2024
DOI: 10.1101/2024.01.31.577620
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Replicating enzymatic activity by positioning active sites with synthetic protein scaffolds

Yujing Ding,
Shanshan Zhang,
Henry Hess
et al.

Abstract: Evolutionary constraints significantly limit the diversity of naturally occurring enzymes, thereby reducing the sequence repertoire available for enzyme discovery and engineering. Recent breakthroughs in protein structure prediction and de novo design, powered by artificial intelligence, now enable us to create enzymes with desired functions without relying on traditional genome mining. Here, we demonstrate a computational strategy for creating new-to-nature PET hydrolases by leveraging the known catalytic mec… Show more

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