Reperfusion Injury and Renal Metabolism : The Temporal Relationship Between Oxidative Stress and Functional Change

Fuller, B; Gower, Jon D.; Cotterill, L; Healing, Guy; Simpkin, S.; Green, C. J.

doi:10.1007/978-1-4684-5730-8_61

Cited by 4 publications

(1 citation statement)

References 2 publications

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“…The most critical question that arises from these data is how MMF reduces renal injury as the result of renal injury markers, such as NGAL and L‐FABP, without affecting lipid peroxidation (no change in MDA), particularly in light of the putative contention that I/R‐induced lipid peroxidation is detrimental to organ viability . This study also showed that there were no notable differences in blood gas and electrolyte levels following lower body ischaemia/reperfusion, except for a lower pH and higher PaCO 2 in the I/R and I/R+MMF groups, respectively.…”

Section: Discussionmentioning

confidence: 69%

Mycophenolate mofetil improves renal haemodynamics, microvascular oxygenation, and inflammation in a rat model of supra‐renal aortic clamping‐mediated renal ischaemia reperfusion injury

Ergin

Heger

Kandil

et al. 2017

Clin Exp Pharma Physio

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Ischaemia/reperfusion (I/R) is one of the main causes of acute kidney injury (AKI), which is characterized by sterile inflammation and oxidative stress. Immune cell activation can provoke overproduction of inflammatory mediators and reactive oxygen species (ROS), leading to perturbation of the microcirculation and tissue oxygenation associated with local and remote tissue injury. This study investigated whether the clinically employed immunosuppressant mycophenolate mofetil (MMF) was able to reduce I/R-induced renal oxygenation defects and oxidative stress by preventing sterile inflammation. Rats were divided into three groups (n=6/group): (1) a sham-operated control group; (2) a group subjected to renal I/R alone (I/R); and (3) a group subjected to I/R and MMF treatment (20 mg/kg prior to I/R) (I/R+MMF). Ischaemia was induced by a vascular occluder placed on the abdominal aorta for 30 minutes, followed by 120 minutes of reperfusion. Renal I/R deteriorated renal oxygenation (P<.001) and oxygen delivery (P<.01), reduced creatinine clearance (P<.01) and tubular sodium reabsorption (P<.001), and increased iNOS, renal tissue injury markers (P<.001), and IL-6 (P<.001). Oral MMF administration prior to insult restored renal cortical oxygenation (P<.05) and iNOS, renal injury markers, and inflammation parameters (P<.001) to near-baseline levels without affecting renal function. MMF exerted a prophylactic effect on renal microvascular oxygenation and abrogated tissue inflammation and renal injury following lower body I/R-induced AKI. These findings may have clinical implications during major vascular or renal transplant surgery.

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Section: Discussionmentioning

confidence: 69%