Repeated ethanol production from sweet sorghum juice concentrated by membrane separation

Sasaki, Kengo; Tsuge, Yota; Sasaki, Daisuke; Kawaguchi, Hideo; Sazuka, Takashi; Ogino, Chiaki; Kondo, Akihiko

doi:10.1016/j.biortech.2015.03.127

Cited by 22 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…43 Repeated use of cells for multiple production cycles reduces the costs and time of downstream processing. 44,45 The use of an oxygen-responsive metabolic switch demonstrated in the current study offers a new approach for an efficient production of compounds for improved titer and yield, while also saving the production costs and time by allowing a repeated use of cells for aerobic and anaerobic production.…”

Section: Modulation Of the Split Ratio Of Carbon Flux Between Glycoly...mentioning

confidence: 98%

Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

et al. 2020

Self Cite

View full text Add to dashboard Cite

Controlling the carbon flux into a desired pathway is important for improving product yield in metabolic engineering. After entering a cell, glucose is channeled into glycolysis and the pentose phosphate pathway (PPP), which decreases the yield of target products whose synthesis relies on NADPH as a cofactor. Here, we demonstrate redirection of carbon flux into PPP under aerobic conditions in Corynebacterium glutamicum, achieved by replacing the promoter of glucose 6-phosphate isomerase gene (pgi) with an anaerobic-specific promoter of the lactate dehydrogenase gene (ldhA). The promoter replacement increased the split ratio of carbon flux into PPP from 39 to 83% under aerobic conditions. The titer, yield, and production rate of 1,5-diaminopentane, whose synthesis requires NADPH as a cofactor, were increased by 4.6-, 4.4-, and 2.6-fold, respectively. This is the largest improvement in the production of 1,5-diaminopentane or its precursor, lysine, reported to date. After aerobic cell growth, pgi expression was automatically induced under anaerobic conditions, altering the carbon flux from PPP to glycolysis, to produce succinate in a single metabolically engineered strain. Such an automatic redirection of metabolic pathway using an oxygen-responsive switch enables two-stage fermentation for efficient production of two different compounds by a single strain, potentially reducing the production costs and time for practical applications.

show abstract

Section: Modulation Of the Split Ratio Of Carbon Flux Between Glycoly...mentioning

confidence: 98%

Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sequential batch fermentation from sweet sorghum juice concentrated by membrane separation to increase sugar contents, was investigated by Sasaki et al, (2015). Increase HCl was more helpful in converting the cellulosic materials to reducing sugars.…”

Section: Sweetmentioning

confidence: 99%

Bioenergy from Biofuel Residues and Wastes

Choudri

Baawain

2016

Water Environment Research

View full text Add to dashboard Cite

This review includes works published in the general scientific literature during 2015 on the production of bioenergy and biofuel from waste residues generated during bioethanol and biodiesel production with a brief overview of current and emerging feedstocks. A section of this review summarizes literature on culturing algae for biofuels including bioreactors and open pond cultivation systems with the utilization of inorganic and organic sources of nutrients. New methods applicable to the mass culture of algae are highlighted. Algal cell harvesting and oil extraction techniques tested and developed for algae discussed alongwith policies and economics are also provided.

show abstract

“…Issatchenkia orientalis [222][223][224] Sweet Sorghum juice Saccharomyces cerevisiae [225] Paddy straw Trichoderma ressei [226] Sugarcane baggase Trichodermaand Penicillium Saccharomyces cerevisiae [227] Ulva fasciata Cladosporium sphaerospermum [228] Food waste Myceliophthora thermophila Saccharomyces cerevisiae [229] Ziziphus jujuba Saccharomyces bayanus [230] to disassociation of each group and make the compound resist for long or sometimes almost as undegradable compound. Though, the application of enzymes to waste treatment was proposed in 1930s [62], was first illustrated in the late 1970s through degradation of parathion using enzyme [63].…”

Section: Saccharomyces Cerevisiaementioning

confidence: 99%

Solid State Fermentation: Comprehensive Tool for Utilization of Lignocellulosic through Biotechnology

Koyani¹,

Rajput²

2015

J Bioprocess Biotech

View full text Add to dashboard Cite

Repeated ethanol production from sweet sorghum juice concentrated by membrane separation

Cited by 22 publications

References 17 publications

Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

Bioenergy from Biofuel Residues and Wastes

Solid State Fermentation: Comprehensive Tool for Utilization of Lignocellulosic through Biotechnology

Contact Info

Product

Resources

About