Repeated fed‐batch production of xylitol by <i>Candida magnoliae</i><scp>TISTR</scp> 5663

Sirisansaneeyakul, Sarote; Wannawilai, Siwaporn; Chisti, Yusuf

doi:10.1002/jctb.3949

Cited by 30 publications

(7 citation statements)

References 29 publications

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“…The specific and volumetric rates for growth, the consumption of nitrogen and phosphorus, and the formation of products were calculated as described by Sirisansaneeyakul et al …”

Section: Methodsmentioning

confidence: 99%

Simultaneous production of C‐phycocyanin and extracellular polymeric substances by photoautotrophic cultures of Arthrospira platensis

Dejsungkranont

Chisti

Sirisansaneeyakul

2017

J of Chemical Tech & Biotech

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BACKGROUND: Arthrospira platensis produces the blue pigment C-phycocyanin (C-PC) and extracellular polymeric substances (EPS) of potential value in food and pharmaceutical applications. RESULTS: Batch cultures were used to simultaneously produce C-PC and EPS. The effects of incident light level on growth and product formation are reported. A maximum biomass concentration of 1.5 g L −1 in 14 days was obtained at a light intensity of 203 mol m −2 s −1 . The light intensity affected the biomass composition and the production of the two products. Protein, phenolic compounds and chlorophyll a in the biomass were reduced at high light levels, but carbohydrate and the total carotenoids were increased. Production of C-PC was growth associated. The highest concentration of C-PC in the culture broth was ∼60 mg L −1 at a photon flux of 135 mol m −2 s −1 . EPS was produced mostly in the stationary phase. The highest EPS concentration of ∼960 mg L −1 occurred at a photon flux of 203 mol m −2 s −1 . Incident light level also affected the antioxidant properties of the two products. CONCLUSION: Incident light strongly affected the production of C-PC, EPS and the biomass composition. Low light favored C-PC production whereas high light favored the production of EPS.

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“…The specific and volumetric rates for growth, the consumption of nitrogen and phosphorus, and the formation of products were calculated as described by Sirisansaneeyakul et al …”

Section: Methodsmentioning

confidence: 99%

Simultaneous production of C‐phycocyanin and extracellular polymeric substances by photoautotrophic cultures of Arthrospira platensis

Dejsungkranont

Chisti

Sirisansaneeyakul

2017

J of Chemical Tech & Biotech

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“…The fermentation kinetic parameters were calculated according to Sirisansaneeyakul and others (). The kinetic parameters were calculated on a specific volume basis to account for the changes in volume caused by sampling.…”

Section: Methodsmentioning

confidence: 99%

Fungal Isolates from a Pu‐Erh Type Tea Fermentation and Their Ability to Convert Tea Polyphenols to Theabrownins

Wang

Gong

Chisti

et al. 2015

Journal of Food Science

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The natural microbiota involved in the fermentation influence the quality and taste of fully postfermented teas such as China's Pu-erh tea. Ten microbial isolates representing 6 species were recovered from a solid-state fermentation of a Pu-erh type tea. The isolates were Aspergillus tubingensis, Aspergillus marvanovae, Rhizomucor pusillus, Rhizomucor tauricus, Aspergillus fumigatus, and Candida mogii. With the exception of A. marvanovae and C. mogii, all these microorganisms have been previously reported in solid-state fermentations of native Pu-erh tea. The ability of the isolates for converting the tea polyphenols to bioactive theabrownins in infusions of sun-dried green tea leaves in a submerged fermentation process was subsequently investigated. All isolates except C. mogii TISTR 5938 effectively produced theabrownins in a 4-d fermentation in shake flasks at 40 °C, 250 rpm. A. tubingensis TISTR 3646, A. tubingensis TISTR 3647, A. marvanovae TISTR 3648, and A. fumigatus TISTR 3654 produced theabrownins at particularly high levels of 6.5, 12.4, 11.1, and 8.4 g/L, respectively.

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“…was increased by 27.84% at 80% medium replacement ratio compared with 95% in repeated fed-batch culture [12]. However, 50% medium replacement ratio was found to be the most suitable for the production of cellulase by Penicillium oxalicum RE-10 [15], and 50% medium replacement ratio was also used to achieve the best production of xylitol by Candida magnoliae TISTR 5663 [30]. One of the possible reasons for the difference in the optimal medium replacement ratio might be related to the types of microbes and products [15,31].…”

Section: Effects Of Medium Replacement Ratio On Dha Production In Repmentioning

confidence: 99%

Repeated fed-batch strategy and metabolomic analysis to achieve high docosahexaenoic acid productivity in Crypthecodinium cohnii

et al. 2020

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Background: Docosahexaenoic acid (DHA) is essential for human diet. However, high production cost of DHA using C. cohnii makes it currently less competitive commercially, which is mainly caused by low DHA productivity. In recent years, repeated fed-batch strategies have been evaluated for increasing the production of many fermentation products. The reduction in terms of stability of culture system was one of the major challenges for repeated fed-batch fermentation. However, the possible mechanisms responsible for the decreased stability of the culture system in the repeated fedbatch fermentation are so far less investigated, restricting the efforts to further improve the productivity. In this study, a repeated fed-batch strategy for DHA production using C. cohnii M-1-2 was evaluated to improve DHA productivity and reduce production cost, and then the underlying mechanisms related to the gradually decreased stability of the culture system in repeated fed-batch culture were explored through LC-and GC-MS metabolomic analyses. Results: It was discovered that glucose concentration at 15-27 g/L and 80% medium replacement ratio were suitable for the growth of C. cohnii M-1-2 during the repeated fed-batch culture. A four-cycle repeated fed-batch culture was successfully developed and assessed at the optimum cultivation parameters, resulting in increasing the total DHA productivity by 26.28% compared with the highest DHA productivity of 57.08 mg/L/h reported using C. cohnii, including the time required for preparing seed culture and fermentor. In addition, LC-and GC-MS metabolomics analyses showed that the gradually decreased nitrogen utilization capacity, and down-regulated glycolysis and TCA cycle were correlated with the decreased stability of the culture system during the long-time repeated fed-batch culture. At last, some biomarkers, such as Pyr, Cit, OXA, FUM, l-tryptophan, l-threonine, l-leucine, serotonin, and 4-guanidinobutyric acid, correlated with the stability of culture system of C. cohnii M-1-2 were identified. Conclusions: The study proved that repeated fed-batch cultivation was an efficient and energy-saving strategy for industrial production of DHA using C. cohnii, which could also be useful for cultivation of other microbes to improve productivity and reduce production cost. In addition, the mechanisms study at metabolite level can also be useful to further optimize production processes for C. cohnii and other microbes.

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Repeated fed‐batch production of xylitol by Candida magnoliaeTISTR 5663

Abstract: BACKGROUND: Xylitol is an important commercial sweetener that can be produced by fermentation. Previous studies of xylitol production have not been able to combine a high final product concentration, and a high average productivity and yield, in a single process.

Cited by 30 publications

References 29 publications

Simultaneous production of C‐phycocyanin and extracellular polymeric substances by photoautotrophic cultures of Arthrospira platensis

Simultaneous production of C‐phycocyanin and extracellular polymeric substances by photoautotrophic cultures of Arthrospira platensis

Fungal Isolates from a Pu‐Erh Type Tea Fermentation and Their Ability to Convert Tea Polyphenols to Theabrownins

Repeated fed-batch strategy and metabolomic analysis to achieve high docosahexaenoic acid productivity in Crypthecodinium cohnii

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