Replicating Marek's disease virus (MDV) serotype 2 DNA with inserted MDV serotype 1 DNA sequences in a Marek's disease lymphoblastoid cell line MSB1-41C

Abstract

We characterized the properties of herpes-type viruses which grew well in a Marek's disease lymphoblastoid cell line, MSB1-41C, inducing cytopathic effect characterized by the formation of syncytial giant cells. Examination of the infectious virus by field inversion gel electrophoresis revealed the presence of DNA of about 180 kbp in both the culture fluid and cell fractions of the infected MSB-41C cells. The DNA was found to consist of Marek's disease virus (MDV) serotype 2 (MDV2) and MDV serotype 1 (MDV1) DNA by Southern blot hybridization. The MDV1 DNA consisted of sequences mainly from the long inverted repeats including multiple copies of 132 bp direct tandem repeats. Molecular cloning of BamHI digests of the MDV2 DNA revealed a fragment of MDV1 DNA and MDV2 DNA fused together, indicating that the recombinant MDV2 DNA had been generated by genetic recombination with the latent MDV1 DNA.

MicroRNAs 221 and 222 target p27Kip1 in Marek's disease virus-transformed tumour cell line MSB-1

“…However, it is possible that certain dissimilarities of MSB-1 cells compared to the T226S and T265L cells might be linked to the observed variation in miR-221/miR-222 expression. These include the co-infection of MSB-1 with BC-1 strain of MDV-1 and HPRS-24 strain of MDV-2 (Hirai et al, 1990;Yao et al, 2007), interference with the p53 pathways due to truncated p53 transcripts (Takagi et al, 2006a, b), differences in the methylation status (Kanamori et al, 1987), and that the RB-1B strain used to transform the other cell lines is more lymphomagenic than BC-1. Also of particular interest was the observed downregulation of p27 Kip1 in all MDV-tumour cell lines despite differential miR-221/miR-222 expression.…”

“…+ T-cell line derived from a spleen lymphoma induced by the BC-1 strain of MDV-1, (Akiyama & Kato, 1974;Hirai et al, 1990) is capable of inducing tumours when inoculated into susceptible chickens (Doi et al, 1976;Lee et al, 1975). We have recently shown that MSB-1 expresses high levels of MDV-1-and MDV-2-encoded miRNAs Yao et al, 2007Yao et al, , 2008, many of which are expressed at much higher levels than those in infected chicken embryo fibroblasts (CEF) (Burnside et al, 2006;Burnside & Morgan, 2007;Burnside et al, 2008).…”

A p53-dependent promoter associated with polymorphic tandem repeats controls the expression of a viral transcript encoding clustered microRNAs

“…The MSB-1 cell line is derived from a spleen lymphoma induced by a virulent strain of MDV-1 (Akiyama et al 1973). It was previously shown to be coinfected with both MDV-1 (BC-1) and MDV-2 (HPRS24) (Hirai et al 1990). The 54-O cell line was isolated in the TLVI laboratory (INRA, Nouzilly) from an ovary lymphoma induced by recombinant RB1-B virus reconstituted from an infectious genome cloned as a bacterial artificial chromosome (kindly provided by Venugopal Nair).…”

MicroRNA Profile of Marek's Disease Virus-Transformed T-Cell Line MSB-1: Predominance of Virus-Encoded MicroRNAs

“…MSB-1 is an MDV-transformed CD4 ϩ T-cell line derived from a spleen lymphoma induced by the BC-1 strain of MDV-1 (1,30). The MSB-1 cell line, used in this study at passage level 13, has a CD4 ϩ phenotype and has been shown to be coinfected with MDV-1 and MDV-2 (66).…”