Replication of decapod iridescent virus 1 in primary cultured hematopoietic tissue cells of Cherax quadricarinatus

You, Yanlin; Yang, Feng; Li, Fang

doi:10.1016/j.aquaculture.2022.739069

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…The significant new viruses decapod iridescent virus 1 (DIV1) and white spot syndrome virus (WSSV), which are detrimental to shrimp aquaculture, have greatly harmed and seriously damaged the shrimp farming business in China and around the world. , There are no specific medications available as of now. So, one of the most important steps in preventing the propagation and outbreak of the virus is quick and precise surveillance at the shrimp breeding location. , DIV1 and WSSV can be identified by their characteristic markers, the ATPase gene of DIV1 (GenBank: KY681040.1) and the VP66 gene of WSSV (GenBank: MN481520.1). , Gene sequences were used to design crRNA-specific binding sites and isothermal amplification primers (Figure S6).…”

Section: Resultsmentioning

confidence: 99%

Section: Diagnostic Bacterial Microrobotmentioning

confidence: 99%

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Living Magnetotactic Microrobots Based on Bacteria with a Surface-Displayed CRISPR/Cas12a System for Penaeus Viruses Detection

Chen,

Zhou,

et al. 2023

ACS Appl. Mater. Interfaces

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Bacterial microrobots are an emerging living material in the field of diagnostics. However, it is an important challenge to make bacterial microrobots with both controlled motility and specific functions. Herein, magnetically driven diagnostic bacterial microrobots are prepared by standardized and modular synthetic biology methods. To ensure mobility, the Mms6 protein is displayed on the surface of bacteria and is exploited for magnetic biomineralization. This gives the bacterial microrobot the ability to cruise flexibly and rapidly with a magnetization intensity up to about 18.65 emu g–1. To achieve the diagnostic function, the Cas12a protein is displayed on the bacterial surface and is used for aquatic pathogen nucleic acid detection. This allows the bacterial microrobot to achieve sensitive, rapid, and accurate on-site nucleic acid detection, with detection limits of 8 copies μL–1 for decapod iridescent virus 1 (DIV1) and 7 copies μL–1 for white spot syndrome virus (WSSV). In particular, the diagnostic results based on the bacterial microrobots remained consistent with the gold standard test results when tested on shrimp tissue. This approach is a flexible and customizable strategy for building bacterial microrobots, providing a reliable and versatile solution for the design of bacterial microrobots.

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