Replication of
            <i>Mycoplasma pneumoniae</i>
            in Broth Culture

Low, Iolanda E.; Eaton, Monroe D.

doi:10.1128/jb.89.3.725-728.1965

Cited by 34 publications

(16 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cell Growth When colony-forming units are assayed, mycoplasma cultures follow typical bacterial growth curves with exponential, stationary, and death phases (43,45,69,70,80,86,129,180). Mycoplasma colonies have been shown to form from single cells for M. gallisepticum (105) and M. arthritidis H39 (unpublished results of the present authors, using same experimental protocol as reference 105).…”

Section: Introductionmentioning

confidence: 67%

See 1 more Smart Citation

Cell biology of the mycoplasmas.

Maniloff¹,

Morowitz²

1972

Bacteriological Reviews

101

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 67%

“…The length of the stationary phase varies among the species (86,129) but is frequently shorter than 24 hr. The subsequent death rates are quite rapid; the titer drops by a factor of 102 to 104 per hr (80,86).…”

Section: Introductionmentioning

confidence: 99%

Cell biology of the mycoplasmas.

Maniloff¹,

Morowitz²

1972

Bacteriological Reviews

101

View full text Add to dashboard Cite

“…Organisms, media, and cultivation. Exponentialphase organisms of the FH and Mac strains of M. pneumoniae were grown in Hayflick medium, washed once, and concentrated as 10% cell suspensions as previously described (19,20).…”

Section: Methodsmentioning

confidence: 99%

Reduced Nicotinamide Adenine Dinucleotide Oxidase Activity and H ₂ O ₂ Formation of Mycoplasma pneumoniae

Low¹,

Zimkus

1973

J Bacteriol

Self Cite

View full text Add to dashboard Cite

Cell-free extracts of Mvcoplasma pneumoniae showed two distinct reduced nicotinamide adenine dinucleotide (NADH2) oxidase activities in the supernatant fraction. By ammonium sulfate fractionation and polyacrylamide gel electrophoresis, one activity not requiring flavine co-factors was precipitated by 50 to 70% ammonium sulfate concentration and identified with a slower-moving band on acrylamide gel electrophoresis; a second NADH2 oxidase activity was flavine mononucleotide (FMN) dependent and associated with a more rapidly moving band; it could only be partially precipitated by ammonium sulfate concentrations ranging from 50 to 100%. Studies with alternate electron acceptors indicated the presence of a menadione, a 2,6-dichlorophenol indophenol and a very weak ferricyanide oxido-reductase activity, but no cytochrome c oxido-reductase, in the cell-free preparations. The NADH2 oxidase activities of all fractions were relatively cyanide insensitive and were only minimally inhibited by flavoprotein and other respiratory chain inhibitors. H202 formation was negligible unless FMN, but not flavine adenine dinucleotide (FAD), was added to the crude NADH2 oxidase system; upon fractionation and electrophoresis, the H202 formation was associated with the FMN-dependent, more rapidly moving NADH2 oxidase band. This FMN-dependent NADH2 oxidase-H202 generating system may be a mechanism for the H202 formation observed during glucose oxidation in the intact organism. H202 has long been implicated in the pathogenicity of Mycoplasma pneumoniae for the human respiratory tract and cold agglutinin formation during infection. This peroxide has been identified by Somerson et al. (27) as the low-molecular-weight hemolysin that produces a diagnostic, rapid lysis of sheep or guinea pig red cells. However, the exact biochemical reaction or reactions in which H202 is formed have not been identified for either M. pneumoniae or any other H202-secreting mycoplasma. It seemed logical to examine cell-free extracts (CFE) of M. pneumoniae for reduced nicotinamide adenine dinucleotide (NADH2) and NADH phosphate (NADPH2) oxidase activity and especially the very active soluble NADH2 oxidase as a possible source of H202 formation. MATERIALS AND METHODSOrganisms, media, and cultivation. Exponentialphase organisms of the FH and Mac strains of M. pneumoniae were grown in Hayflick medium, washed once, and concentrated as 10% cell suspensions as previously described (19, 20). CFE. The concentrated cell suspensions of 4 x 109 colony-forming units per ml in 0.0067 M sodiumpotassium phosphate buffer (pH 7.0) were disrupted in early experiments by 10 min of sonic oscillation with a 10-kc Raytheon oscillator at 100 to 150 mA; more recent CFE preparations were obtained with a Branson W 185 sonic oscillator at a 20-kHz frequency and power output at step 5 for five 1-min periods, alternating with

show abstract

“…Our results clearly indicate that group 2 M. pneumoniae strains are more frequently isolated from clinical specimens than group 1 strains, since only 4 of 29 mycoplasma isolates examined belonged to group 1. Group 2 M. pneumoniae isolates include Mac and FH strains, which were originally isolated in California in the 1940s and 1950s (6,7,16), all TW isolates from the South Carolina study of military recruits in the early 1970s (24), the French isolates derived from clinical specimens in 1988, and the single isolate from an infectious site other than the respiratory tract (strain UTMB from synovial fluid; 4). It is interesting that M. pneumoniae isolates from a given study belong to the same group, as seen with all TW (group 2), PN (group 1), and French (group 2) strains ( Table 1).…”

mentioning

confidence: 99%

Molecular distinctions among clinical isolates of Mycoplasma pneumoniae

Dallo

Baseman

1990

J Clin Microbiol

View full text Add to dashboard Cite

Restriction enzyme fingerprinting of genomic DNA and Southern blots probed with subclones of the Mycoplasma pneumoniae cytadhesin Pl gene were used to characterize clinical isolates of M. pneumoniae. On the basis of the examination of 29 individual M. pneumoniae isolates, two distinct groups were established. Group 1, which displayed a 12-kilobase band following DNA digestion with HindIII, consisted of strain

show abstract

Replication of Mycoplasma pneumoniae in Broth Culture

Cited by 34 publications

References 9 publications

Cell biology of the mycoplasmas.

Cell biology of the mycoplasmas.

Reduced Nicotinamide Adenine Dinucleotide Oxidase Activity and H ₂ O ₂ Formation of Mycoplasma pneumoniae

Molecular distinctions among clinical isolates of Mycoplasma pneumoniae

Contact Info

Product

Resources

About

Replication of Mycoplasma pneumoniae in Broth Culture

Cited by 34 publications

References 9 publications

Cell biology of the mycoplasmas.

Cell biology of the mycoplasmas.

Reduced Nicotinamide Adenine Dinucleotide Oxidase Activity and H 2 O 2 Formation of Mycoplasma pneumoniae

Molecular distinctions among clinical isolates of Mycoplasma pneumoniae

Contact Info

Product

Resources

About

Reduced Nicotinamide Adenine Dinucleotide Oxidase Activity and H ₂ O ₂ Formation of Mycoplasma pneumoniae