Replication and Compression of Surface Structures with Polydimethylsiloxane Elastomer

Lisensky, George C.; Campbell, Dean J.; Beckman, Katie J.; Calderón, Camilo E.; Doolan, Patrick W.; Ottosen, Rebecca M.; Ellis, Arthur B.

doi:10.1021/ed076p537

Cited by 76 publications

(34 citation statements)

References 15 publications

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“…As the ratio of the curing agent to resin increases, a more rigid elastomer is produced. 27 Another effect could be that the elastomer plane, particularly at the fabricated face, has an inaccurate flat surface.…”

Section: Resultsmentioning

confidence: 99%

Highly Flexible, Stretchable, and Tunable Optical Diffusers with Mechanically Switchable Wettability Surfaces

Alqurashi

Butt

2019

ACS Cent. Sci.

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Highly stretchable and super-hydrophobic photonics provides a new geometric degree of freedom for photonic system design and self-cleaning applications. Here, we describe the design and experimental realization of mechanically stretchable and tunable photonic diffusers. These intrinsically designed diffusers (based on periodic arrays of cylindrical lenslets and microtip) were made directly on elastomer material using laser ablation. The dimensions of both the tips and the lenslet arrays play a critical role in the distribution of illumination and wettability resistance. By stretching the diffusers mechanically along the lenslet arrays, diffusion angle tuning was achieved and also a reversible change between hydrophilic to super-hydrophobic states. These multifunctional diffusers constitute an important step toward integration with flexible materials or devices such as stretchable organic light-emitting diodes and polymer light-emitting diodes.

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Section: Resultsmentioning

confidence: 99%

Highly Flexible, Stretchable, and Tunable Optical Diffusers with Mechanically Switchable Wettability Surfaces

Alqurashi

Butt

2019

ACS Cent. Sci.

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“…The device was cleaned with ethanol and activated together with a 24 × 60 mm coverslip (#1, Carl Roth, Germany) in an oxygen plasma (PVA TePla 100, PVA TePla, Germany; 0.45 mbar, 200 W, 20 s). 24 After activation, the PDMS device was pressed on the coverslip and heated for at least 2 h at 65 °C. Sigmacote (Sigma-Aldrich, Germany) was applied to the microchannels of the device in order to make their surfaces hydrophobic.…”

Section: Methodsmentioning

confidence: 99%

Reconceptualizing Fluorescence Correlation Spectroscopy for Monitoring and Analyzing Periodically Passing Objects

et al. 2017

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Fluorescence correlation spectroscopy (FCS) is a sensitive technique commonly applied for studying the dynamics of nanoscale-labeled objects in solution. Current analysis of FCS data is largely based on the assumption that the labeled objects are stochastically displaced due to Brownian motion. However, this assumption is often invalid for microscale objects, since the motion of these objects is dominated by Stokes drag and settling or rising effects, rather than stochastic Brownian motion. To utilize the power of FCS for systems with nonstochastic displacements of objects, the collection and analysis of FCS data have to be reconceptualized. Here, we extended the applicability of FCS for the detection and analysis of periodically passing objects. Toward this end, we implemented droplet-based microfluidics, in which monodispersed droplets containing fluorescent marker are flowing equally spaced within microchannels. We show by simulations and experiments that FCS can sensitively quantify the flow-rates, variability, and content of rapidly passing droplets. This information can be derived at high temporal resolution, based on the intensity fluctuations generated by only 5–10 passing droplets. Moreover, by utilizing the periodicity of the flowing droplets for noise reduction by averaging, FCS can monitor accurately the droplets flow even if their fluorescence intensity is negligible. Hence, extending FCS for periodically passing objects converts it into a powerful analytical tool for high-throughput droplet-based microfluidics. Moreover, based on the principles described here, FCS can be straightforwardly applied for a variety of systems in which the passing of objects is periodic rather than stochastic.

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“…EcoFAB is a sterile plant growth platform that includes two primary components: the EcoFAB device and sterile plant-sized transparent container. The EcoFAB device is made from a polydimethylsiloxane (PDMS) manufacturing process that involves casting PDMS layers from a 3D printed plastic mold and bonding PDMS layers onto microscope slides using methods previously reported 24 25 . The detailed procedures of EcoFAB workflow, such as device fabrication, sterilization, seed germination, seedling transplantation, microbe inoculation/cocultivation, sample preparation, and analysis, are described in this protocol ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

Gao

Sasse

Lewald

et al. 2018

JoVE

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Beneficial plant-microbe interactions offer a sustainable biological solution with the potential to boost low-input food and bioenergy production. A better mechanistic understanding of these complex plant-microbe interactions will be crucial to improving plant production as well as performing basic ecological studies investigating plant-soil-microbe interactions. Here, a detailed description for ecosystem fabrication is presented, using widely available 3D printing technologies, to create controlled laboratory habitats (EcoFABs) for mechanistic studies of plant-microbe interactions within specific environmental conditions. Two sizes of EcoFABs are described that are suited for the investigation of microbial interactions with various plant species, including Arabidopsis thaliana, Brachypodium distachyon, and Panicum virgatum. These flow-through devices allow for controlled manipulation and sampling of root microbiomes, root chemistry as well as imaging of root morphology and microbial localization. This protocol includes the details for maintaining sterile conditions inside EcoFABs and mounting independent LED light systems onto EcoFABs. Detailed methods for addition of different forms of media, including soils, sand, and liquid growth media coupled to the characterization of these systems using imaging and metabolomics are described. Together, these systems enable dynamic and detailed investigation of plant and plant-microbial consortia including the manipulation of microbiome composition (including mutants), the monitoring of plant growth, root morphology, exudate composition, and microbial localization under controlled environmental conditions. We anticipate that these detailed protocols will serve as an important starting point for other researchers, ideally helping create standardized experimental systems for investigating plant-microbe interactions.

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Replication and Compression of Surface Structures with Polydimethylsiloxane Elastomer

Cited by 76 publications

References 15 publications

Highly Flexible, Stretchable, and Tunable Optical Diffusers with Mechanically Switchable Wettability Surfaces

Highly Flexible, Stretchable, and Tunable Optical Diffusers with Mechanically Switchable Wettability Surfaces

Reconceptualizing Fluorescence Correlation Spectroscopy for Monitoring and Analyzing Periodically Passing Objects

Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

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