Replication of Cytopathic and Noncytopathic Bovine Viral Diarrhea Virus in Zona- Free and Zona-lntact In Vitro-Produced Bovine Embryos and the Effect on Embryo Quality1

Vanroose, G.; Nauwynck, Hans; Soom, Ann Van; Vanopdenbosch, E.; Kruif, Aart de

doi:10.1095/biolreprod58.3.857

Cited by 56 publications

(31 citation statements)

References 45 publications

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“…It might therefore be possible to conclude that the stage from the mature oocyte to the 2-cell embryo or 4-cell embryo stage is not susceptible to NCP BVDV. The virus titer of hatched blastocysts was low, the same as in the results reported by Vanroose et al [10], but in our study the virus could be isolated from two samples at 24 hr, findings that differed from theirs, and we clearly demonstrated the presence of NCP BVDV specific antigen in hatched blastocysts ( Fig. 1.-b).…”

supporting

confidence: 90%

“…These findings suggest that the 2-and 4-cell embryos are not susceptible to NCP BVDV and that they acquire susceptibility to the virus when they develop into the hatched blastocysts. Vanroose et al [10] recently reported that mature oocytes and fertilized ova could not be infected with NCP BVDV. It might therefore be possible to conclude that the stage from the mature oocyte to the 2-cell embryo or 4-cell embryo stage is not susceptible to NCP BVDV.…”

mentioning

confidence: 99%

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Susceptibility of Bovine Naked 2- and 4-Cell Embryos and Hatched Blastocysts Produced In Vitro to Infection with Noncytopathogenic Bovine Viral Diarrhea Virus.

Tbuboi

Imada

1999

The Journal of Veterinary Medical Science

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ABSTRACT. To investigate the susceptibility of early bovine embryos to noncytopathogenic bovine viral diarrhea virus (NCP BVDV), 2-and 4-cell embryos produced in vitro from which zona pellucida had been removed by pronase treatment, and hatched blastocysts were exposed to 10 6 TCID 50 /ml of NCP BVDV No. 12 strain. The virus was detected in all embryo samples immediately prior to cultivation but not in the medium. After 24-hr culture, the virus was isolated from four media and two embryo samples in four experiments in the blastocyst group, and the viral antigen was demonstrated in the cytoplasm of the embryo cells by the immunofluorescent technique. By contrast, no virus was recovered from, or viral antigen detected in samples from the 2-and 4-cell embryo group in any of the experiments, even though they were exposed to the virus after removal of the zona pellucida. These findings suggest that 2-and 4-cell embryos are unlikely to be susceptible to NCP BVDV, but that blastocysts are capable of being infected with the virus.-KEY WORDS: bovine embryo, hatched blastocyst, noncytopathogenic bovine viral diarrhea virus.J. Vet. Med. Sci. 61(8): 943-945, 1999 culture. The blastocysts that hatched naturally from the ZP were collected, and used. These naked embryos were exposed to NCP BVDV No. 12 strain, 10 6 TCID50/ml. After 1 hr of culture at 37°C under 5% CO2, the embryos were washed by transferring them through six changes of wash medium. The washed naked embryos were cultured for 24 hr in a 0.1-ml drop covered with mineral oil (E. R. Squibb & Sons, Inc., U.S.A.) in a small Petri dish (35 × 10 mm: Nunc, Denmark). Eighty 2-and 4-cell embryos were transferred to the development medium. Forty embryos were immediately collected as 0-hr 2-and 4-cell embryos, and development medium also was collected as 0-hr 2-and 4-cell medium. Using the same method, eight hatched blastocysts were transferred to the development medium and four blastocysts were immediately collected as 0-hr hatched blastocysts, and the development medium was collected as 0-hr hatched blastocyst medium. Fresh development medium was added to culture the remaining 2-and 4-cell embryos and hatched blastocysts. After 24 hr of culture, the media were collected, and the embryos were washed by transferring them through three changes of wash medium, then suspended in 1 ml of minimum essential medium supplemented with 0.2% bovine serum albumin and antibiotics, and sonicated for 30 sec. Each control group unexposed to virus also was prepared for observation of 2-and 4-cell embryos and hatched blastocyst development, and virus isolation from these embryos. The titers of NCP BVDV in the medium and embryo samples were determined by a micro-interference test using bovine fetal muscular (BFM) cells and the cytopathogenic Nose strain [6]. The virus titers were calculated and expressed as TCID50/0.1 ml and TCID50/all embryos for medium and embryo samples, respectively. The BFM cells and FBS used were confirmed to be free of BVDV. The experiments were repeated four times.F...

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supporting

confidence: 90%

mentioning

confidence: 99%

Susceptibility of Bovine Naked 2- and 4-Cell Embryos and Hatched Blastocysts Produced In Vitro to Infection with Noncytopathogenic Bovine Viral Diarrhea Virus.

Tbuboi

Imada

1999

The Journal of Veterinary Medical Science

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“…Reportedly, morula and blastocyst, hatched and freed in the pellucid zone, are more susceptible to bovine herpesvirus-1 (IBR) (Vanroose et al 1997, Graham 2013, and bovine viral diarrhea BVD virus (Vanroose et al 1998, Lanyon et al 2014. Therefore, in this study, the presence of an infectious agent as a cause for some of the losses can be inferred.…”

Section: Discussionmentioning

confidence: 90%

Immune-serological identification of infectious agents with influence on bovine embryo transfer in the north of Brazil

et al. 2016

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ABSTRACT. The aim of this study was to investigate antibodies against brucellosis, leptospirosis, bovine rhinotracheitis (IBR/ BoHV-1), bovine viral diarrhea (BVD) and neosporosis through immunodiagnostic. These agents may directly or indirectly compromise the female bovine reproductive tract as well as embryonic and fetal development, therefore we also tried to establish a probable relation between those antibody with the utilization rates of fixed-time embryo transfer (FTET) protocols, with pregnancy rate per embryo transfer (ET) and the occurrence of miscarriages. Embryos were obtained by in vitro fertilization of the ovum and sperm from the Gyr dairy breed, which were transferred to 235 zebu-crossbred cows under FTET protocols. The blood samples to obtain serum were collected by venous puncture from coccygeal vein, only a few days of the completion of ET. For the immunodiagnostic research of brucellosis, leptospirosis, IBR/BoHV-1, BVD and neosporosis, the complement fixation assay, the microscopic agglutination test, virus neutralization, and linked immunosorbent assay to enzymes in conjunction with the indirect immunofluorescence were used. The utilization rates of the FTET protocols related to pregnancy and abortion in D16, D35, and D75 were 67.23%, 34,18% and 20,37% respectively. The immunodiagnostic results related to seropositive animals were obtained for brucellosis (7/235 = 2.89%), leptospirosis (128/235 = 54.47%), IBR (103/235 = 43.83%), BVD (174/235 = 74.04%), and neosporosis (150/235 = 63.82%). In seropositive recipients, there was an observed influence (P < 0.05) of leptospirosis and neosporosis on the rate of protocol utilization of brucellosis on both the pregnancy and abortion rate, and of BVD and IBR on abortion rate.Key words: reproductive, embryo, recipients, cattle. RESUMEN.El estudio tuvo por finalidad evidenciar, mediante técnicas de inmunodiagnóstico, anticuerpos contra Brucelosis, Leptospirosis, Rinotraqueítis Infecciosa Bovina (IBR), Diarrea Viral Bovina (DVB) y Neosporosis, agentes que directa o indirectamente pueden comprometer el tracto reproductor de las hembras así como el desarrollo embrionario y fetal. Se estableció una probable relación entre la presencia de anticuerpos contra los agentes con la tasa de aprovechamiento de protocolos de transferencias de embriones en tiempo fijo (TETF), la tasa de preñez por transferencia de embriones (TE) y la ocurrencia de abortos. Los embriones fueron obtenidos por fertilización in vitro (FIV) a partir de aspiración folicular y semen de toros, ambos de raza Gyr lechero, y tranferidos a 235 vacas receptoras cruzadas con Cebú sometidas a protocolos para TETF. Se obtuvo suero de muestras de sangre mediante venopunción de la vena coccígea solamente en los días de realización de TE. Para evidenciar Brucelosis, Leptospirosis, IBR, DVB y Neospora se emplearon las técnicas de fijación de complemento, seroaglutinación microscópica, seroneutralización y test de inmunoensayo (ELISA) junto con la inmunofluorescencia indirecta (RIFI), respectivamente. El ...

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“…This inhibitory effect was not due to virus replication in the embryonic cells, since an intact zona pellucida acts as a protective barrier against BoHV-1 ( Vanroose et al 1997Vanroose et al , 1998. Therefore, other reasons need to be established to explain the adverse effect of BoHV-1 on embryonic development.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of bovine sperm–zona binding by bovine herpesvirus-1

Tanghe¹,

Vanroose²,

Soom³

et al. 2005

Reproduction

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The purpose of the present study was to identify a potential interference of bovine herpesvirus-1 (BoHV-1) with sperm-oocyte interactions during bovine in vitro fertilization. An inhibition of almost 70% of sperm -zona binding was observed when bovine cumulus-denuded oocytes were inseminated in the presence of 10 7 50% tissue culture infective dose/ml BoHV-1. The inhibitory effect of BoHV-1 on sperm -zona binding was mediated by an interaction of the virus with spermatozoa, but not with oocytes. Treatment of spermatozoa with BoHV-1, however, did not affect sperm motility and acrosomal status. Antiserum against BoHV-1 prevented the virus-induced inhibition of sperm -zona binding, indicating that BoHV-1 itself affects the fertilization process. In order to investigate which BoHV-1 glycoprotein(s) are responsible for the virus -sperm interaction, BoHV-1 was treated with monoclonal antibodies against the viral glycoproteins gB, gC, gD and gH prior to insemination. Anti-gC completely prevented the inhibitory effect of BoHV-1 on sperm-zona binding, while anti-gD caused a reduction of this inhibition. Further evidence for the involvement of gC and gD in the virus-sperm interaction was provided by the fact that purified gC and gD decreased sperm -zona binding in a dose-dependent way with gC being more effective than gD. These results indicated that BoHV-1 inhibits bovine sperm -zona binding by interacting with spermatozoa. The binding of BoHV-1 to a spermatozoon is mediated by the viral glycoproteins gC and gD, and therefore seems to be comparable with the mechanisms of BoHV-1 attachment to its natural host cell.Reproduction (

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Replication of Cytopathic and Noncytopathic Bovine Viral Diarrhea Virus in Zona- Free and Zona-lntact In Vitro-Produced Bovine Embryos and the Effect on Embryo Quality1

Cited by 56 publications

References 45 publications

Susceptibility of Bovine Naked 2- and 4-Cell Embryos and Hatched Blastocysts Produced In Vitro to Infection with Noncytopathogenic Bovine Viral Diarrhea Virus.

Susceptibility of Bovine Naked 2- and 4-Cell Embryos and Hatched Blastocysts Produced In Vitro to Infection with Noncytopathogenic Bovine Viral Diarrhea Virus.

Immune-serological identification of infectious agents with influence on bovine embryo transfer in the north of Brazil

Inhibition of bovine sperm–zona binding by bovine herpesvirus-1

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