Replacement of acetonitrile by ethanol as solvent in reversed phase chromatography of biomolecules

Brettschneider, F.; Jankowski, Vera; Günthner, Thomas; Salem, Silvia; Nierhaus, Marc; Schulz, Anna; Zidek, Walter; Jankowski, Joachim

doi:10.1016/j.jchromb.2010.01.029

Cited by 36 publications

(14 citation statements)

References 13 publications

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“…Isolated uremic toxins were separated by gradient elution on a Chromolith Performance RP‐18e (100 × 4.6 mm I.D., Merck, Darmstadt, Germany) reversed‐phase column in the presence of tetrabutylammonium sulfate (4 mmol/L) in a phosphate buffer (20 mmol/L K 2 HPO 4 , final concentration; pH 6.5) as eluent A. Ethanol with 0.1% trifluoroacetic acid (pH 6.5) was used as eluent B .…”

Section: Methodsmentioning

confidence: 99%

Removal of Protein‐Bound, Hydrophobic Uremic Toxins by a Combined Fractionated Plasma Separation and Adsorption Technique

et al. 2013

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Protein-bound uremic toxins, such as phenylacetic acid, indoxyl sulfate, and p-cresyl sulfate, contribute substantially to the progression of chronic kidney disease (CKD) and cardiovascular disease (CVD). However, based on their protein binding, these hydrophobic uremic toxins are poorly cleared during conventional dialysis and thus accumulate in CKD-5D patients. Therefore, we investigated whether hydrophobic and cationic adsorbers are more effective for removal of protein-bound, hydrophobic uremic toxins than conventional high-flux hemodialyzer. Five CKD-5D patients were treated using the fractionated plasma separation, adsorption, and dialysis (FPAD) system for 5 h. A control group of five CKD patients was treated with conventional high-flux hemodialysis. Plasma concentrations of phenylacetic acid, indoxyl sulfate, and p-cresyl sulfate were measured. Removal rates of FPAD treatment in comparison to conventional high-flux hemodialysis were increased by 130% for phenylacetic acid, 187% for indoxyl sulfate, and 127% for p-cresol. FPAD treatment was tolerated well in terms of clinically relevant biochemical parameters. However, patients suffered from mild nausea 2 h after the start of the treatment, which persisted until the end of treatment. Due to the high impact of protein-bound, hydrophobic uremic toxins on progression of CKD and CVD in CKD-5D patients, the use of an adsorber in combination with dialysis membranes may be a new therapeutic option to increase the removal rate of these uremic toxins. However, larger, long-term prospective clinical trials are needed to demonstrate the impact on clinical outcome.

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Section: Methodsmentioning

confidence: 99%

Removal of Protein‐Bound, Hydrophobic Uremic Toxins by a Combined Fractionated Plasma Separation and Adsorption Technique

et al. 2013

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“…for its synthesis is acetonitrile. Acetonitrile is a major solvent because of its use in chromatography [ 236 ], and it is co-produced with acrylonitrile from propylene [ 237 ]. Unfortunately this means the availability and price of acetonitrile is strongly related to the acrylonitrile plastics market, and a shortage even occurred in 2008 [ 238 , 239 ].…”

Section: Propylenementioning

confidence: 99%

Opportunities for Bio-Based Solvents Created as Petrochemical and Fuel Products Transition towards Renewable Resources

Clark

Farmer

Hunt

et al. 2015

IJMS

208

101

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The global bio-based chemical market is growing in size and importance. Bio-based solvents such as glycerol and 2-methyltetrahydrofuran are often discussed as important introductions to the conventional repertoire of solvents. However adoption of new innovations by industry is typically slow. Therefore it might be anticipated that neoteric solvent systems (e.g., ionic liquids) will remain niche, while renewable routes to historically established solvents will continue to grow in importance. This review discusses bio-based solvents from the perspective of their production, identifying suitable feedstocks, platform molecules, and relevant product streams for the sustainable manufacturing of conventional solvents.

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“…While not undertaken in this study, the application of monolithic columns in both dimensions would allow the development of a rapid 2D‐HPLC separation for the production of individual ω3 LC‐PUFA esters (>95% purity) from fish oil esters. Furthermore, acetonitrile can be replaced with ethanol in the RP‐HPLC separation enabling rapid separation of ω3 LC‐PUFA esters on monolithic RP columns using ethanol/water elution gradients, which is potentially suitable for food processes.…”

Section: Discussionmentioning

confidence: 99%

Rapid isolation of omega-3 long-chain polyunsaturated fatty acids using monolithic high performance liquid chromatography columns

Fagan

Wijesundera

2013

J. Sep. Science

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Eicosapentaenoic and docosahexaenoic acids are important bio-active fatty acids in fish oils. Monolithic HPLC columns both in the polymeric cation exchange (silver-ion) and RP formats were compared with corresponding packed columns for the isolation of these acids from tuna oil ethyl esters. Monolithic columns in both formats enabled rapid (typically 5-10 min) separations compared with packed columns (30 min). Polymeric monolithic silver-ion disc column rapidly furnished mixtures of eicosapentaenoic and docosahexaenoic esters (90% purity) within 5-10 min, but was unable to resolve individual esters. A preparative version of the same column (80 mL bed volume) enabled isolation (>88% purity) of 100 mg quantities of eicosapentaenoic and docosahexaenoic esters from esterified tuna oil within 6 min. Baseline separation of eicosapentaenoic and docosahexaenoic esters was achieved on all RP columns. The results show that there is potential to use polymeric monolithic cation exchange columns for scaled-up preparation of eicosapentaenoic and docosahexaenoic ester concentrates from fish oils.

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Replacement of acetonitrile by ethanol as solvent in reversed phase chromatography of biomolecules

Cited by 36 publications

References 13 publications

Removal of Protein‐Bound, Hydrophobic Uremic Toxins by a Combined Fractionated Plasma Separation and Adsorption Technique

Removal of Protein‐Bound, Hydrophobic Uremic Toxins by a Combined Fractionated Plasma Separation and Adsorption Technique

Opportunities for Bio-Based Solvents Created as Petrochemical and Fuel Products Transition towards Renewable Resources

Rapid isolation of omega-3 long-chain polyunsaturated fatty acids using monolithic high performance liquid chromatography columns

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