Repetitive sequences in the ribosomal DNA internal transcribed spacer of Schistosoma haematobium, Schistosoma intercalatum and Schistosoma mattheei

Kane, Richard A.; Rollinson, David

doi:10.1016/0166-6851(94)90018-3

Cited by 140 publications

(95 citation statements)

References 20 publications

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“…Due to its conserved nature, the ITS2 region is a sensitive and reliable diagnostic marker at the species or genus level (Adlard et al 1993;Kane and Rollinson 1994;Blair et al 1997aBlair et al , 1997b. In comparison, the ITS1 region is far more variable and therefore useful for investigating intra-specific patterns of variation.…”

Section: Discussionmentioning

confidence: 99%

Use of ITS rDNA for discriminating of larval stages of two microphallid (Digenea) species using Hydrobia ulvae (Pennant, 1777) and Corophium volutator (Pallas, 1766) as intermediate hosts

et al. 2004

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Digenean trematodes encompass several species with little morphological differentiation in the larval stages and, as a result, uncertainty prevails regarding species identification. The microphallid trematode Maritrema subdolum occurs widespread geographically in mud snail and crustacean hosts in European marine shallow-water ecosystems. Larval stages of this and other congeneric species are, however, difficult to separate morphologically. To verify the species status and to secure identification of two co-occurring microphallids, M. subdolum and microphallid sp. no. 15 (Deblock, 1980), we examined the nucleotide sequences of the internal transcribed spacer regions (ITS1, ITS2). From fragments consisting of both ITS regions and the 5.8S gene (nearly 1,200 bp), a sequence divergence of 2.9% between the two types was recorded. In accordance with the morphological traits of the cercariae (stylet shape, length), the results support the view that the two types actually represent different species. Species-specific primers were prepared for each species. They proved to be efficient diagnostic tools for identifying single larvalstage individuals. Using these primers, infections in host organisms were also verified without performing a dissection of the host individuals.

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Section: Discussionmentioning

confidence: 99%

Use of ITS rDNA for discriminating of larval stages of two microphallid (Digenea) species using Hydrobia ulvae (Pennant, 1777) and Corophium volutator (Pallas, 1766) as intermediate hosts

et al. 2004

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“…The entire ITS region was amplified using the primers ETTS2 (5-TAACAAGGTTTCCGTAGGTGAA-3) and ETTS1 (5-TGCTTAAGTTCAGCGGGT-3) (Kane & Rollinson 1994). PCR-RFLP conditions were the same described by Vidigal et al (1998), with DdeI enzyme.…”

mentioning

confidence: 99%

Dominant character of the molecular marker of a Biomphalaria tenagophila strain (Mollusca: Planorbidae) resistant to Schistosoma mansoni

Rosa¹,

Caldeira

Carvalho

et al. 2004

Mem. Inst. Oswaldo Cruz

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Biomphalaria tenagophila is a planorbid with a wide distribution in South America (Paraense 1984), and has epidemiological importance, since this species maintains the cycle of the trematode Schistosoma mansoni in some areas in Brazil. In spite of its dominance in some areas, B. tenagophila is found in the nature with low rates of infection, however, it is responsible for the majority of the autochthonous cases of schistosomiasis in the state of São Paulo, as well as for the foci of the disease in the states of Minas Gerais and Santa Catarina (Paraense 1986). This last focus is maintained by a highly susceptible population (Joinville) of molluscs to S. mansoni. On the other hand, there is a population coming from the Ecological Reservoir from Taim (state of Rio Grande do Sul), that has systematically been found resistant to different strains of S. mansoni (Coelho 1995). This population has been maintained under laboratory conditions for more than twenty years, without any selective pressure favoring the resistance. Vidigal et al. (1998), using the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) of the ribosomal RNA internal transcribed spacer region (ITS) with restriction DdeI enzyme, were able to separate molecularly the molluscs genus Biomphalaria, such as B. glabrata, B. tenagophila, and B. straminea, which are intermediate hosts of S. mansoni in Brazil. Those authors obtained species-specific profiles for this three species, observing besides that various Brazilian populations of B. tenagophila showed a characteristic profile, with two fragments (800 and 470 pb). Barbosa (2001), using the same technique, observed that the Taim population, in addition to the characteristic species-specific profile of B. tenagophila, as described by Vidigal et al. (1998), has a 350 pb fragment too.In the present study, cross-breedings between molluscs from B. tenagophila (Taim) and B. tenagophila (Joinville) were carried out, aiming at verifying whether the marker of the Taim population has a dominant character.Studies in order to obtain F1 generation were undertaken using a specimen of B. tenagophila Taim and another one of albino B. tenagophila Joinville. They were maintained together in order to achieve cross-breeding (4 pairs). The albino recessive character was used, in this case, as a phenotypic marker. The molluscs with 5 mm in diameter, approximately, sexually immature, were put together into a plastic recipient with 100 ml dechlorinated water for 50 days. Afterwards, the respective pairs were separately put into glasses in order to obtain F1 descendants. In this experiment, the egg-layings from the albino snails, as well as from the pigmented ones, were collected for four weeks. The egg-layings from F1 generation were transferred to an aquarium until the molluscs could reach 5 mm in diameter. F2 generation was obtained by means of cross-breedings between F1 individuals according to the same procedures previously described.A fragment from the cephalopodal region of the molluscs was col...

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“…The ITS2 region was amplified using the forward (F) primer ITS2F (5'-CGTCCGTCTGAGGGTCGGTTTGC-3') anchored in the 5.8 region (Vidigal et al 2000) and the reverse (R) primer ETTS1 (5'-TGCTTAAGTTCAGCGGGT-3') anchored in the 28S region (Kane & Rollinson 1994). The primer ITS1R was designed based upon analysis of 5.8s sequences as described by Vidigal et al (2000b).…”

Section: Methodsmentioning

confidence: 99%

“…PCR amplification -The ITS1 region was amplified using the primers ETTS2 (5'-TAACAAGGTTT CCGTAGGTGAA-3 ' ) (Kane & Rollinson 1994) and ITS1R (5'-ACGAGCGAGTGATCCACCGC-3') anchored in the 18S and 5.8S regions of the rDNA, respectively. The ITS2 region was amplified using the forward (F) primer ITS2F (5'-CGTCCGTCTGAGGGTCGGTTTGC-3') anchored in the 5.8 region (Vidigal et al 2000) and the reverse (R) primer ETTS1 (5'-TGCTTAAGTTCAGCGGGT-3') anchored in the 28S region (Kane & Rollinson 1994).…”

Section: Methodsmentioning

confidence: 99%

Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae)

Vidigal

Spatz

Kissinger

et al. 2004

Mem. Inst. Oswaldo Cruz

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Repetitive sequences in the ribosomal DNA internal transcribed spacer of Schistosoma haematobium, Schistosoma intercalatum and Schistosoma mattheei

Cited by 140 publications

References 20 publications

Use of ITS rDNA for discriminating of larval stages of two microphallid (Digenea) species using Hydrobia ulvae (Pennant, 1777) and Corophium volutator (Pallas, 1766) as intermediate hosts

Use of ITS rDNA for discriminating of larval stages of two microphallid (Digenea) species using Hydrobia ulvae (Pennant, 1777) and Corophium volutator (Pallas, 1766) as intermediate hosts

Dominant character of the molecular marker of a Biomphalaria tenagophila strain (Mollusca: Planorbidae) resistant to Schistosoma mansoni

Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae)

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