Repetitive CREB-DNA interactions at gene loci predetermined by CBP induce activity-dependent gene expression in human cortical neurons

Atsumi, Yuri; Iwata, Ryohei; Kimura, Hiroshi; Vanderhaeghen, Pierre; Yamamoto, Nobuhiko; Sugo, Noriyuki

doi:10.1016/j.celrep.2023.113576

Cited by 4 publications

(3 citation statements)

References 80 publications

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“…KCl-induced depolarization has often been used as a model to investigate the effect of neuronal activity in culture [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. To examine whether the phosphorylation status of the tau protein is affected by KCl-induced depolarization, we generated a mouse hippocampal neuron primary culture and performed KCl stimulation.…”

Section: Resultsmentioning

confidence: 99%

“…of neuronal activity in culture [8][9][10][11][12][13][14][15]. To examine whether the phosphorylation status of the tau protein is affected by KCl-induced depolarization, we generated a mouse hippocampal neuron primary culture and performed KCl stimulation.…”

Section: Kcl Treatment Led To Tau Protein Dephosphorylation In Primar...mentioning

confidence: 99%

“…In studies using cultured neurons, KCl-induced depolarization is often used as a means of generating electrical excitation to mimic neuronal physiological activity [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. Previous studies have shown that the depolarization of cultured neurons induced by KCl leads to tau protein dephosphorylation [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Neuronal Excitation Induces Tau Protein Dephosphorylation via Protein Phosphatase 1 Activation to Promote Its Binding with Stable Microtubules

Yagishita,

Shibata,

Furuno

et al. 2024

Neurology International

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The tau protein is a microtubule-associated protein that promotes microtubule stabilization. The phosphorylation of the tau protein has been linked to its dissociation from microtubules. Here, we examined the relationship between neuronal depolarization activity and tau protein phosphorylation by employing model systems in culture as well as in vivo. The KCl-evoked depolarization of cultured neurons has often been used to investigate the effects of neuronal activity. We found dephosphorylation at AT8 sites (S202, T205), T212, AT180 sites (T231, S235), and S396 in KCl-simulated cultured neurons. We also found that the KCl-induced tau protein dephosphorylation increases the level of the tau protein fractionated with stable microtubules. In an in vivo experiment, we demonstrated that the exposure of mice to a new environment activates protein phosphatase 1 in the mouse hippocampus and induces tau protein dephosphorylation. We also found an increased amount of the tau protein in a stable microtubule fraction, suggesting that the dephosphorylation of the tau protein may lead to its increased microtubule association in vivo. These results suggest that the association of microtubules with tau proteins may be regulated by the tau protein phosphorylation status affected by neuronal electrical activity.

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Section: Resultsmentioning

confidence: 99%

Section: Kcl Treatment Led To Tau Protein Dephosphorylation In Primar...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Neuronal Excitation Induces Tau Protein Dephosphorylation via Protein Phosphatase 1 Activation to Promote Its Binding with Stable Microtubules

Yagishita,

Shibata,

Furuno

et al. 2024

Neurology International

View full text Add to dashboard Cite

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Protocol for single-molecule imaging of transcription and epigenetic factors in human neural stem cell-derived neurons

Atsumi,

Yamamoto,

Sugo

2024

STAR Protocols

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Multi-faceted regulation of CREB family transcription factors

Chowdhury,

Haq,

Lee

et al. 2024

Front. Mol. Neurosci.

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cAMP response element-binding protein (CREB) is a ubiquitously expressed nuclear transcription factor, which can be constitutively activated regardless of external stimuli or be inducibly activated by external factors such as stressors, hormones, neurotransmitters, and growth factors. However, CREB controls diverse biological processes including cell growth, differentiation, proliferation, survival, apoptosis in a cell-type-specific manner. The diverse functions of CREB appear to be due to CREB-mediated differential gene expression that depends on cAMP response elements and multi-faceted regulation of CREB activity. Indeed, the transcriptional activity of CREB is controlled at several levels including alternative splicing, post-translational modification, dimerization, specific transcriptional co-activators, non-coding small RNAs, and epigenetic regulation. In this review, we present versatile regulatory modes of CREB family transcription factors and discuss their functional consequences.

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Repetitive CREB-DNA interactions at gene loci predetermined by CBP induce activity-dependent gene expression in human cortical neurons

Cited by 4 publications

References 80 publications

Neuronal Excitation Induces Tau Protein Dephosphorylation via Protein Phosphatase 1 Activation to Promote Its Binding with Stable Microtubules

Neuronal Excitation Induces Tau Protein Dephosphorylation via Protein Phosphatase 1 Activation to Promote Its Binding with Stable Microtubules

Protocol for single-molecule imaging of transcription and epigenetic factors in human neural stem cell-derived neurons

Multi-faceted regulation of CREB family transcription factors

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