Repetitive Fragmentation Products of Albumin and α1-Antitrypsin in Glomerular Diseases Associated with Nephrotic Syndrome

Candiano, Giovanni; Musante, Luca; Bruschi, Maurizio; Petretto, Andrea; Santucci, Laura; Boccio, Piero Del; Pavone, Barbara; Perfumo, Francesco; Urbani, Andrea; Scolari, Francesco; Ghiggeri, Gian Marco

doi:10.1681/asn.2006050486

Cited by 136 publications

(118 citation statements)

References 32 publications

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“…Many CSF albumin fragments have been observed in 2DE maps in different pathological conditions [9,14,5]. We found an extensive albumin fragmentation in our GBS CSF samples.…”

supporting

confidence: 55%

Protein profiling of Guillain–Barrè syndrome cerebrospinal fluid by two-dimensional electrophoresis and mass spectrometry

D’Aguanno

Franciotta

Lupisella

et al. 2010

Neuroscience Letters

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a b s t r a c tProtein profiling of cerebrospinal fluid in Guillain-Barrè syndrome (GBS), an acute and immune-mediated disease affecting the peripheral nervous system, was performed by two-dimensional electrophoresis. Significant modulated spots in GBS patients vs. control groups (a group of multiple sclerosis patients and one of healthy donors) underwent MALDI-TOF/TOF investigation. Inflammation-related proteins, such as vitamin D-binding protein, beta-2 glycoprotein I (ApoH), and a complement component C3 isoform were up-regulated in GBS, whereas transthyretin (the monomer and the dimer forms), apolipoprotein E, albumin and five of its fragments were down-regulated. Then, we used an isoelectric-focusingdinitrophenylhydrazine-based technique to analyse the extent of carbonylation and, as a result, of oxidative damage of GBS CSF proteome. We observed a major sensitivity to carbonylation for albumin and alpha-glycoprotein in inflammation and a selective increase of reactivity for a glycosylated Fab from an IgM globulin in GBS CSF. Our results add new proteins to candidate CSF features of GBS, and suggest that oxidative stress could contribute to the immunopathological mechanisms in this syndrome.

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“…Many CSF albumin fragments have been observed in 2DE maps in different pathological conditions [9,14,5]. We found an extensive albumin fragmentation in our GBS CSF samples.…”

supporting

confidence: 55%

Protein profiling of Guillain–Barrè syndrome cerebrospinal fluid by two-dimensional electrophoresis and mass spectrometry

D’Aguanno

Franciotta

Lupisella

et al. 2010

Neuroscience Letters

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“…3A) as they were detected earlier in a patient with membraneous glomerulonephritis [28]. For the analyzed sample, it cannot be excluded that the marathon runner also suffered from nephropathy although this would probably restrict the exercise performance.…”

Section: Individual Casesmentioning

confidence: 67%

Effects of endurance exercise on the urinary proteome analyzed by 2‐D PAGE and Orbitrap MS

Kohler

Walpurgis

Thomas

et al. 2010

Proteomics Clinical Apps

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Purpose: Exercise-induced proteinuria is a well-known phenomenon and the influence of parameters such as intensity and duration was studied extensively. Usually, total protein or albumin was measured for diagnosis of a proteinuria, and the present study was performed to search for qualitative differences in the urinary proteome before and after endurance exercise. Experimental design: Urine samples were concentrated and proteins separated by means of 2-D PAGE. Proteins differing in the investigated groups were identified by nano-UPLCOrbitrap MS after trypsin digestion. Results: The study yielded several proteins such as hemopexin, albumin, orosomucoid 1, transferrin or carbonic anhydrase 1 that were elevated after a marathon run in comparison to a control group. These are linked to physiological changes resulting from endurance exercise such as destruction of erythrocytes or increased fat metabolism. On the contrary, 2-D PAGE profiles of athletes at rest did not differ from those of control samples. Conclusions and clinical relevance:The study is a starting point to build up individual 2-D PAGE protein maps of athletes. Further studies will investigate intra-individual differences and further exercise parameters, which potentially lead to a physiological monitoring system for athletes in training and competition and may also complement the blood passport in doping control.

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“…Many "potential markers" identified in proteomic experiments have been from amongst the most abundant proteins (e.g. [18,19]) and whether these will prove to be robust and/or specific still needs to be determined. A strict requirement for sequence will favour reports on only these abundant proteins and therefore appears not advisable.…”

Section: Ideally All Peptide/protein Biomarkers Should Be Sequencedmentioning

confidence: 99%

Clinical proteomics: A need to define the field and to begin to set adequate standards

Mischak

Apweiler

Banks

et al. 2007

Proteomics Clinical Apps

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The aim of this manuscript is to initiate a constructive discussion about the definition of clinical proteomics, study requirements, pitfalls and (potential) use. Furthermore, we hope to stimulate proposals for the optimal use of future opportunities and seek unification of the approaches in clinical proteomic studies. We have outlined our collective views about the basic principles that should be considered in clinical proteomic studies, including sample selection, choice of technology and appropriate quality control, and the need for collaborative interdisciplinary efforts involving clinicians and scientists. Furthermore, we propose guidelines for the critical aspects that should be included in published reports. Our hope is that, as a result of stimulating discussion, a consensus will be reached amongst the scientific community leading to guidelines for the studies, similar to those already published for mass spectrometric sequencing data. We contend that clinical proteomics is not just a collection of studies dealing with analysis of clinical samples. Rather, the essence of clinical proteomics should be to address clinically relevant questions and to improve the state-of-the-art, both in diagnosis and in therapy of diseases.

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Repetitive Fragmentation Products of Albumin and α1-Antitrypsin in Glomerular Diseases Associated with Nephrotic Syndrome

Cited by 136 publications

References 32 publications

Protein profiling of Guillain–Barrè syndrome cerebrospinal fluid by two-dimensional electrophoresis and mass spectrometry

Protein profiling of Guillain–Barrè syndrome cerebrospinal fluid by two-dimensional electrophoresis and mass spectrometry

Effects of endurance exercise on the urinary proteome analyzed by 2‐D PAGE and Orbitrap MS

Clinical proteomics: A need to define the field and to begin to set adequate standards

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