Repetitive somatic embryogenesis and plant regeneration in Zizyphus jujuba Mill

Kim, Y. W.; Moon, Heung-Kyu; Son, Sung-Won

doi:10.1079/ivp2006754

Cited by 10 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, germination of the primary somatic embryos was negatively affected, because the intense production of secondary embryos on the surface of the primary ones impaired their conversion into plantlets by inhibiting root and aerial part development. Secondary embryogenesis was also reported for Quercus suber (Puigderrajols et al 1996), Arachis hypogaea (Little et al 2000), Bixa orellana (Paiva Neto et al 2003), Castanea sativa (Corredoira et al 2003), Acacia Arabica (Nanda and Rout 2006), Garcinia indica (Thengane et al 2006), Zizyphus jujuba (Kim et al 2006) and Dianthus caryophillus (Karami et al 2008).…”

Section: Resultsmentioning

confidence: 66%

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

Silva

Pinto

Guerra

et al. 2009

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The objective of the present work was to induce somatic embryogenesis from zygotic embryos of Passiflora cincinnata Masters. Zygotic embryos formed calli on media with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.5 lM benzyladenine (BA) after 30 days of in vitro culture. A concentration of 18.1 lM 2,4-D resulted in the largest number of somatic embryos. Embryogenic calli were yellowish and friable, forming whitish proembryogenic masses. Morphologically, embryogenic cells were small and had large nuclei and dense cytoplasm, whereas non-embryogenic cells were elongated, with small nuclei and less dense cytoplasm. Calli cultured under white light on basal Murashige and Skoog's medium with activated charcoal produced embryos in all developmental stages. There were differences among the treatments, with some leading to the production of calli with embryos and some only to callus formation. Some abnormalities were associated with somatic embryos, including fused axes, fused cotyledons and polycotyledonary embryos. Production of secondary somatic embryos occurred in the first cycle of primary embryo development. Secondary embryos differentiated from the surface of the protodermal layer of primary embryos with intense cell proliferation, successive mitotic divisions in the initial phase of embryoid development, and a vascular system formed with no connection to the parental tissue. This secondary embryogenic system of P. cincinnata is characterized by intense proliferation and maintenance of embryogenic competence after successive subcultures. This reproducible protocol opens new prospects for massive propagation and is an alternative to the current organogenesis-based transformation protocol.

show abstract

Section: Resultsmentioning

confidence: 66%

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

Silva

Pinto

Guerra

et al. 2009

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…The tissue culture of Z. jujuba Mill. was successfully obtained for several distinguished cultivars via leaf (Li et al, 2002;Gu et al, 2005;Chen et al, 2008;Feng et al, 2010;Ma et al, 2012;Ye et al, 2012), embryos Qi, 2003, Kim et al, 2006), cotyledons (Kim et al, 2006) and nodal ex-plants (Goyal et al, 2006;Soliman and Hegazi, 2013). However, the response of the planting material on tissue culture media depends on the genotype and therefore, it is important to develop a proper in-vitro protocol for Jujube varieties adapted to Sri Lankan condition to promote the commercial scale cultivation.…”

Section: Shoot Multiplicationmentioning

confidence: 99%

Micropropagation of <i>Ziziphus jujube</i> Mill. (Jujube) through shoot tip and nodal segment culture

Hansika¹,

Ranawake²,

Perera³

2017

Trop Agric Res & Ext

View full text Add to dashboard Cite

Jujube is known as Masan in Sri Lanka is one of the underutilized fruit crops. It is important due to its high nutritious value and introduced as a potential crop for commercial cultivation. Micropropagation using plant tissue culture is an efficient method for vegetative propagation of commercially important crops for mass production to meet the demands of planting materials. Present study was carried out to develop a proper in-vitro protocol for local varieties of Jujube to produce planting materials. The most suitable fungicide for surface sterilization procedure was selected by testing Captan (Captan 50%, 1.2 g/l), Topsin (Thiophanate methyl 70%, 2 g/l), and Daconil (Chlorothalonil, 1.8 ml/l). Selection of a suitable concentration of BAP (Benzyle Amino Purine) or TDZ (Thidiazuron) for shoot proliferation was assessed in four different concentrations of BAP (1, 1.5, 2, 2.5 mg/l) and of TDZ (0.1, 0.2, 0.3, 0.4 mg/l) and IBA (Indole Butric Acid) for root induction in two concentrations of IBA (1, 2 mg/l). Dipping shoot tips in Captan solution for 20 minutes gave the highest significant non-contamination percentage (79.9%) and lowest fungal radius (0.15 cm) of contaminated cultures among three treatments. MS (Murrashige and Skoog) medium containing 1.5 mg/l BAP recorded significantly highest percentage of elongated bud (96.66%) and newly produced shoot length (1.08 cm) and lowest significant rate was recorded in TDZ 0.2 mg/l (3.33%). Callus was produced in all the concentrations of TDZ. None of the concentrations of BAP or TDZ produced multiple shoots. Elongated nodal segments in BAP (1, 1.5 mg/l) could be successfully sub-cultured for further multiplication. Rooting was not recorded in both shoot tips and in-vitro generated shoots during four weeks of culturing on IBA contained media.

show abstract

“…However, micropropagation is more suitable than other vegetative propagation methods for jujube . Several micropropagation techniques such as somatic embryogenesis (Kim et al 2006), organogenesis (Gu and Zhang 2005) and shoot tip culture (Sudhersan et al 2001;Danthu et al 2004) have been carried out for in vitro propagation of jujube. Among these methods, shoot tip culture is used for jujube clonal propagation (Sudhersan et al 2001;Sudhersan and Hussain 2003).…”

Section: Introductionmentioning

confidence: 99%