Replacement of the deletion in the genome (0.762–0.789 mu) of avirulent HSV-1 HFEM using cloned Mlul DNA fragment (0.7615–0.796 mu) of virulent HSV-1 F leads to generation of virulent intratypic recombinant

The Herpes Simplex Virus Type 2 (HG52) Variant JH2604 Has a 1488 bp Deletion which Eliminates Neurovirulence in Mice

“…To this end specific viral genes and regions of the HSV genome as well as many host factors have been implicated in the control of virulence (Kohl & Loo, 1980;Caspary et al, 1980;Field & Wildy, 1978;Thompson & Stevens, 1983;Thompson et al, 1985Thompson et al, , 1986Javier et al, 1986;Rosen et al, 1986;Field & Coen, 1986). Moreover heterogeneity of virulence within a single virus stock of HSV-2 strain HG52 has recently been demonstrated.…”

A Variant of Herpes Simplex Virus Type 2 Strain HG52 with a 1.5 kb Deletion in RL between 0 to 0.02 and 0.81 to 0.83 Map Units Is Non-neurovirulent for Mice

“…Viral genes controlling neurovirulence were only identified recently, and various laboratories have predominantly implicated the viral sequences between 0.7 to 0-83 m.u. in the control of virulence (Thompson & Stevens, 1983;Thompson et al, 1985;Javier et al, 1987;Rosen et al, 1986). This region contains the whole of IRL and part of UL.…”

“…(Rosen & Darai, 1985) has been shown to be non-pathogenic for tree shrews and mice, and its pathogenicity can be restored by the insertion of sequences spanning 0.76 to 0.79 m.u. from the pathogenic strain F (Rosen et al, 1986).…”

“…have been shown to be involved in determining the virulence phenotype (Thompson & Stevens, 1983;Thompson et al, 1985 ;Javier et al, 1986;Rosen et al, 1986). Sequences between 0.25 and 0-53 m.u.…”

Alphaherpesvirus Saimiri in Rabbits: A Model For Human Encephalitis?

“…There seems to be a factor in the makeup of strain KM322 which restrains its inherent rabbit neurovirulence and which can also restrain co-inoculated neuroinvasive KM91. Although short specific HSV DNA sequences have recently been shown to control neurovirulence in mice and tree shrews (Rosen et al, 1986) the nature and function of the gene(s) involved are still unknown.…”