Repeated endoscopic ovum pick-up in sheep

Stangl, M; Kühholzer, B.; Besenfelder, U.; Brem, Gottfried

doi:10.1016/s0093-691x(99)00164-8

Cited by 54 publications

(60 citation statements)

References 16 publications

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“…1994). However, Stangl et al. (1999) reported similar oocyte recovery rates for ewes with and without FSH treatment, although differences in the technique and equipment used probably accounts for these different observations.…”

Section: Discussionmentioning

confidence: 94%

Repeat Ovum Pick‐up and In Vitro Embryo Production from Adult Ewes with and without FSH Treatment

Morton

Graaf

Campbell

et al. 2005

Reprod Domestic Animals

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Ovum pick-up (OPU) was performed three times on adult ewes after synchronization with (n = 4) or without (n = 4) FSH treatment to investigate the effects of FSH treatment on the number of ovarian follicles, oocytes recovered, oocyte quality and development in vitro. FSH treatment increased the number of ovarian follicles (85 vs 162) and oocytes recovered (33 vs 91), although recovery rate was similar for ewes with and without FSH (91/162, 56.2% and 33/85, 38.8% respectively). Of the oocytes recovered, those classified as grades I and II were similar between ewes with (78/91, 85.7%) and without FSH treatment (27/33, 81.8%). The number of ovarian follicles was similar after repeated OPU for ewes treated with FSH, but for ewes not treated with FSH the number of ovarian follicles decreased with repeated OPU. The number of oocytes recovered decreased for FSH-treated ewes only, while the oocyte recovery rate and proportion of oocytes classified as grades I and II were not affected by repeated OPU. Oocyte cleavage (46/78, 58.9% and 19/24, 79.2%) and blastocyst formation (35/46, 76.1% and 12/19, 63.2% respectively) were similar for ewes with and without FSH treatment. The number of ovarian follicles varied between ewes (p < 0.05) although the number of oocytes recovered and oocyte development in vitro were similar between ewes.

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Section: Discussionmentioning

confidence: 94%

Repeat Ovum Pick‐up and In Vitro Embryo Production from Adult Ewes with and without FSH Treatment

Morton

Graaf

Campbell

et al. 2005

Reprod Domestic Animals

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“…When eCG binding was higher than 10%, the percentage of late estrus was high and the fertility after AI at a predetermined time decreased significantly. The use of eCG was also reported in OPU protocols in sheep [36].…”

Section: Radiolabeling With 125 Iodinementioning

confidence: 94%

Increase of plasma eCG binding rate after administration of repeated high dose of eCG to cows

Drion

Roover²,

Houtain³

et al. 2001

Reprod. Nutr. Dev.

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-Equine chorionic gonadotrophin (eCG) is still used to promote follicular growth in cattle and, more recently with an increased frequency of administration, in ovum pick-up protocols. The aim of this experiment was to verify the possible effect of high frequency of administration on the immune response to eCG. The profiles of eCG binding rate, in the blood of two groups (A, B) of 4 primiparous cross breed beef cows (3-3.5 years old) submitted weekly for 5 to 10 weeks to repeated high doses (1 000-2 000 IU) of equine chorionic gonadotrophin, are presented in this paper. A sensitive radiometric method was used to detect antibodies in plasma. The profiles clearly indicated a marked increase of eCG binding rate after 3 to 5 injections of the exogenous hormone to the females. The statistical analysis of the results established that treatments induced a significant increase (P < 0.01) in binding rates after 6 and 3 injections in group A and B respectively. These binding rates remained elevated for at least 1 week following the last injection and decreased afterwards. The values of plasma binding rates following repeated eCG administration differed significantly between groups (0.90 ± 1.04 and 1.04 ± 0.11 for groups A and B before treatment versus 11.77 ± 0.92, 6.70 ± 0.85 for groups A and B after treatment, P < 0.01) and from one cow to another (P < 0.01) with some cows presenting no significant immune response while others were more reactive against the hormone (at least 3 injections).cow / eCG / antibody Reprod. Nutr. Dev. 41 (2001) 207-215 207

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“…The initial target was the collection of oocytes from valuable females of species where ultrasound-guided ovum pick-up (OPU) was very difficult or not possible, due to animal size issues. In that sense, LOPU rapidly became, and remains as, the method of choice for the collection of immature oocytes for IVEP in sheep and goats (Baldassarre et al, 1994;Stangl et al, 1999;Baldassarre et al, 2002;Cognie et al, 2004). Moreover, LOPU-IVEP has also been applied for oocyte collection in wild life species including deer and big felines (Miller et al, 1990;Locatelli et al, 2006;Baldassarre et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Baldassarre

Bordignon

2018

Anim Reprod

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Laparoscopic ovum pick-up (LOPU) conducted on bovine and buffalo calves of 2-6-month of age, followed by in vitro embryo production and transfer into synchronous adult recipients, is a powerful tool for accelerated genetic gain and early dissemination of top genetics. In its current state, the technology is characterized by higher oocyte recovery rates, lower oocyte-to-embryo yields, and similar pregnancy and term development rates compared with adult counterparts. Improvements in oocyte competence have been made in recent years mainly through gonadotropin stimulation protocols tailored for prepubertal donors. These advances have brought the technology to the point of been apt for commercial application. However, future research must focus on increasing the proportion of fully competent oocytes recovered from calves thereby further empowering the role this technology platform can play in programs for accelerated dissemination of superior genetics.

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Repeated endoscopic ovum pick-up in sheep

Cited by 54 publications

References 16 publications

Repeat Ovum Pick‐up and In Vitro Embryo Production from Adult Ewes with and without FSH Treatment

Repeat Ovum Pick‐up and In Vitro Embryo Production from Adult Ewes with and without FSH Treatment

Increase of plasma eCG binding rate after administration of repeated high dose of eCG to cows

Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

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