Replacement of Large Veins with Free Inverted Segments of Small Bowel

Matsumoto, Teruo; Holmes, Robert H.; Burdick, Claude O.; Heisterkamp, Charles A.; O'Connell, Thomas J.

doi:10.1097/00000658-196611000-00009

Cited by 23 publications

(4 citation statements)

References 6 publications

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“…SIS was first used more than 50 years ago when it was used as an autologous alternative to the superior and inferior vena cava in dogs [ 18 ]. This paved the way for using SIS as a biological scaffold to regenerate tissue.…”

Section: Introductionmentioning

confidence: 99%

The application of small intestinal submucosa in tissue regeneration

Zhao,

Peng,

Sun

et al. 2024

Materials Today Bio

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Section: Introductionmentioning

confidence: 99%

The application of small intestinal submucosa in tissue regeneration

Zhao,

Peng,

Sun

et al. 2024

Materials Today Bio

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“…Two examples of ECM scafolds for wound management are small intestinal submucosa (SIS) and ovine forestomach matrix (OFM). SIS was frst used in the 1960s as a vascular substitute [13]. Since then, the utility of SIS has been demonstrated in various reconstructive therapies including wound repair, hernia repair, colon/rectal surgery, bladder repairs, and musculoskeletal reconstruction [14].…”

Section: Introductionmentioning

confidence: 99%

Varying Properties of Extracellular Matrix Grafts Impact Their Durability and Cell Attachment and Proliferation in an In Vitro Chronic Wound Model

Harmon,

Burnette,

Avery

et al. 2024

Journal of Tissue Engineering and Regenerative Medicine

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While acute wounds typically progress through the phases of wound healing, chronic wounds often stall in the inflammatory phase due to elevated levels of matrix metalloproteinases (MMPs) and proinflammatory cytokines. Dysregulated expression of MMPs can result in the breakdown of extracellular matrix (ECM) formed during the wound healing process, resulting in stalled wounds. Native collagen-based wound dressings offer a potential wound management option to sequester excess MMPs and support cellular interactions that allow wound progression through the natural healing process. Herein, we utilized commercially available ECM matrices, two derived from porcine small intestinal submucosa (PCMP, 2 layers; PCMP-XT, 5 layers) and one derived from propria submucosa (ovine forestomach matrix, OFM, 1 layer), to demonstrate the impact of processing methodologies (e.g., layering and crosslinking) on functional characteristics needed for the management of chronic wounds. Grafts were evaluated for structural composition using scanning electron microscopy and histology, ability to reduce MMPs using fluorometric assays, and durability in an in vitro degradation chronic wound model. Both intact (nondegraded) and partially degraded grafts were assessed for their ability to serve as a functional cell scaffold using primary human fibroblasts. Grafts differed in matrix substructure and composition. While all grafts demonstrated attenuation of MMP activity, PCMP and PCMP-XT showed larger reductions of MMP levels. OFM rapidly degraded in the in vitro degradation model (<3 hours), while PCMP and PCMP-XT were significantly more durable (>7 days). The ability of PCMP and PCMP-XT to serve as scaffolds for cellular attachment was not impacted by degradation in vitro. Three ECM grafts with varying structural and functional characteristics exhibited differential durability when degraded in a simulated chronic wound model. Those that withstood rapid degradation maintained their ability to function as a scaffold to support attachment and proliferation of fibroblasts, a cell type important for wound healing.

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“…Porcine-derived SIS from small intestinal tissue applied as a completely decellularized matrix was first described as a vascular substitute. [ 15 , 16 ] Research has shown that decellularized SIS contains bioactive factors such as fibronectin, glycosaminoglycan (GAG), and growth factors, [ 17 ] as well as bioactive factors involved in cell adhesion, mitogenesis, and chemotaxis. [ 18 ] Ligament reconstruction using SIS was demonstrated to be effective in equine, caprine, and canine models.…”

Section: Introductionmentioning

confidence: 99%

An injectable nucleus pulposus cell-modified decellularized scaffold: biocompatible material for prevention of disc degeneration

et al. 2017

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We developed a nucleus pulposus cell (NPC)-modulated decellularized small intestinal submucosa (SIS) scaffold, and assessed the ability of this material to prevent Intervertebral disc degeneration (IVD) degeneration. Decellularized porcine SIS was squashed into particles and the biological safety and efficiency of these particles were evaluated. Next, SIS particles were seeded with rabbit NPCs, cultured for two months in vitro, decellularized again and suspended for intervertebral injection. We demonstrated that use of the decellularization protocol resulted in the removal of cellular components with maximal retention of extracellular matrix. The xenogeneic decellularized SIS did not display cytotoxicity in vitro and its application prevented NPC degradation. Furthermore, the xenogeneic SIS microparticles were effective in preventing IVD progression in vivo in a rabbit disc degeneration model. In conclusion, our study describes an optimized method for decellularized SIS preparation and demonstrated that the material is safe and effective for treating IVD degeneration.

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Replacement of Large Veins with Free Inverted Segments of Small Bowel

Cited by 23 publications

References 6 publications

The application of small intestinal submucosa in tissue regeneration

The application of small intestinal submucosa in tissue regeneration

Varying Properties of Extracellular Matrix Grafts Impact Their Durability and Cell Attachment and Proliferation in an In Vitro Chronic Wound Model

An injectable nucleus pulposus cell-modified decellularized scaffold: biocompatible material for prevention of disc degeneration

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