Replication of minichromosomes in a host in which chromosome replication is random

Eliasson, Åsa; Nordström, Kurt

doi:10.1046/j.1365-2958.1997.2981663.x

Cited by 18 publications

(15 citation statements)

References 24 publications

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“…It should also be mentioned that oriC minichromosomes retain a cell cycle‐specific replication pattern in a host in which chromosome replication is random in time, as shown by Eliasson et al. (1997) with a so‐called intP1 strain.…”

Section: Discussionmentioning

confidence: 99%

“…It should also be mentioned that oriC minichromosomes retain a cell cycle-specific replication pattern in a host in which chromosome replication is random in time, as shown by Eliasson et al (1997) with a so-called intP1 strain. This raises critical questions about the ability of the initiator titration model to explain the control of chromosome replication fully.…”

Section: Synchrony Sequestration and Eclipsementioning

confidence: 99%

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Eclipse period without sequestration in Escherichia coli

Olsson¹,

Dasgupta²,

Berg³

et al. 2002

Molecular Microbiology

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Summary The classical Meselson–Stahl density shift experiment was used to determine the length of the eclipse period in Escherichia coli, the minimum time period during which no new initiation is allowed from a newly replicated origin of chromosome replication, oriC. Populations of bacteria growing exponentially in heavy (15NH4+ and 13C6‐glucose) medium were shifted to light (14NH4+ and 12C6‐glucose) medium. The HH‐, HL‐ and LL‐DNA were separated by CsCl density gradient centrifugation, and their relative amounts were determined using radioactive gene‐specific probes. The eclipse period, estimated from the kinetics of conversion of HH‐DNA to HL‐ and LL‐DNA, turned out to be 0.60 generation times for the wild‐type strain. This was invariable for widely varying doubling times (35, 68 and 112 min) and was independent of the chromosome locus at which the eclipse period was measured. For strains with seqA, dam and damseqA mutants, the length of the eclipse period was 0.16, 0.40 and 0.32 generation times respectively. Thus, initiations from oriC were repressed for a considerable proportion of the generation time even when the sequestration function seemed to be severely compromised. The causal relationship between the length of the eclipse period and the synchrony of initiations from oriC is discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Synchrony Sequestration and Eclipsementioning

confidence: 99%

Eclipse period without sequestration in Escherichia coli

Olsson¹,

Dasgupta²,

Berg³

et al. 2002

Molecular Microbiology

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“…Consistent with this, major effects of transertion on the microviscosity of the membrane have been shown via inhibition of transcription and translation (Binenbaum et al, 1999). The second problem, the nature of the mechanism that initiates chromosome replication, is another long-standing mystery (Eliasson and Nordström, 1997; Wang et al, 2011). This mechanism in E. coli is generally attributed to the relative proportions of the DnaA ‘initiator’ protein in the ATP-DnaA form, which is active in initiation, and the ADP-DnaA, which is inactive (Castuma et al, 1993).…”

Section: Hyperstructuresmentioning

confidence: 99%

The membrane: transertion as an organizing principle in membrane heterogeneity

et al. 2015

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The bacterial membrane exhibits a significantly heterogeneous distribution of lipids and proteins. This heterogeneity results mainly from lipid–lipid, protein–protein, and lipid–protein associations which are orchestrated by the coupled transcription, translation and insertion of nascent proteins into and through membrane (transertion). Transertion is central not only to the individual assembly and disassembly of large physically linked groups of macromolecules (alias hyperstructures) but also to the interactions between these hyperstructures. We review here these interactions in the context of the processes in Bacillus subtilis and Escherichia coli of nutrient sensing, membrane synthesis, cytoskeletal dynamics, DNA replication, chromosome segregation, and cell division.

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“…Synchronous cell-cycle replication of up to 30 additional oriC copies is permitted, demonstrating that the levels of diffusible factors are not generally limiting (151). Cell cycle-specific minichromosome replication is not dependent on chromosomal oriC function, and minichromosomes are retained in cells with randomly replicating chromosomes, in which chromosomal oriC is replaced by a plasmid replication origin (62). Combined, these studies demonstrated that the wt oriC sequence contains all of the information needed to direct cell cycle-specific timing of initiation.…”

Section: Factors Involved In Initiation Of Chromosome Replicationmentioning

confidence: 99%

Initiation of DNA Replication

Leonard

Grimwade

2010

EcoSal Plus

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In recent years it has become clear that complex regulatory circuits control the initiation step of DNA replication by directing the assembly of a multicomponent molecular machine (the orisome) that separates DNA strands and loads replicative helicase at oriC , the unique chromosomal origin of replication. This chapter discusses recent efforts to understand the regulated protein-DNA interactions that are responsible for properly timed initiation of chromosome replication. It reviews information about newly identified nucleotide sequence features within Escherichia coli oriC and the new structural and biochemical attributes of the bacterial initiator protein DnaA. It also discusses the coordinated mechanisms that prevent improperly timed DNA replication. Identification of the genes that encoded the initiators came from studies on temperature-sensitive, conditional-lethal mutants of E. coli , in which two DNA replication-defective phenotypes, "immediate stop" mutants and "delayed stop" mutants, were identified. The kinetics of the delayed stop mutants suggested that the defective gene products were required specifically for the initiation step of DNA synthesis, and subsequently, two genes, dnaA and dnaC , were identified. The DnaA protein is the bacterial initiator, and in E. coli , the DnaC protein is required to load replicative helicase. Regulation of DnaA accessibility to oriC , the ordered assembly and disassembly of a multi-DnaA complex at oriC , and the means by which DnaA unwinds oriC remain important questions to be answered and the chapter discusses the current state of knowledge on these topics.

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Replication of minichromosomes in a host in which chromosome replication is random

Cited by 18 publications

References 24 publications

Eclipse period without sequestration in Escherichia coli

Eclipse period without sequestration in Escherichia coli

The membrane: transertion as an organizing principle in membrane heterogeneity

Initiation of DNA Replication

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