Replication of influenza A virus in swine umbilical cord epithelial stem-like cells

Khatri, Mahesh; Chattha, Kuldeep S.

doi:10.4161/21505594.2014.983020

Cited by 9 publications

(6 citation statements)

References 55 publications

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“…Although transplacental passage of this virus is debated, embryonic cells can be affected through feto-maternal viral interference in signaling pathways controlling embryogenesis. Influenza replicates in certain multipotent stem cells 11–13 . Nevertheless, Influenza A virus (IAV) has limited replication in mouse embryonic stem cells (mESCs), but reduces their viability even at low multiplicity of infection (MOI), indicating the vulnerability of the blastocyst’s inner cell mass (ICM) to IAV 14 .…”

Section: Introductionmentioning

confidence: 99%

Influenza a virus-triggered autophagy decreases the pluripotency of human-induced pluripotent stem cells

et al. 2019

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Maternal influenza infection during pregnancy was reported multiple times as the possible cause of many defects and congenital anomalies. Apart from several cases of influenza-related miscarriage during various trimesters of pregnancy, some epidemiological data suggest a link between maternal influenza infection and genetic abnormalities in offspring. However, there are no reports yet describing how maternal influenza alters cellular pathways at early stages of development to result in congenital defects in the fetus. In the present study, using proteomic approaches, we utilized human-induced pluripotent stem cells (hiPSCs) for modeling intrablastocyst infection with influenza virus to not only investigate the vulnerability and responses of pluripotent stem cells to this virus but also to determine the possible impacts of influenza on pluripotency and signaling pathways controlling differentiation and embryogenesis. Our data indicated viral protein production in influenza A virus (IAV)-infected hiPSCs. However, viral replication was restricted in these cells, but cell viability and pluripotency were negatively affected. These events occurred simultaneously with an excessive level of IAV-induced autophagy as well as cytopathic effects. Quantitative SOMAscan screening also indicated that changes in the proteome of hiPSCs corresponded to abnormal differentiation in these cells. Taken together, our results showed that IAV-modulated reduction in hiPSC pluripotency is associated with significant activation of autophagy. Further investigations are required to explore the role of IAV-induced autophagy in leading pluripotent stem cells toward abnormal differentiation and impaired development in early stages of embryogenesis.

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Section: Introductionmentioning

confidence: 99%

Influenza a virus-triggered autophagy decreases the pluripotency of human-induced pluripotent stem cells

et al. 2019

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“…The most recent results published by Khatri and Chattha [25] indicate that both 2009 pandemic influenza virus and SIV replicate in swine umbilical cord epithelial stem cells (SUCECs). The authors have concluded that influenza virus can infect SUCECs and may transmit influenza virus from mother to fetus through the umbilical cord [25].…”

Section: In-inoculationmentioning

confidence: 99%

Pregnancy outcome and clinical status of gilts following experimental infection by H1N2, H3N2 and H1N1pdm09 influenza A viruses during the last month of gestation

Kwit

Pomorska‐Mól²,

Markowska-Daniel³

2015

Arch Virol

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The present study was planned to study the effect of various subtypes of swine influenza virus (SIV) circulating among pigs (H1N2, H3N2 and emerging pandemic strain of H1N1 influenza A virus (H1N1pdm09) on the course of pregnancy in naïve gilts experimentally infected during the last month of pregnancy. In addition, the clinical course of infection, distribution of viruses in various tissues (blood, placenta, fetal lung), and selected immunological, reproductive and productive parameters were also investigated. All SIV-inoculated gilts became infected. No abortions, stillbirths, intrauterine deaths or mummified fetuses were observed. No clinical signs of influenza virus infection or other disorders were observed in piglets born from infected and control gilts. There was a significant decrease in the number and frequency of lymphocytes in gilts inoculated with all influenza viruses. In general, the concentrations of IL-6, IL-10 and TNF-a were significantly higher in SIV-inoculated gilts as than in control animals, while IL-4 and IFN-c were not detected in plasma at any time post-inoculation in SIV-or mockinoculated gilts. No evidence for transplacental transmission of SIV was found. Viremia was also not observed in any of the infected females. On the basis of recent results, we hypothesize that pregnancy failure observed during SIV infection under field conditions is probably related to high fever and pro-inflammatory cytokines rather than a direct effect of the virus on the placenta, embryo or fetus.

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“…In the recent study, viral antigen was detected in blood vessels of the brain, spleen, kidneys, as well as in umbilical cord, due to transplacental passage. Some other swine viruses also replicate in umbilical cord due to their ability to cross the placenta of the pregnant sows, as previously described in infl uenza infection (Khatri and Chattha, 2015) and PRRS virus (Lager and Halbur, 1996).…”

Section: Discussionmentioning

confidence: 75%

Classical Swine Fever Virus Detection in Fetal Swine Tissues by Immunohistochemistry

Polaček

Đurđević

Radulović

et al. 2020

AVM

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The classical swine fever virus has the ability to cross the placental barrier, resulting in the infection of fetuses, which may consequently lead to persistent infection in piglets. The aim of this study was to report the lesions in fetuses naturally infected with CSFV during late gestation and clarify the nature of infected cells and the distribution of viral antigen in different tissues. A total of twenty-nine fetuses aged 82, 83 and 95 gestational days originating from three naturally CSFV infected sows were examined in this study. In all tested sows and their fetuses CSFV was detected using RT-PCR method. Immunohistochemistry method was used to detect viral antigen and monoclonal antibody WH303 was used on formalin fixed tissue samples of brain, spleen, heart, tonsils, kidney, ileocecal valve and umbilical cord. The most common lesions in the majority of fetuses were hyperemia, petechial haemorrhages in the skin, lymph nodes and kidneys. With the exception of myocardium, CSF viral antigen was detected in all the examined tissues. WH303 positive cells included endothelial cells, monocytes, macrophages and lymphocytes. The largest number of positive cells was found in kidneys in all of the examined fetuses. Reticular cells, macrophages, lymphocytes and endothelial cells in the spleen were also intensely and widely stained in most of the fetuses. These results showed that CSFV antigen can be detected in formalin-fixed, paraffin-embedded fetal tissue specimens originating from naturally CSFV infected sows by using monoclonal antibody WH303. Fetal kidneys proved to be a very useful organ for diagnosis of the CSF virus. Having that in mind, it is assumed that persistently infected piglets may shed a high amount of viral particles through urine. However, further research is needed to confirm this hypothesis.

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Replication of influenza A virus in swine umbilical cord epithelial stem-like cells

Cited by 9 publications

References 55 publications

Influenza a virus-triggered autophagy decreases the pluripotency of human-induced pluripotent stem cells

Influenza a virus-triggered autophagy decreases the pluripotency of human-induced pluripotent stem cells

Pregnancy outcome and clinical status of gilts following experimental infection by H1N2, H3N2 and H1N1pdm09 influenza A viruses during the last month of gestation

Classical Swine Fever Virus Detection in Fetal Swine Tissues by Immunohistochemistry

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