Repeated Cultured Epidermal Allografts in the Treatment of Chronic Leg Ulcers of Various Origins

Beele, Hilde; Naeyaert, Jean; Goeteyn, Marleen; Mil, Martine De; Kint, A

doi:10.1159/000247628

Cited by 56 publications

(45 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The animal assay reproduces experimentally the faster reepithelialization previously observed in human burns and leg ulcers treated with cultured human epidermal allogeneic sheets (Bolívar-Flores et al 1990;Nuñez-Gutiérrez et al 1996;Rivas-Torres et al 1996;De Luca, et al 1992;Beele et al 1991;Phillips et al 1989). Frozen epidermal cultures accelerated wound closure of full-thickness wounds in the mice by the migration and proliferation of the adjacent murine epidermal cells, and by formation of granulation tissue.…”

Section: Discussionsupporting

confidence: 53%

“…The cultured sheets applied to humans for the treatment of partial-thickness burns and leg ulcers are displaced and lost from the wound bed during the healing process (Bolívar-Flores et al 1990;Rivas-Torres et al 1996;De Luca et al 1992;Beele et al 1991;Phillips et al 1989). In mice, the cultured human epidermal sheet could be identified over the wound bed for no more than 4-6 days.…”

Section: Discussionmentioning

confidence: 99%

“…Their use for treatment of burned patients has led to an increased survival rate and reduced costs (Nuñez-Gutiérrez et al 1996). Keratinocyte cultures have also promoted reduction in healing time of leg ulcers (De Luca, et al 1992;Beele et al 1991;Phillips et al 1989).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Frozen cultured sheets of human epidermal keratinocytes enhance healing of full-thickness wounds in mice

Tamaríz

Marsch-Moreno

Castro‐Muñozledo

et al. 1999

Cell and Tissue Research

View full text Add to dashboard Cite

Sheets of cultured allogeneic human keratinocytes have been used for the treatment of burns and chronic leg ulcers but there has been no animal assay for the therapeutic action of these cultures. In order to analyze the effects of frozen cultures of human keratinocytes on wound healing, we have developed such an assay based on the rate of repair of full-thickness skin wounds in immunocompetent NMR1 mice. Reepithelialization of the control wounds, originating from the murine epithelium at the edge of the wound, occurred at a constant rate of advance of 150 microm/day. When frozen cultured human epidermal sheets were thawed at room temperature for 5-10 min and applied to the surface of the wound, the murine epithelium advanced at 267 microm/day. Most wounds treated with frozen cultures completely healed after 10 days, whereas most control wounds required 16 days. The accelerated reepithelialization did not depend on the presence of proliferative human keratinocytes in the frozen cultures. The cultures also promoted early formation of granulation tissue and laminin deposition over the surface of the wound bed. This simple assay should permit quantitative analysis of the effects on healing exerted not only by cultured cells, but also by proteins and small molecules.

show abstract

Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Frozen cultured sheets of human epidermal keratinocytes enhance healing of full-thickness wounds in mice

Tamaríz

Marsch-Moreno

Castro‐Muñozledo

et al. 1999

Cell and Tissue Research

View full text Add to dashboard Cite

show abstract

“…In 10 patients, epithelialization of 71 ±29% of the ulcer surface was noted at Day 30, but in no case was there any "graft take" in the center of the ulcer. Beele et al [66] reported complete closure of 62% of 16 leg ulcers within eight weeks after repeated application of allogeneic multilayered keratinocyte sheets stapled to petrolatum gauze. A mean of three applications were applied.…”

Section: Allogeneic Cell Approachesmentioning

confidence: 99%

Human cell therapy for venous leg ulcers

Slade¹,

Jacobson²,

Lu³

2015

View full text Add to dashboard Cite

Non-healing, chronic venous leg ulcers are sometimes mistakenly described as acute wounds that progress from hemostasis to inflammation but then become stuck in the inflammatory phase of healing. Typical explanations for this include colonization by bacteria, tissue infection, or the formation of bacterial biofilms. When standard care with debridement and compression bandaging fails to promote healing, therapeutic approaches shift to more aggressive surgical debridement, antimicrobial therapy, and the use of biological dressings or autologous grafts. A pathophysiologic view of persistent venous hypertension reveals the primary role of dermal inflammation, leading eventually to skin ulceration in a manner distinctly different from an acute wound. Subsequent to ulceration, additional inflammatory responses to invading pathogens can become important, but the underlying inflammatory state must be controlled before healing can take place, even with the use of skin grafts or cultured autologous skin cells. The prior belief that allogeneic keratinocytes and fibroblasts could engraft has given way to a more sophisticated consideration of the immunologic interplay between inflamed tissue and applied cells. This review briefly summarizes the history of cell therapy for venous leg ulcers, with a focus on concepts that have been tested in clinical trials, and a consideration of future development approaches.

show abstract

“…Im Vergleich hierzu lag die Heilungsrate in einer kontrollierten Studie von Poskitt et al [17] bei 46% nach Schweinehauttransplantation und 74% nach Transplantation kleiner Hautinseln. Hohe Heilungsraten konnten durch Deckung mit autologen Keratinozyten erzielt werden [1]. Auff~illig war hier die starke Epithelialisierung am Ulkusrand.…”

Section: Diagnostikunclassified

Ulcus cruris venosum: chirurgisches debridement, antibiotische therapie und stimulation mit thrombozyt�ren wachstumsfaktoren

Coerper

Köveker

Flesch

et al. 1995

Langenbecks Arch Chir

View full text Add to dashboard Cite

Despite the availability of various topical agents and of new technics for surgical correction, venous stasis ulcers are still characterized by high recurrent rates. Experimental data from wound healing studies demonstrate stimulation of wound healing after topical application of various growth factors (TGF beta, PDGF, EGF). The results of clinical studies suggest that topical use of an autologous platelet releasate (PDWHF) containing various growth factors accelerates healing. In this prospective study the stimulating effect of autologous PDWHF on epithelialization of small ulcers (group A, < 5000 mm2) and granulation of large ulcers before mesh grafting (> 5000 mm2) will be demonstrated. Inclusion criteria were the venous aetiology of the ulcer and the failure of conventional therapy for 6 month. Exclusion criteria were arterial occlusive disease, diabetes mellitus, acute wound infection, thrombocytopenia and pregnancy. There were 24 patients with 36 ulcers, caused by postthrombotic syndrome in one-third of cases and in two-thirds by severe insufficiency of the perforating veins. The ulcer had been present for more than in 10 years in 38% of cases, while there were 6 circumferential ulcers. The overall ulcer healing rate was 77% after a mean of 14 weeks. In group A 78% of the patients were healed after a mean of 16 weeks. In group B the mesh graft procedure was successful in 90% of the patients after a mean of 13 weeks. Compared with other conventional therapy studies, we achieved a higher healing rate. PDWHF seems to create ideal granulation tissue for mesh graft, indicated by a high uptake of the skin grafts.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Repeated Cultured Epidermal Allografts in the Treatment of Chronic Leg Ulcers of Various Origins

Cited by 56 publications

References 9 publications

Frozen cultured sheets of human epidermal keratinocytes enhance healing of full-thickness wounds in mice

Frozen cultured sheets of human epidermal keratinocytes enhance healing of full-thickness wounds in mice

Human cell therapy for venous leg ulcers

Ulcus cruris venosum: chirurgisches debridement, antibiotische therapie und stimulation mit thrombozyt�ren wachstumsfaktoren

Contact Info

Product

Resources

About