPK and NF-κB signaling pathways [ 26 ]. Therefore, the requirement for production of NAOSs, especially NeoDP4, is increasing rapidly [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Production of neoagarooligosaccharides by probiotic yeast Saccharomyces cerevisiae var. boulardii engineered as a microbial cell factory

Jin

Liu

et al. 2021

Microb Cell Fact

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Background Saccharomyces cerevisiae var. boulardii is a representative probiotic yeast that has been widely used in the food and pharmaceutical industries. However, S. boulardii has not been studied as a microbial cell factory for producing useful substances. Agarose, a major component of red macroalgae, can be depolymerized into neoagarooligosaccharides (NAOSs) by an endo-type β-agarase. NAOSs, including neoagarotetraose (NeoDP4), are known to be health-benefiting substances owing to their prebiotic effect. Thus, NAOS production in the gut is required. In this study, the probiotic yeast S. boulardii was engineered to produce NAOSs by expressing an endo-type β-agarase, BpGH16A, derived from a human gut bacterium Bacteroides plebeius. Results In total, four different signal peptides were compared in S. boulardii for protein (BpGH16A) secretion for the first time. The SED1 signal peptide derived from Saccharomyces cerevisiae was selected as optimal for extracellular production of NeoDP4 from agarose. Expression of BpGH16A was performed in two ways using the plasmid vector system and the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system. The production of NeoDP4 by engineered S. boulardii was verified and quantified. NeoDP4 was produced by S. boulardii engineered using the plasmid vector system and CRISPR-Cas9 at 1.86 and 0.80 g/L in a 72-h fermentation, respectively. Conclusions This is the first report on NAOS production using the probiotic yeast S. boulardii. Our results suggest that S. boulardii can be considered a microbial cell factory to produce health-beneficial substances in the human gut. Graphical abstract

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Repeated batch production of agar-oligosaccharides from agarose by an amberlite IRA-900 immobilized agarase system

Cited by 15 publications

References 24 publications

Oligosaccharides Derived from Red Seaweed: Production, Properties, and Potential Health and Cosmetic Applications

Oligosaccharides Derived from Red Seaweed: Production, Properties, and Potential Health and Cosmetic Applications

Preparation and characterisation of a novel agar oligosaccharide–iron (III) complex

Production of neoagarooligosaccharides by probiotic yeast Saccharomyces cerevisiae var. boulardii engineered as a microbial cell factory

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