Repeated-doses and reproductive toxicity studies of the monoterpene 1,8-cineole (eucalyptol) in Wistar rats

Caldas, Germana Freire Rocha; Limeira, Mayara Marília Ferreira; Araújo, Alice V.; Albuquerque, Giwellington Silva; Neto, Jacinto da Costa Silva; Silva, Teresinha Gonçalves da; Costa‐Silva, João Henrique; Menezes, Irwin Rose Alencar de; Costa, José Galberto Martins da; Wanderley, Almir Gonçalves

doi:10.1016/j.fct.2016.09.020

Cited by 43 publications

(20 citation statements)

References 29 publications

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“…Blood samples were obtained by cardiac puncture using a syringe and collected in two tubes, one tube containing anticoagulant ethylenediaminetetraacetic acid (EDTA) and one tube without additional anticoagulant, for hematological and biochemical analyses, respectively. After that, the animals were euthanized for necropsy and evaluation of vital organs [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.

Brito

Almeida

Santana

et al. 2018

Oxidative Medicine and Cellular Longevity

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Spondias mombin L. is used in folk medicine for the treatment of inflammation and gastrointestinal diseases. Our study investigated the antiulcer activity of S. mombin ethanolic extract (SmEE) and its majority compounds gallic acid (GA) and ellagic acid (EA). Phytochemical characterization was performed by HPLC. The SmEE was screened for in vitro antioxidant activities using phosphomolybdenum, ABTS, DPPH, and FRAP assays. The antiulcer activity of SmEE, GA, EA, or GA + EA was evaluated by gastric lesion models induced by absolute ethanol and indomethacin. Following this, it is capable of stimulating mucus production, antisecretory capacity, and the influence of −SH groups and NO in the effect of SmEE. Its healing activity was demonstrated by acetic acid-induced chronic ulcer model. Anti-Helicobacter pylori activity was assessed by determining the MIC of the SmEE (64–1024 μg/mL). The HPLC results identified the presence of gallic acid and ellagic acid in SmEE. The extract showed antioxidant activity in vitro. SmEE (50, 100, and 200 mg/kg) reduced the area of ulcerative lesions induced by ethanol in 23.8, 90.3, and 90.2%, respectively. In NSAID model, the SmEE induced protection of 36.8, 49.4, and 49.9%, respectively. GA (10 mg/kg) or EA (7 mg/kg) or the association of GA + EA (10 + 7 mg/kg) inhibited the ethanol-induced lesions in 71.8, 70.9, and 94.9%, respectively, indicating synergistic action. SmEE (100 mg/kg) decreased acid secretion and H+ concentration in the gastric contents, increased levels of mucus, and showed to be dependent of −SH groups and NO on the protection of the gastric mucosa. In chronic ulcer model, SmEE reduced the gastric area lesion. SmEE showed anti-H. pylori activity. In conclusion, our study showed that SmEE has antiulcerogenic activity. GA and EA are isolated gastric protectors and, when associated, acted synergistically to protect the gastric mucosa.

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Section: Methodsmentioning

confidence: 99%

Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.

Brito

Almeida

Santana

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Regarding the toxicity of eucalyptol, Caldas et al (2016) recently estimated the LD50 between 1.500 and 1.750 mg/ kg in mice. They also provided evidence that possibly eucalyptol presents maternal and fetal toxicity and the need of more detailed investigation to better characterize the toxic effects of this monoterpene.…”

Section: Introductionmentioning

confidence: 99%

Acute and neuropathic orofacial antinociceptive effect of eucalyptol

et al. 2017

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Terpenes have a wide range of pharmacological properties, including antinociceptive action. The anti-inflammatory and antinociceptive effects of eucalyptol are well established. The purpose of this study was to evaluate the antinociceptive effect of eucalyptol on acute and neuropathic orofacial pain in rodent models. Acute orofacial and corneal nociception was induced with formalin, capsaicin, glutamate and hypertonic saline in mice. In another series, animals were pretreated with capsazepine or ruthenium red to evaluate the involvement of TRPV1 receptors in the effect of eucalyptol. In a separate experiment, perinasal tissue levels of IL-1β, TNF-α and IFN-γ were measured. Rats were pretreated with eucalyptol before induction of temporomandibular joint pain with formalin or mustard oil. In another experiment, rats were submitted to infraorbital nerve transection (IONX) to induce chronic pain, followed by induction of mechanical hypersensitivity using Von Frey hairs. Locomotor performance was evaluated with the open-field test, and molecular docking was conducted on the TRPV1 channel. Pretreatment with eucalyptol significantly reduced formalin-induced nociceptive behaviors in all mouse strains, but response was more homogenous in the Swiss strain. Eucalyptol produced antinociceptive effects in all tests. The effect was sensitive to capsazepine but not to ruthenium red. Moreover, eucalyptol significantly reduced IFN-γ levels. Matching the results of the experiment in vivo, the docking study indicated an interaction between eucalyptol and TRPV1. No locomotor activity changes were observed. Our study shows that eucalyptol may be a clinically relevant aid in the treatment of orofacial pain, possibly by acting as a TRPV1 channel antagonist.

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“…It is used as food flavouring and as excipient in the pharmaceutical industry. However, this organic compound presents possibly maternal and foetal toxicity (Caldas et al, 2016).…”

Section: Monoterpenes Phenylpropanoids and Prenylflavonoidsmentioning

confidence: 99%

Wild edible plants: Nutritional and toxicological characteristics, retrieval strategies and importance for today's society

Pinela

Ferreira

2017

Food and Chemical Toxicology

146

111

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A B S T R A C TWild edible plants (WEPs) are part of the cultural and genetic heritage of different regions of the world. In times of famine and scarcity, these sources of nutrients and health-promoting compounds have received high importance mainly in rural and suburban areas. Although currently underutilized, WEPs are still consumed traditionally by different communities and are gaining relevance in today's society. However, these foods lack recognition as significant contributors to the human diet in developed areas. This review describes the nutritional value of WEPs from the North-eastern region of Portugal and points out those containing potentially toxic compounds. Several retrieval strategies are presented with the aim of promoting the (re)use, production, commercialization and conservation of WEPs (wild harvested plants and crop wild relatives), and their importance for social, economic and agro-ecological development is highlighted.

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Repeated-doses and reproductive toxicity studies of the monoterpene 1,8-cineole (eucalyptol) in Wistar rats

Cited by 43 publications

References 29 publications

Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.

Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.

Acute and neuropathic orofacial antinociceptive effect of eucalyptol

Wild edible plants: Nutritional and toxicological characteristics, retrieval strategies and importance for today's society

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